Detail

UniProtKB-Q04721


Protein	Neurogenic locus notch homolog protein 2
Gene	NOTCH2
Status	Reviewed
Source	Breast cancer cell lines; Breast cancer xenografts; Breast tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); Hela cell line (cervical cancer); Liver; OVCAR-3 cell line (Ovarian cancer); Prostate tumor; Serum; Urine; UrineJurkat T cell line
Years	2007-2015

Glycosites

Site	Identified Peptides	Year(Publication ID)
46	nGTGYCK	2015(1);
46	GMCVTYHnGTGY	2014(33);
46	GMCVTYHnGTGYCK	2014(33);
46	DGYEPCVNEGMCVTYHnGTGYCK	2007(45); 2013(13); 2014(16); 2014(33); 2014(1); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2007(unpublished);2007;2007;2007;
155	nGSTCTTVANQFSCK	2015(1);
733	nCTGGLSGYK	2015(1);
2066	LLDEYnVTPSP	2007(unpublished);

Sequence

1	11	21	31	41	51	61	71	81	91
MPALRPALLW	ALLALWLCCA	APAHALQCRD	GYEPCVNEGM	CVTYHNGTGY	CKCPEGFLGE	YCQHRDPCEK	NRCQNGGTCV	AQAMLGKATC	RCASGFTGED
101	111	121	131	141	151	161	171	181	191
CQYSTSHPCF	VSRPCLNGGT	CHMLSRDTYE	CTCQVGFTGK	ECQWTDACLS	HPCANGSTCT	TVANQFSCKC	LTGFTGQKCE	TDVNECDIPG	HCQHGGTCLN
201	211	221	231	241	251	261	271	281	291
LPGSYQCQCP	QGFTGQYCDS	LYVPCAPSPC	VNGGTCRQTG	DFTFECNCLP	GFEGSTCERN	IDDCPNHRCQ	NGGVCVDGVN	TYNCRCPPQW	TGQFCTEDVD
301	311	321	331	341	351	361	371	381	391
ECLLQPNACQ	NGGTCANRNG	GYGCVCVNGW	SGDDCSENID	DCAFASCTPG	STCIDRVASF	SCMCPEGKAG	LLCHLDDACI	SNPCHKGALC	DTNPLNGQYI
401	411	421	431	441	451	461	471	481	491
CTCPQGYKGA	DCTEDVDECA	MANSNPCEHA	GKCVNTDGAF	HCECLKGYAG	PRCEMDINEC	HSDPCQNDAT	CLDKIGGFTC	LCMPGFKGVH	CELEINECQS
501	511	521	531	541	551	561	571	581	591
NPCVNNGQCV	DKVNRFQCLC	PPGFTGPVCQ	IDIDDCSSTP	CLNGAKCIDH	PNGYECQCAT	GFTGVLCEEN	IDNCDPDPCH	HGQCQDGIDS	YTCICNPGYM
601	611	621	631	641	651	661	671	681	691
GAICSDQIDE	CYSSPCLNDG	RCIDLVNGYQ	CNCQPGTSGV	NCEINFDDCA	SNPCIHGICM	DGINRYSCVC	SPGFTGQRCN	IDIDECASNP	CRKGATCING
701	711	721	731	741	751	761	771	781	791
VNGFRCICPE	GPHHPSCYSQ	VNECLSNPCI	HGNCTGGLSG	YKCLCDAGWV	GINCEVDKNE	CLSNPCQNGG	TCDNLVNGYR	CTCKKGFKGY	NCQVNIDECA
801	811	821	831	841	851	861	871	881	891
SNPCLNQGTC	FDDISGYTCH	CVLPYTGKNC	QTVLAPCSPN	PCENAAVCKE	SPNFESYTCL	CAPGWQGQRC	TIDIDECISK	PCMNHGLCHN	TQGSYMCECP
901	911	921	931	941	951	961	971	981	991
PGFSGMDCEE	DIDDCLANPC	QNGGSCMDGV	NTFSCLCLPG	FTGDKCQTDM	NECLSEPCKN	GGTCSDYVNS	YTCKCQAGFD	GVHCENNINE	CTESSCFNGG
1001	1011	1021	1031	1041	1051	1061	1071	1081	1091
TCVDGINSFS	CLCPVGFTGS	FCLHEINECS	SHPCLNEGTC	VDGLGTYRCS	CPLGYTGKNC	QTLVNLCSRS	PCKNKGTCVQ	KKAESQCLCP	SGWAGAYCDV
1101	1111	1121	1131	1141	1151	1161	1171	1181	1191
