Detail


Protein	DNA-dependent protein kinase catalytic subunit
Gene	PRKDC
Status	Reviewed
Source	Colorectal tumors; Lung tumor; OVCAR-3 cell line (Ovarian cancer); Prostate
Years	2009-2015

Site	Identified Peptides	Year(Publication ID)
839	nLSSNEAISLEEIR	2014(14);
1350	nTSPEGWK	2015(1);
1859	SRFTKLnESTFDTQITK	2012(49);
2493	nDSQEIFK	2015(1);
2831	TLSEKNnITQKLLQDFNR	2009(68);

Sequence

1	11	21	31	41	51	61	71	81	91
MAGSGAGVRC	SLLRLQETLS	AADRCGAALA	GHQLIRGLGQ	ECVLSSSPAV	LALQTSLVFS	RDFGLLVFVR	KSLNSIEFRE	CREEILKFLC	IFLEKMGQKI
101	111	121	131	141	151	161	171	181	191
APYSVEIKNT	CTSVYTKDRA	AKCKIPALDL	LIKLLQTFRS	SRLMDEFKIG	ELFSKFYGEL	ALKKKIPDTV	LEKVYELLGL	LGEVHPSEMI	NNAENLFRAF
201	211	221	231	241	251	261	271	281	291
LGELKTQMTS	AVREPKLPVL	AGCLKGLSSL	LCNFTKSMEE	DPQTSREIFN	FVLKAIRPQI	DLKRYAVPSA	GLRLFALHAS	QFSTCLLDNY	VSLFEVLLKW
301	311	321	331	341	351	361	371	381	391
CAHTNVELKK	AALSALESFL	KQVSNMVAKN	AEMHKNKLQY	FMEQFYGIIR	NVDSNNKELS	IAIRGYGLFA	GPCKVINAKD	VDFMYVELIQ	RCKQMFLTQT
401	411	421	431	441	451	461	471	481	491
DTGDDRVYQM	PSFLQSVASV	LLYLDTVPEV	YTPVLEHLVV	MQIDSFPQYS	PKMQLVCCRA	IVKVFLALAA	KGPVLRNCIS	TVVHQGLIRI	CSKPVVLPKG
501	511	521	531	541	551	561	571	581	591
PESESEDHRA	SGEVRTGKWK	VPTYKDYVDL	FRHLLSSDQM	MDSILADEAF	FSVNSSSESL	NHLLYDEFVK	SVLKIVEKLD	LTLEIQTVGE	QENGDEAPGV
601	611	621	631	641	651	661	671	681	691
WMIPTSDPAA	NLHPAKPKDF	SAFINLVEFC	REILPEKQAE	FFEPWVYSFS	YELILQSTRL	PLISGFYKLL	SITVRNAKKI	KYFEGVSPKS	LKHSPEDPEK
701	711	721	731	741	751	761	771	781	791
YSCFALFVKF	GKEVAVKMKQ	YKDELLASCL	TFLLSLPHNI	IELDVRAYVP	ALQMAFKLGL	SYTPLAEVGL	NALEEWSIYI	DRHVMQPYYK	DILPCLDGYL
801	811	821	831	841	851	861	871	881	891
KTSALSDETK	NNWEVSALSR	AAQKGFNKVV	LKHLKKTKNL	SSNEAISLEE	IRIRVVQMLG	SLGGQINKNL	LTVTSSDEMM	KSYVAWDREK	RLSFAVPFRE
901	911	921	931	941	951	961	971	981	991
MKPVIFLDVF	LPRVTELALT	ASDRQTKVAA	CELLHSMVMF	MLGKATQMPE	GGQGAPPMYQ	LYKRTFPVLL	RLACDVDQVT	RQLYEPLVMQ	LIHWFTNNKK
1001	1011	1021	1031	1041	1051	1061	1071	1081	1091
FESQDTVALL	EAILDGIVDP	VDSTLRDFCG	RCIREFLKWS	IKQITPQQQE	KSPVNTKSLF	KRLYSLALHP	NAFKRLGASL	AFNNIYREFR	EEESLVEQFV
1101	1111	1121	1131	1141	1151	1161	1171	1181	1191
FEALVIYMES	LALAHADEKS	LGTIQQCCDA	IDHLCRIIEK	KHVSLNKAKK	RRLPRGFPPS	ASLCLLDLVK	WLLAHCGRPQ	TECRHKSIEL	FYKFVPLLPG
1201	1211	1221	1231	1241	1251	1261	1271	1281	1291
NRSPNLWLKD	VLKEEGVSFL	INTFEGGGCG	QPSGILAQPT	LLYLRGPFSL	QATLCWLDLL	LAALECYNTF	IGERTVGALQ	VLGTEAQSSL	LKAVAFFLES
1301	1311	1321	1331	1341	1351	1361	1371	1381	1391
IAMHDIIAAE	KCFGTGAAGN	RTSPQEGERY	NYSKCTVVVR	IMEFTTTLLN	TSPEGWKLLK	KDLCNTHLMR	VLVQTLCEPA	SIGFNIGDVQ	VMAHLPDVCV
