Contains      

UniProtKB-Q12913

Protein Receptor-type tyrosine-protein phosphatase eta
Gene PTPRJ
Status Reviewed
Source 22Rv1 cell xenograft (prostate cancer); Breast Cancer cell lines; Breast cancer xenografts; Breast cancer xenograftsPBMC Macrophage cells; Breast tumor; Colorectal tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); HCC cell lines (Liver); HCCLM3 cell line (Liver); HEK293 cell line (embryonic kidney); HEK293T cell line (embryonic kidney); Hela cell line (cervical cancer); HuH-7 cell line (Liver); Human; LNCap/PC3 cell lines (Prostate cancer); Liver; Liver tumor (HCC); OVCAR-3 cell line (Ovarian cancer); Ovarian tumor; Pancreatic islets; Plasma; PlasmaJurkat T cell line; Platelet; Platelet Plasma membranes; Prostate; Prostate tumor; SKOV-3 cell line (Ovarian cancer); SKOV-3 cell line (Ovarian cancer)Jurkat T cell line; SW1990 cell line (Pancreatic cancer); Serum; Serum (HCC); Spermatozoa; Urine; plasma
Years 2005-2015

Glycosites

Site Identified Peptides Year(Publication ID)
104 nGTDGASQK 2015(1);
104 TPEQGSnGTDGASQK 2005( 81); 2007(74); 2013(34); 2013(34); 2013(40); 2014(13); 2014(14); 2014(17); 2014(22); 2014(33); 2015(2); 2015(3); 2015(unpublished);2015(unpublished);2007;2007;
104 QGSnGTDGASQKTPSSTGPSPVFD 2013(42);
104 TPEQGSnGTDGASQKTPSSTGPSPVFDIK 2014(16);
142 KSnDTAASEY 2014(33);
142 SnDTAASEYK 2005( 81); 2007(74); 2013(40); 2013(45); 2014(13); 2014(16); 2014(22); 2014(33); 2015(2); 2015(3); 2015(10); 2015(unpublished);2015(unpublished);2015;2015;2015;2007;2007;
142 TWKSnDTAASEY 2014(33);
142 IITWKSnDTAASE 2013(37);
142 SnDTAASEYKYVVK 2015(unpublished);
142 AVSISPTNVILTWKSnDTAASEYK 2015(unpublished);
142 AVSISPTNVILTWKSnDTAASEYKYVVK 2015(unpublished);
172 CnITGLRPATSY 2014(33);
172 TITVVHQPWCnITGLR 2015(unpublished);
172 TITVVHQPWCnITGLRPATSYVFSITPGIGNETWGDPR 2015(1);
192 nETWGDPR 2015(1);
192 SITPGIGnETWGDPR 2014(33);
192 VFSITPGIGnETWGDPR 2014(33);
192 TITVVHQPWCNITGLRPATSYVFSITPGIGnETWGDPR 2015(1);
231 nGTASCR 2015(1);
231 ISWSNGnGTASCR 2013(37);
231 SNGnGTASCRVLL 2014(33);
231 AAISWSNGnGTASCR 2014(16);
231 AALSWSNGnGTASCR 2009(63); 2012(48); 2013(40); 2015(1); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);
231 KAAISWSNGnGTASCR 2014(16);
231 KAALSWSNGnGTASCR 2015(unpublished);2015(unpublished);2015(12);
258 LQVnISGLKPGVQY 2007(33); 2014(unpublished);2007(unpublished);2007;
258 LQVnISGLKPGVQYNINPY 2015(unpublished);
258 IQVnISGIKPGVQYNINPYIIQSNK 2014(16);
258 LQVnISGLKPGVQYNINPYLLQSNK 2015(unpublished);
258 LQVnISGLKPGVQYNINPYLLQSNKTK 2015(unpublished);
278 IQVNISGIKPGVQYNINPYIIQSnK 2014(16);
278 LQVNISGLKPGVQYNINPYLLQSnK 2015(unpublished);
278 LQVNISGLKPGVQYNINPYLLQSnKTK 2015(unpublished);
342 LVGLEPGTRYnATVYSQAAN 2015(6);
342 YnATVYSQAANGTEGQPQAIEFR 2005( 81); 2007(74); 2013(34); 2013(34); 2013(34); 2013(40); 2014(13); 2014(16); 2015(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015;2015;2007;2007;2007;2007;
342 DTEVLLVGLEPGTRYnATVYSQAANGTEGQPQAIEFR 2015(unpublished);
