Contains      

UniProtKB-Q02985

Protein Complement factor H-related protein 3
Gene CFHR3
Status Reviewed
Source Breast tumor; Colorectal tumor; Liver; Liver tumor (HCC); Ovarian tumor; Plasma; Prostate; Prostate tumor; Serum; Serum (HCC); Urine; plasma
Years 2007-2015

Glycosites

Site Identified Peptides Year(Publication ID)
108 FVQGnSTE 2014(33);
108 KFVQGnSTE 2014(33);
108 VQGnSTEVACHPGY 2014(33);
108 FVQGnSTEVACHPGY 2014(33);
108 KFVQGnSTEVACHPGY 2014(33);
108 VQGnSTEVACHPGYGL 2014(33);
108 VQGnSTEVACHPGYGLPK 2014(33);
108 FVQGnSTEVACHPGYGLPK 2009(64); 2010(40); 2012(53); 2013(38); 2013(38); 2013(42); 2013(45); 2014(13); 2014(14); 2014(35); 2014(22); 2014(33); 2015(unpublished);2015(unpublished);2015(unpublished);2007(unpublished);2007;2007;2007;
108 KFVQGnSTEVACHPGYGIPK 2014(32);
108 KFVQGnSTEVACHPGYGLPK 2007(unpublished);2009(64); 2010(40); 2013(38); 2013(42); 2013(45); 2014(13); 2014(33); 2015(unpublished);2015(unpublished);
185 ATADGnSSGSITCL 2014(33);
185 ATADGnSSGSITCLQNGW 2014(33);
185 KCKPGYATADGnSSGSITCL 2014(33);
185 CKPGYATADGnSSGSITCLQNGWSAQPICINSSEK 2015(unpublished);
205 InSSEKCGPPPP 2009(64);
205 SAQPICInSSEK 2014(33);
205 QNGWSAQPICInSSEK 2014(33);
205 SAQPICInSSEKCGPPPPISNGDTTSF 2014(33);
205 CKPGYATADGNSSGSITCLQNGWSAQPICInSSEK 2015(unpublished);
309 GYNAnTSIL 2014(33);
309 NAnTSILSF 2014(33);
309 LGYNAnTSIL 2014(33);
309 LGYNAnTSILSF 2014(33);
309 MCKLGYNAnTSIL 2009(64);
309 NAnTSILSFQAVCR 2014(33);
309 LGYNAnTSILSFQAVCR 2007(13); 2014(33); 2014(4); 2015(8); 2015(unpublished);2015(unpublished);2015(unpublished);2007;2007;2007;

Sequence

1112131415161718191
MLLLINVILTLWVSCANGQVKPCDFPDIKHGGLFHENMRRPYFPVAVGKYYSYYCDEHFETPSGSYWDYIHCTQNGWSPAVPCLRKCYFPYLENGYNQNY
101111121131141151161171181191
GRKFVQGNSTEVACHPGYGLPKAQTTVTCTEKGWSPTPRCIRVRTCSKSDIEIENGFISESSSIYILNKEIQYKCKPGYATADGNSSGSITCLQNGWSAQ
201211221231241251261271281291
PICINSSEKCGPPPPISNGDTTSFLLKVYVPQSRVEYQCQPYYELQGSNYVTCSNGEWSEPPRCIHPCIITEENMNKNNIKLKGRSDRKYYAKTGDTIEF
301311321
MCKLGYNANTSILSFQAVCREGIVEYPRCE

Reference

ID Publication
4Cheow ESH, Sim KH, de Kleijn D, Lee CN, Sorokin V, Sze SK: Simultaneous Enrichment of Plasma Soluble and Extracellular Vesicular Glycoproteins Using Prolonged Ultracentrifugation-Electrostatic Repulsion-hydrophilic Interaction Chromatography (PUC-ERLIC) Approach. Molecular & Cellular Proteomics 2015, 14:1657-1671.
8Ma C, Zhang Q, Qu JY, Zhao XY, Li X, Liu YP, Wang PG: A precise approach in large scale core-fucosylated glycoprotein identification with low- and high-normalized collision energy. Journal of Proteomics 2015, 114:61-70.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
35Chen J, Shah P, Zhang H: Solid phase extraction of N-linked glycopeptides using hydrazide tip. Analytical chemistry 2013, 85:10670-10674.
38Kaji H, Ocho M, Togayachi A, Kuno A, Sogabe M, Ohkura T, Nozaki H, Angata T, Chiba Y, Ozaki H, et al: Glycoproteomic Discovery of Serological Biomarker Candidates for HCV/HBV Infection-Associated Liver Fibrosis and Hepatocellular Carcinoma. Journal of Proteome Research 2013, 12:2630-2640.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
42Ma C, Zhao X, Han H, Tong W, Zhang Q, Qin P, Chang C, Peng B, Ying W, Qian X: N-linked glycoproteome profiling of human serum using tandem enrichment and multiple fraction concatenation. Electrophoresis 2013, 34:2440-2450.
45Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991.
53Zhu J, Wang F, Chen R, Cheng K, Xu B, Guo Z, Liang X, Ye M, Zou H: Centrifugation Assisted Microreactor Enables Facile Integration of Trypsin Digestion, Hydrophilic Interaction Chromatography Enrichment, and On-Column Deglycosylation for Rapid and Sensitive N-Glycoproteome Analysis. Analytical Chemistry 2012, 84:5146-5153.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.