Contains      

UniProtKB-Q14108

Protein Lysosome membrane protein 2
Gene SCARB2
Status Reviewed
Source 22Rv1 cell xenograft (prostate cancer); ARO Cell Line (Thyroid Cance); Breast cancer cell lines; Breast cancer xenografts; Breast cancer xenograftsPBMC Macrophage cells; Breast cell lines; Breast tumor; Colorectal cancer cell line, HCT-116 (colorectal cancer); Colorectal tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); HCC cell lines (Liver); HEK293 cell line (embryonic kidney); HEK293T cell line (embryonic kidney); HeLa cell line (Cervical cancer); Hela cell line (cervical cancer); IGROV-1/CP cell line (Ovarian cancer)IGROV-1/CP; K562A cell line (Leukemia); K562S cell line (Leukemia); LNCap/PC3 cell lines (Prostate cancer); Liver; Liver tumor (HCC); OVCAR-3 cell line (Ovarian cancer); Ovarian tumor; PBMC Macrophage cells; Pancreatic islets; Platelet; Platelet Plasma membranes; Prostate; Prostate cancer cell lines; Prostate tumor; SKOV-3 cell line (Ovarian cancer); Serum; SpermatozoaJurkat T cell line; TPC-1 Cell Line (Thyroid Cance); Urine; ovarian tumor; prostate cancer cell lines
Years 2007-2015