1401	1411	1421	1431	1441	1451	1461	1471	1481	1491
NLMKALKMSP	YKDILETHLR	EKITAQSIEE	LCAVNLYGPD	AQVDRSRLAA	VVSACKQLHR	AGLLHNILPS	QSTDLHHSVG	TELLSLVYKG	IAPGDERQCL
1501	1511	1521	1531	1541	1551	1561	1571	1581	1591
PSLDLSCKQL	ASGLLELAFA	FGGLCERLVS	LLLNPAVLST	ASLGSSQGSV	IHFSHGEYFY	SLFSETINTE	LLKNLDLAVL	ELMQSSVDNT	KMVSAVLNGM
1601	1611	1621	1631	1641	1651	1661	1671	1681	1691
LDQSFRERAN	QKHQGLKLAT	TILQHWKKCD	SWWAKDSPLE	TKMAVLALLA	KILQIDSSVS	FNTSHGSFPE	VFTTYISLLA	DTKLDLHLKG	QAVTLLPFFT
1701	1711	1721	1731	1741	1751	1761	1771	1781	1791
SLTGGSLEEL	RRVLEQLIVA	HFPMQSREFP	PGTPRFNNYV	DCMKKFLDAL	ELSQSPMLLE	LMTEVLCREQ	QHVMEELFQS	SFRRIARRGS	CVTQVGLLES
1801	1811	1821	1831	1841	1851	1861	1871	1881	1891
VYEMFRKDDP	RLSFTRQSFV	DRSLLTLLWH	CSLDALREFF	STIVVDAIDV	LKSRFTKLNE	STFDTQITKK	MGYYKILDVM	YSRLPKDDVH	AKESKINQVF
1901	1911	1921	1931	1941	1951	1961	1971	1981	1991
HGSCITEGNE	LTKTLIKLCY	DAFTENMAGE	NQLLERRRLY	HCAAYNCAIS	VICCVFNELK	FYQGFLFSEK	PEKNLLIFEN	LIDLKRRYNF	PVEVEVPMER
2001	2011	2021	2031	2041	2051	2061	2071	2081	2091
KKKYIEIRKE	AREAANGDSD	GPSYMSSLSY	LADSTLSEEM	SQFDFSTGVQ	SYSYSSQDPR	PATGRFRRRE	QRDPTVHDDV	LELEMDELNR	HECMAPLTAL
2101	2111	2121	2131	2141	2151	2161	2171	2181	2191
VKHMHRSLGP	PQGEEDSVPR	DLPSWMKFLH	GKLGNPIVPL	NIRLFLAKLV	INTEEVFRPY	AKHWLSPLLQ	LAASENNGGE	GIHYMVVEIV	ATILSWTGLA
2201	2211	2221	2231	2241	2251	2261	2271	2281	2291
TPTGVPKDEV	LANRLLNFLM	KHVFHPKRAV	FRHNLEIIKT	LVECWKDCLS	IPYRLIFEKF	SGKDPNSKDN	SVGIQLLGIV	MANDLPPYDP	QCGIQSSEYF
2301	2311	2321	2331	2341	2351	2361	2371	2381	2391
QALVNNMSFV	RYKEVYAAAA	EVLGLILRYV	MERKNILEES	LCELVAKQLK	QHQNTMEDKF	IVCLNKVTKS	FPPLADRFMN	AVFFLLPKFH	GVLKTLCLEV
2401	2411	2421	2431	2441	2451	2461	2471	2481	2491
VLCRVEGMTE	LYFQLKSKDF	VQVMRHRDDE	RQKVCLDIIY	KMMPKLKPVE	LRELLNPVVE	FVSHPSTTCR	EQMYNILMWI	HDNYRDPESE	TDNDSQEIFK
2501	2511	2521	2531	2541	2551	2561	2571	2581	2591
LAKDVLIQGL	IDENPGLQLI	IRNFWSHETR	LPSNTLDRLL	ALNSLYSPKI	EVHFLSLATN	FLLEMTSMSP	DYPNPMFEHP	LSECEFQEYT	IDSDWRFRST
2601	2611	2621	2631	2641	2651	2661	2671	2681	2691
VLTPMFVETQ	ASQGTLQTRT	QEGSLSARWP	VAGQIRATQQ	QHDFTLTQTA	DGRSSFDWLT	GSSTDPLVDH	TSPSSDSLLF	AHKRSERLQR	APLKSVGPDF
2701	2711	2721	2731	2741	2751	2761	2771	2781	2791
GKKRLGLPGD	EVDNKVKGAA	GRTDLLRLRR	RFMRDQEKLS	LMYARKGVAE	QKREKEIKSE	LKMKQDAQVV	LYRSYRHGDL	PDIQIKHSSL	ITPLQAVAQR
2801	2811	2821	2831	2841	2851	2861	2871	2881	2891
DPIIAKQLFS	SLFSGILKEM	DKFKTLSEKN	NITQKLLQDF	NRFLNTTFSF	FPPFVSCIQD	ISCQHAALLS	LDPAAVSAGC	LASLQQPVGI	RLLEEALLRL
2901	2911	2921	2931	2941	2951	2961	2971	2981	2991