351 nGTEGQPQAIEFR 2015(1);
351 SQAAnGTEGQPQAIEF 2014(33);
351 YNATVYSQAAnGTEGQPQAIEFR 2005( 81); 2007(74); 2013(34); 2013(34); 2013(34); 2013(40); 2014(13); 2014(16); 2015(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015;2015;2007;2007;2007;2007;
351 DTEVLLVGLEPGTRYNATVYSQAAnGTEGQPQAIEFR 2015(unpublished);
376 DVTAVnISATSL 2014(33);
376 nISATSLTLIWK 2015(1);
391 nESSSNYTYK 2015(1);
391 VSDnESSSNYTYK 2005( 81); 2007(74); 2012(48); 2012(50); 2013(34); 2013(34); 2013(34); 2013(38); 2013(40); 2013(45); 2014(13); 2014(13); 2014(14); 2014(16); 2014(22); 2015(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015;2015;2015;2015;2007;2007;2007;
391 WKVSDnESSSNYTYKIHVAG 2015(6);
396 NESSSnYTYK 2015(1);
396 VSDNESSSnYTYK 2005( 81); 2007(74); 2012(48); 2012(50); 2013(34); 2013(34); 2013(34); 2013(38); 2013(40); 2013(45); 2014(13); 2014(13); 2014(14); 2014(16); 2014(22); 2015(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015;2015;2015;2015;2007;2007;2007;
396 WKVSDNESSSnYTYKIHVAG 2015(6);
413 TDSSNLnVSEPR 2014(33);
413 TDSSNInVSEPRA 2013(37);
413 GETDSSNLnVSEPR 2014(33);
413 IHVAGETDSSNInVSEPR 2013(43); 2014(16); 2014(32); 2014(32);
413 IHVAGETDSSNLnVSEPR 2005( 81); 2007(74); 2007(63); 2009(64); 2009(40); 2010(57); 2011(47); 2012(48); 2012(34); 2013(34); 2013(34); 2013(38); 2013(38); 2013(40); 2013(45); 2013(13); 2014(13); 2014(14); 2014(17); 2014(22); 2014(33); 2014(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015;2015;2015;2015;2015;2015;2007;2007;2007;2007;
413 VAGETDSSNLnVSEPRAVIP 2015(6);
431 SSTFYnITVCPVIGDIEGTPGFIQVHTPPVPVSDFR 2014(16);
431 SSTFYnITVCPVLGDIEGTPGFLQVHTPPVPVSDFR 2014(13); 2015(unpublished);2015(1); 2015(unpublished);
501 nQSIIIGGLFPGTK 2015(1);
501 ITTnQSIIIGGLFPGTK 2014(33);
501 VEITTnQSIIIGGLFPGTK 2014(33); 2015(unpublished);2015(1); 2015(unpublished);2015(2); 2015(unpublished);2015(unpublished);2015(unpublished);
525 GPnGTEGASR 2007(74); 2014(16); 2014(33); 2015(unpublished);
582 HGSnHTSTYDK 2013(40); 2014(16); 2015(unpublished);
582 ESKHGSnHTSTY 2014(33);
582 HLVIESKHGSnHTSTY 2014(33);
603 nITISPEVDHVW 2014(33);
618 nSTAQYTRPS 2015(1);
618 VDHVWGDPnSTAQY 2014(33);
628 TRPSnVSNIDVSTNTTAATL 2014(33);
637 TRPSNVSNIDVSTnTTAATL 2014(33);
761 nATHLESCSSE 2015(1);
761 VSSGAWNnATHLE 2014(33);
790 nISITTVSCGK 2015(1);
824 nITSVSHNSVK 2015(1);
824 NTCTTGITDPPPPDGSPnITSVSHNSVK 2007(48); 2012(34); 2013(34); 2013(13); 2014(15); 2014(16); 2014(17); 2014(33); 2014(1); 2015(12); 2015(unpublished);2015(unpublished);2015;2015;2007;2007;
910 EIDVGnESTTL 2014(33);
910 KYEIDVGnESTTL 2014(33);
910 YEIDVGnESTTLGYYNGK 2014(33); 2015(1); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);
910 YEIDVGnESTTLGYYNGKLEPLGSYR 2015(1); 2015(unpublished);
910 SQSLSEVLKYEIDVGnESTTLGYYNGK 2015(unpublished);
910 SQSLSEVLKYEIDVGnESTTLGYYNGKLEPLGSYR 2015(unpublished);
937 nITFHPQNK 2015(1);
937 ACVAGFTnITFHPQNK 2009(64); 2013(34); 2013(45); 2014(16); 2014(33); 2015(unpublished);2015(1); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);

