Detail

UniProtKB-Q13751


Protein	Laminin subunit beta-3
Gene	LAMB3
Status	Reviewed
Source	Breast Cancer cell lines; Breast cancer xenografts; Breast cell lines; Colorectal cancer cell line, HCT-116 (colorectal cancer); Hela cell line (cervical cancer); LNCap/PC3 cell lines (Prostate cancer); Non-small Cell Lung Carcinoma; OVCAR-3 cell line (Ovarian cancer); Ovarian tumor
Years	2011-2015

Glycosites

Site	Identified Peptides	Year(Publication ID)
220	ITNIRVnFTRIAP	2013(37);
220	IQEVGEITNLRVnFTR	2014(18); 2015(2);
604	RFGRIRnATASIW	2013(37);
604	nATASLWSGPGLEDR	2012(52); 2015(unpublished);2015(2); 2015(unpublished);2015(12);
604	LRnATASLWSGPGLEDR	2011(58); 2012(52); 2014(18); 2015(unpublished);2015(2); 2015(12);
810	nGTACGSR	2015(1);
810	EICPQDnGTACGS	2013(37);
810	QMACTPISCPGELCPQDnGTACGSR	2012(52); 2014(18); 2015(12);

Sequence

1	11	21	31	41	51	61	71	81	91
MRPFFLLCFA	LPGLLHAQQA	CSRGACYPPV	GDLLVGRTRF	LRASSTCGLT	KPETYCTQYG	EWQMKCCKCD	SRQPHNYYSH	RVENVASSSG	PMRWWQSQND
101	111	121	131	141	151	161	171	181	191
VNPVSLQLDL	DRRFQLQEVM	MEFQGPMPAG	MLIERSSDFG	KTWRVYQYLA	ADCTSTFPRV	RQGRPQSWQD	VRCQSLPQRP	NARLNGGKVQ	LNLMDLVSGI
201	211	221	231	241	251	261	271	281	291
PATQSQKIQE	VGEITNLRVN	FTRLAPVPQR	GYHPPSAYYA	VSQLRLQGSC	FCHGHADRCA	PKPGASAGPS	TAVQVHDVCV	CQHNTAGPNC	ERCAPFYNNR
301	311	321	331	341	351	361	371	381	391
PWRPAEGQDA	HECQRCDCNG	HSETCHFDPA	VFAASQGAYG	GVCDNCRDHT	EGKNCERCQL	HYFRNRRPGA	SIQETCISCE	CDPDGAVPGA	PCDPVTGQCV
401	411	421	431	441	451	461	471	481	491
CKEHVQGERC	DLCKPGFTGL	TYANPQGCHR	CDCNILGSRR	DMPCDEESGR	CLCLPNVVGP	KCDQCAPYHW	KLASGQGCEP	CACDPHNSLS	PQCNQFTGQC
501	511	521	531	541	551	561	571	581	591
PCREGFGGLM	CSAAAIRQCP	DRTYGDVATG	CRACDCDFRG	TEGPGCDKAS	GRCLCRPGLT	GPRCDQCQRG	YCNRYPVCVA	CHPCFQTYDA	DLREQALRFG
601	611	621	631	641	651	661	671	681	691
RLRNATASLW	SGPGLEDRGL	ASRILDAKSK	IEQIRAVLSS	PAVTEQEVAQ	VASAILSLRR	TLQGLQLDLP	LEEETLSLPR	DLESLDRSFN	GLLTMYQRKR
701	711	721	731	741	751	761	771	781	791
EQFEKISSAD	PSGAFRMLST	AYEQSAQAAQ	QVSDSSRLLD	QLRDSRREAE	RLVRQAGGGG	GTGSPKLVAL	RLEMSSLPDL	TPTFNKLCGN	SRQMACTPIS
801	811	821	831	841	851	861	871	881	891
CPGELCPQDN	GTACGSRCRG	VLPRAGGAFL	MAGQVAEQLR	GFNAQLQRTR	QMIRAAEESA	SQIQSSAQRL	ETQVSASRSQ	MEEDVRRTRL	LIQQVRDFLT
901	911	921	931	941	951	961	971	981	991
DPDTDAATIQ	EVSEAVLALW	LPTDSATVLQ	KMNEIQAIAA	RLPNVDLVLS	QTKQDIARAR	RLQAEAEEAR	SRAHAVEGQV	EDVVGNLRQG	TVALQEAQDT
1001	1011	1021	1031	1041	1051	1061	1071	1081	1091
MQGTSRSLRL	IQDRVAEVQQ	VLRPAEKLVT	SMTKQLGDFW	TRMEELRHQA	RQQGAEAVQA	QQLAEGASEQ	ALSAQEGFER	IKQKYAELKD	RLGQSSMLGE
1101	1111	1121	1131	1141	1151	1161	1171
QGARIQSVKT	EAEELFGETM	EMMDRMKDME	LELLRGSQAI	MLRSADLTGL	EKRVEQIRDH	INGRVLYYAT	CK

Reference

ID	Publication
1	Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2	Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
12	Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
18	Hirao Y, Matsuzaki H, Iwaki J, Kuno A, Kaji H, Ohkura T, Togayachi A, Abe M, Nomura M, Noguchi M, et al: Glycoproteomics Approach for Identifying Glycobiomarker Candidate Molecules for Tissue Type Classification of Non-small Cell Lung Carcinoma. Journal of Proteome Research 2014, 13:4705-4716.
37	Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
52	Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667.
58	Nagano K, Shinkawa T, Kato K, Inomata N, Yabuki N, Haramura M: Distinct cell surface proteome profiling by biotin labeling and glycoprotein capturing. Journal of Proteomics 2011, 74:1985-1993.