PNVSCDIAAS	RRGVLVEHLC	QHSGVCINAG	NTHYCQCPLG	YTGSYCEEQL	DECASNPCQH	GATCSDFIGG	YRCECVPGYQ	GVNCEYEVDE	CQNQPCQNGG
1201	1211	1221	1231	1241	1251	1261	1271	1281	1291
TCIDLVNHFK	CSCPPGTRGL	LCEENIDDCA	RGPHCLNGGQ	CMDRIGGYSC	RCLPGFAGER	CEGDINECLS	NPCSSEGSLD	CIQLTNDYLC	VCRSAFTGRH
1301	1311	1321	1331	1341	1351	1361	1371	1381	1391
CETFVDVCPQ	MPCLNGGTCA	VASNMPDGFI	CRCPPGFSGA	RCQSSCGQVK	CRKGEQCVHT	ASGPRCFCPS	PRDCESGCAS	SPCQHGGSCH	PQRQPPYYSC
1401	1411	1421	1431	1441	1451	1461	1471	1481	1491
QCAPPFSGSR	CELYTAPPST	PPATCLSQYC	ADKARDGVCD	EACNSHACQW	DGGDCSLTME	NPWANCSSPL	PCWDYINNQC	DELCNTVECL	FDNFECQGNS
1501	1511	1521	1531	1541	1551	1561	1571	1581	1591
KTCKYDKYCA	DHFKDNHCDQ	GCNSEECGWD	GLDCAADQPE	NLAEGTLVIV	VLMPPEQLLQ	DARSFLRALG	TLLHTNLRIK	RDSQGELMVY	PYYGEKSAAM
1601	1611	1621	1631	1641	1651	1661	1671	1681	1691
KKQRMTRRSL	PGEQEQEVAG	SKVFLEIDNR	QCVQDSDHCF	KNTDAAAALL	ASHAIQGTLS	YPLVSVVSES	LTPERTQLLY	LLAVAVVIIL	FIILLGVIMA
1701	1711	1721	1731	1741	1751	1761	1771	1781	1791
KRKRKHGSLW	LPEGFTLRRD	ASNHKRREPV	GQDAVGLKNL	SVQVSEANLI	GTGTSEHWVD	DEGPQPKKVK	AEDEALLSEE	DDPIDRRPWT	QQHLEAADIR
1801	1811	1821	1831	1841	1851	1861	1871	1881	1891
RTPSLALTPP	QAEQEVDVLD	VNVRGPDGCT	PLMLASLRGG	SSDLSDEDED	AEDSSANIIT	DLVYQGASLQ	AQTDRTGEMA	LHLAARYSRA	DAAKRLLDAG
1901	1911	1921	1931	1941	1951	1961	1971	1981	1991
ADANAQDNMG	RCPLHAAVAA	DAQGVFQILI	RNRVTDLDAR	MNDGTTPLIL	AARLAVEGMV	AELINCQADV	NAVDDHGKSA	LHWAAAVNNV	EATLLLLKNG
2001	2011	2021	2031	2041	2051	2061	2071	2081	2091
ANRDMQDNKE	ETPLFLAARE	GSYEAAKILL	DHFANRDITD	HMDRLPRDVA	RDRMHHDIVR	LLDEYNVTPS	PPGTVLTSAL	SPVICGPNRS	FLSLKHTPMG
2101	2111	2121	2131	2141	2151	2161	2171	2181	2191
KKSRRPSAKS	TMPTSLPNLA	KEAKDAKGSR	RKKSLSEKVQ	LSESSVTLSP	VDSLESPHTY	VSDTTSSPMI	TSPGILQASP	NPMLATAAPP	APVHAQHALS
2201	2211	2221	2231	2241	2251	2261	2271	2281	2291
FSNLHEMQPL	AHGASTVLPS	VSQLLSHHHI	VSPGSGSAGS	LSRLHPVPVP	ADWMNRMEVN	ETQYNEMFGM	VLAPAEGTHP	GIAPQSRPPE	GKHITTPREP
2301	2311	2321	2331	2341	2351	2361	2371	2381	2391
LPPIVTFQLI	PKGSIAQPAG	APQPQSTCPP	AVAGPLPTMY	QIPEMARLPS	VAFPTAMMPQ	QDGQVAQTIL	PAYHPFPASV	GKYPTPPSQH	SYASSNAAER
2401	2411	2421	2431	2441	2451	2461	2471
TPSHSGHLQG	EHPYLTPSPE	SPDQWSSSSP	HSASDWSDVT	TSPTPGGAGG	GQRGPGTHMS	EPPHNNMQVY	A

Reference

ID	Publication
1	Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
12	Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
13	Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
16	Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
33	Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
45	Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991.