Sequence

1112131415161718191
MKPAAREARLPPRSPGLRWALPLLLLLLRLGQILCAGGTPSPIPDPSVATVATGENGITQISSTAESFHKQNGTGTPQVETNTSEDGESSGANDSLRTPE
101111121131141151161171181191
QGSNGTDGASQKTPSSTGPSPVFDIKAVSISPTNVILTWKSNDTAASEYKYVVKHKMENEKTITVVHQPWCNITGLRPATSYVFSITPGIGNETWGDPRV
201211221231241251261271281291
IKVITEPIPVSDLRVALTGVRKAALSWSNGNGTASCRVLLESIGSHEELTQDSRLQVNISGLKPGVQYNINPYLLQSNKTKGDPLGTEGGLDASNTERSR
301311321331341351361371381391
AGSPTAPVHDESLVGPVDPSSGQQSRDTEVLLVGLEPGTRYNATVYSQAANGTEGQPQAIEFRTNAIQVFDVTAVNISATSLTLIWKVSDNESSSNYTYK
401411421431441451461471481491
IHVAGETDSSNLNVSEPRAVIPGLRSSTFYNITVCPVLGDIEGTPGFLQVHTPPVPVSDFRVTVVSTTEIGLAWSSHDAESFQMHITQEGAGNSRVEITT
501511521531541551561571581591
NQSIIIGGLFPGTKYCFEIVPKGPNGTEGASRTVCNRTVPSAVFDIHVVYVTTTEMWLDWKSPDGASEYVYHLVIESKHGSNHTSTYDKAITLQGLIPGT
601611621631641651661671681691
LYNITISPEVDHVWGDPNSTAQYTRPSNVSNIDVSTNTTAATLSWQNFDDASPTYSYCLLIEKAGNSSNATQVVTDIGITDATVTELIPGSSYTVEIFAQ
701711721731741751761771781791
VGDGIKSLEPGRKSFCTDPASMASFDCEVVPKEPALVLKWTCPPGANAGFELEVSSGAWNNATHLESCSSENGTEYRTEVTYLNFSTSYNISITTVSCGK
801811821831841851861871881891
MAAPTRNTCTTGITDPPPPDGSPNITSVSHNSVKVKFSGFEASHGPIKAYAVILTTGEAGHPSADVLKYTYEDFKKGASDTYVTYLIRTEEKGRSQSLSE
901911921931941951961971981991
VLKYEIDVGNESTTLGYYNGKLEPLGSYRACVAGFTNITFHPQNKGLIDGAESYVSFSRYSDAVSLPQDPGVICGAVFGCIFGALVIVTVGGFIFWRKKR
1001101110211031104110511061107110811091
KDAKNNEVSFSQIKPKKSKLIRVENFEAYFKKQQADSNCGFAEEYEDLKLVGISQPKYAAELAENRGKNRYNNVLPYDISRVKLSVQTHSTDDYINANYM
1101111111211131114111511161117111811191
PGYHSKKDFIATQGPLPNTLKDFWRMVWEKNVYAIIMLTKCVEQGRTKCEEYWPSKQAQDYGDITVAMTSEIVLPEWTIRDFTVKNIQTSESHPLRQFHF
1201121112211231124112511261127112811291
TSWPDHGVPDTTDLLINFRYLVRDYMKQSPPESPILVHCSAGVGRTGTFIAIDRLIYQIENENTVDVYGIVYDLRMHRPLMVQTEDQYVFLNQCVLDIVR
1301131113211331
SQKDSKVDLIYQNTTAMTIYENLAPVTTFGKTNGYIA