LPAELPAKRV	RGKARLPPDV	LRWVELAKLY	RSIGEYDVLR	GIFTSEIGTK	QITQSALLAE	ARSDYSEAAK	QYDEALNKQD	WVDGEPTEAE	KDFWELASLD
3001	3011	3021	3031	3041	3051	3061	3071	3081	3091
CYNHLAEWKS	LEYCSTASID	SENPPDLNKI	WSEPFYQETY	LPYMIRSKLK	LLLQGEADQS	LLTFIDKAMH	GELQKAILEL	HYSQELSLLY	LLQDDVDRAK
3101	3111	3121	3131	3141	3151	3161	3171	3181	3191
YYIQNGIQSF	MQNYSSIDVL	LHQSRLTKLQ	SVQALTEIQE	FISFISKQGN	LSSQVPLKRL	LNTWTNRYPD	AKMDPMNIWD	DIITNRCFFL	SKIEEKLTPL
3201	3211	3221	3231	3241	3251	3261	3271	3281	3291
PEDNSMNVDQ	DGDPSDRMEV	QEQEEDISSL	IRSCKFSMKM	KMIDSARKQN	NFSLAMKLLK	ELHKESKTRD	DWLVSWVQSY	CRLSHCRSRS	QGCSEQVLTV
3301	3311	3321	3331	3341	3351	3361	3371	3381	3391
LKTVSLLDEN	NVSSYLSKNI	LAFRDQNILL	GTTYRIIANA	LSSEPACLAE	IEEDKARRIL	ELSGSSSEDS	EKVIAGLYQR	AFQHLSEAVQ	AAEEEAQPPS
3401	3411	3421	3431	3441	3451	3461	3471	3481	3491
WSCGPAAGVI	DAYMTLADFC	DQQLRKEEEN	ASVIDSAELQ	AYPALVVEKM	LKALKLNSNE	ARLKFPRLLQ	IIERYPEETL	SLMTKEISSV	PCWQFISWIS
3501	3511	3521	3531	3541	3551	3561	3571	3581	3591
HMVALLDKDQ	AVAVQHSVEE	ITDNYPQAIV	YPFIISSESY	SFKDTSTGHK	NKEFVARIKS	KLDQGGVIQD	FINALDQLSN	PELLFKDWSN	DVRAELAKTP
3601	3611	3621	3631	3641	3651	3661	3671	3681	3691
VNKKNIEKMY	ERMYAALGDP	KAPGLGAFRR	KFIQTFGKEF	DKHFGKGGSK	LLRMKLSDFN	DITNMLLLKM	NKDSKPPGNL	KECSPWMSDF	KVEFLRNELE
3701	3711	3721	3731	3741	3751	3761	3771	3781	3791
IPGQYDGRGK	PLPEYHVRIA	GFDERVTVMA	SLRRPKRIII	RGHDEREHPF	LVKGGEDLRQ	DQRVEQLFQV	MNGILAQDSA	CSQRALQLRT	YSVVPMTSRL
3801	3811	3821	3831	3841	3851	3861	3871	3881	3891
GLIEWLENTV	TLKDLLLNTM	SQEEKAAYLS	DPRAPPCEYK	DWLTKMSGKH	DVGAYMLMYK	GANRTETVTS	FRKRESKVPA	DLLKRAFVRM	STSPEAFLAL
3901	3911	3921	3931	3941	3951	3961	3971	3981	3991
RSHFASSHAL	ICISHWILGI	GDRHLNNFMV	AMETGGVIGI	DFGHAFGSAT	QFLPVPELMP	FRLTRQFINL	MLPMKETGLM	YSIMVHALRA	FRSDPGLLTN
4001	4011	4021	4031	4041	4051	4061	4071	4081	4091
TMDVFVKEPS	FDWKNFEQKM	LKKGGSWIQE	INVAEKNWYP	RQKICYAKRK	LAGANPAVIT	CDELLLGHEK	APAFRDYVAV	ARGSKDHNIR	AQEPESGLSE
4101	4111	4121
ETQVKCLMDQ	ATDPNILGRT	WEGWEPWM

UniProtKB-P78527

Glycosites

Sequence

Reference

ID	Publication
1	Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
14	Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
49	Kim JY, Kim S-K, Kang D, Moon MH: Dual Lectin-Based Size Sorting Strategy to Enrich Targeted N-Glycopeptides by Asymmetrical Flow Field-Flow Fractionation: Profiling Lung Cancer Biomarkers. Analytical Chemistry 2012, 84:5343-5350.
68	Zhang L, Xu Y, Yao H, Xie L, Yao J, Lu H, Yang P: Boronic Acid Functionalized Core-Satellite Composite Nanoparticles for Advanced Enrichment of Glycopeptides and Glycoproteins. Chemistry-a European Journal 2009, 15:10158-10166.