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
3Tian Y, Almaraz RT, Choi CH, Li QK, Saeui C, Li D, Shah P, Bhattacharya R, Yarema KJ, Zhang H: Identification of sialylated glycoproteins from metabolically oligosaccharide engineered pancreatic cells. Clinical proteomics 2015, 12:11.
6Kim DS, Hahn Y: The acquisition of novel N-glycosylation sites in conserved proteins during human evolution. Bmc Bioinformatics 2015, 16.
10Tan ZJ, Yin HD, Nie S, Lin ZX, Zhu JH, Ruffin MT, Anderson MA, Simone DM, Lubman DM: Large-Scale Identification of Core-Fucosylated Glycopeptide Sites in Pancreatic Cancer Serum Using Mass Spectrometry. Journal of Proteome Research 2015, 14:1968-1978.
12Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
15Chen R, Seebun D, Ye M, Zou H, Figeys D: Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS. Journal of Proteomics 2014, 103:194-203.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
17Fang C, Xiong Z, Qin H, Huang G, Liu J, Ye M, Feng S, Zou H: One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides. Analytica Chimica Acta 2014, 841:99-105.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
38Kaji H, Ocho M, Togayachi A, Kuno A, Sogabe M, Ohkura T, Nozaki H, Angata T, Chiba Y, Ozaki H, et al: Glycoproteomic Discovery of Serological Biomarker Candidates for HCV/HBV Infection-Associated Liver Fibrosis and Hepatocellular Carcinoma. Journal of Proteome Research 2013, 12:2630-2640.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
42Ma C, Zhao X, Han H, Tong W, Zhang Q, Qin P, Chang C, Peng B, Ying W, Qian X: N-linked glycoproteome profiling of human serum using tandem enrichment and multiple fraction concatenation. Electrophoresis 2013, 34:2440-2450.
43Wang GG, Wu YB, Zhou T, Guo YS, Zheng B, Wang J, Bi Y, Liu FJ, Zhou ZM, Guo XJ, Sha JH: Mapping of the N-Linked Glycoproteome of Human Spermatozoa. Journal of Proteome Research 2013, 12:5750-5759.
45Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991.
47Almaraz RT, Tian Y, Bhattarcharya R, Tan E, Chen S-H, Dallas MR, Chen L, Zhang Z, Zhang H, Konstantopoulos K: Metabolic flux increases glycoprotein sialylation: implications for cell adhesion and cancer metastasis. Molecular & Cellular Proteomics 2012, 11:M112. 017558.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
50Whitmore TE, Peterson A, Holzman T, Eastham A, Amon L, McIntosh M, Ozinsky A, Nelson PS, Martin DB: Integrative Analysis of N-Linked Human Glycoproteomic Data Sets Reveals PTPRF Ectodomain as a Novel Plasma Biomarker Candidate for Prostate Cancer. Journal of Proteome Research 2012, 11:2653-2665.
57Chen Y, Cao J, Yan G, Lu H, Yang P: Two-step protease digestion and glycopeptide capture approach for accurate glycosite identification and glycoprotein sequence coverage improvement. Talanta 2011, 85:70-75.
63Cao J, Shen C, Wang H, Shen H, Chen Y, Nie A, Yan G, Lu H, Liu Y, Yang P: Identification of N-Glycosylation Sites on Secreted Proteins of Human Hepatocellular Carcinoma Cells with a Complementary Proteomics Approach. Journal of Proteome Research 2009, 8:662-672.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
74Lewandrowski U, Zahedi RP, Moebius J, Walter U, Sickmann A: Enhanced N-glycosylation site analysis of Sialoglycopeptides by strong cation exchange prefractionation applied to platelet plasma membranes. Molecular & Cellular Proteomics 2007, 6:1933-1941.