Glycosites

Site Identified Peptides Year(Publication ID)
45 nGTEAFDSW 2014(33);
45 VLRnGTEAF 2014(33);
45 IVLRnGTEAF 2014(33);
45 RnGTEAFDSW 2014(33);
45 IVLRnGTEAFD 2014(14); 2015(unpublished);
45 KIVLRnGTEAF 2014(33);
45 VLRnGTEAFDSW 2014(33);
45 IVLRnGTEAFDSW 2014(33);
45 KIVLRnGTEAFDSW 2014(33);
45 IVLRnGTEAFDSWEK 2011(57);
45 nGTEAFDSWEKPPLPVY 2007(75); 2014(33);
45 RnGTEAFDSWEKPPLPVY 2014(33);
45 nGTEAFDSWEKPPLPVYTQF 2011(57); 2014(33);
45 IVLRnGTEAFDSWEKPPLPVY 2007(75); 2014(33);
45 nGTEAFDSWEKPPLPVYTQFY 2014(33);
45 RnGTEAFDSWEKPPLPVYTQFY 2014(33);
45 IVLRnGTEAFDSWEKPPLPVYTQF 2011(57);
45 nGTEAFDSWEKPPLPVYTQFYFFNVTNPEEILR 2009(64); 2011(58); 2012(52); 2014(15); 2014(33); 2015(unpublished);2015(2);
45 IVLRnGTEAFDSWEKPPLPVYTQFYFFNVTNPEEILR 2015(2);
68 FFnVTNPE 2014(33);
68 FFnVTNPEE 2014(33);
68 nVTNPEEIL 2014(33);
68 nVTNPEEILR 2007(75); 2014(14); 2014(33); 2015(1); 2015(unpublished);2007(unpublished);2007(unpublished);2007;
68 YFFnVTNPEE 2014(33);
68 FFnVTNPEEIL 2014(33);
68 FnVTNPEEILR 2007(75); 2012(48); 2014(14); 2014(33);
68 FYFFnVTNPEE 2014(33);
68 FFnVTNPEEILR 2007(75); 2012(48); 2014(14); 2014(19); 2014(33); 2015(unpublished);2015(unpublished);
68 YFFnVTNPEEIL 2014(33);
68 YFFnVTNPEEILR 2007(unpublished);2007(75); 2011(57); 2012(48); 2014(14); 2014(33); 2015(unpublished);
68 FFnVTNPEEILRGE 2014(33);
68 FYFFnVTNPEEILR 2014(33); 2015(unpublished);
68 YFFnVTNPEEILRGE 2014(33);
68 TQFYFFnVTNPEEILR 2007(75); 2014(33); 2015(unpublished);
68 FFnVTNPEEILRGETPR 2011(57); 2014(33);
68 YTQFYFFnVTNPEEILR 2015(unpublished);
68 VYTQFYFFnVTNPEEILR 2014(33);
68 YFFnVTNPEEILRGETPR 2011(57); 2014(33);
68 FFnVTNPEEILRGETPRVE 2014(33);
68 nVTNPEEILRGETPRVEEVGPY 2014(33);
68 FFnVTNPEEILRGETPRVEEVGP 2014(33);
68 KPPLPVYTQFYFFnVTNPEEILR 2014(33);
68 FFnVTNPEEILRGETPRVEEVGPY 2014(33);
68 YFFnVTNPEEILRGETPRVEEVGP 2014(33);
68 YFFnVTNPEEILRGETPRVEEVGPY 2014(33);
68 SWEKPPLPVYTQFYFFnVTNPEEILR 2014(33);
68 NGTEAFDSWEKPPLPVYTQFYFFnVTNPEEILR 2009(64); 2011(58); 2012(52); 2014(15); 2014(33); 2015(unpublished);2015(2);
68 IVLRNGTEAFDSWEKPPLPVYTQFYFFnVTNPEEILR 2015(2);
105 nGTTISAVSNK 2014(14); 2015(1);
105 NIQFGDnGTTI 2014(33);
105 ANIQFGDnGTTI 2014(14); 2014(33); 2015(unpublished);
105 ANIQFGDnGTTIS 2014(33);
105 GDnGTTISAVSNK 2014(14); 2014(33);
105 KANIQFGDnGTTI 2014(33);
105 NKANIQFGDnGTTI 2014(33);
105 ANIQFGDnGTTISAV 2014(14); 2015(unpublished);
105 GDnGTTISAVSNKAY 2014(33);
105 RNKANIQFGDnGTTI 2014(33);
105 IQFGDnGTTISAVSNK 2012(48); 2012(52); 2014(14); 2014(33); 2015(unpublished);
105 ANIQFGDnGTTISAVSNK 2007(72); 2007(74); 2007(75); 2007(62); 2009(62); 2009(64); 2009(54); 2011(57); 2011(58); 2011(48); 2012(50); 2012(52); 2012(34); 2013(34); 2013(34); 2013(40); 2013(43); 2013(13); 2014(14); 2014(15); 2014(17); 2014(19); 2014(22); 2014(25); 2014(25); 2014(32); 2014(32); 2014(33); 2014(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015;2015;2015;2007;2007;2007;2007;2007;
105 RNKANIQFGDnGTTISAV 2014(33);
105 ANIQFGDnGTTISAVSNKAY 2014(33);
105 NKANIQFGDnGTTISAVSNK 2007(72); 2007(75); 2011(58); 2012(52); 2013(34); 2013(34); 2014(14); 2014(15); 2014(16); 2014(33); 2015(1); 2015(unpublished);2015(2); 2015(unpublished);2015(unpublished);2015(12);
105 RNKANIQFGDnGTTISAVSN 2014(33);
105 NKANIQFGDnGTTISAVSNKAY 2014(33);
105 ELRNKANIQFGDnGTTISAVSNK 2012(52);
105 RNKANIQFGDnGTTISAVSNKAY 2014(33);
206 EKnGTNDGDY 2014(33);
206 YEKnGTNDGDY 2014(33);
206 FYEKnGTNDGDY 2014(33);
206 nGTNDGDYVFLT 2014(33);
206 KnGTNDGDYVFLT 2014(33);
206 LFYEKnGTNDGDY 2009(64);
206 YEKnGTNDGDYVF 2014(33);
206 nGTNDGDYVFLTGE 2014(33);
206 YEKnGTNDGDYVFL 2014(33);
206 FGLFYEKnGTNDGDY 2014(33);
206 FYEKnGTNDGDYVFL 2014(33);
206 KnGTNDGDYVFLTGE 2014(33);
206 FGLFYEKnGTNDGDYV 2014(33);
206 FGLFYEKnGTNDGDYVF 2014(33);
206 YEKnGTNDGDYVFLTGE 2014(33);
206 FGLFYEKnGTNDGDYVFL 2014(33);
206 EKnGTNDGDYVFLTGEDSYL 2014(33);
206 YEKnGTNDGDYVFLTGEDSY 2014(33);
206 YEKnGTNDGDYVFLTGEDSYL 2014(33);
206 nGTNDGDYVFITGEDSYINFTK 2014(32);
206 nGTNDGDYVFLTGEDSYLNFTK 2007(72); 2009(67); 2011(57); 2012(52); 2014(14); 2014(25); 2014(25); 2014(33); 2015(1); 2015(2); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2007;2007;2007;2007;2007;
206 KnGTNDGDYVFLTGEDSYLNFTK 2014(33);
206 YEKnGTNDGDYVFLTGEDSYLNF 2014(33);
206 YEKnGTNDGDYVFLTGEDSYLNFTK 2014(33);
206 FYEKnGTNDGDYVFLTGEDSYLNFTK 2014(33);
224 YLnFTK 2014(33); 2015(unpublished);2015(unpublished);
224 DSYLnFTK 2014(33);
224 nFTKIVEW 2014(33);
224 TGEDSYLnF 2014(33);
224 GEDSYLnFTK 2012(48); 2014(14); 2014(33); 2015(unpublished);2015(unpublished);
224 LTGEDSYLnF 2014(33);
224 DSYLnFTKIVE 2014(33);
224 TGEDSYLnFTK 2007(75); 2014(14); 2014(33); 2015(unpublished);

Sequence

1112131415161718191
MGRCCFYTAGTLSLLLLVTSVTLLVARVFQKAVDQSIEKKIVLRNGTEAFDSWEKPPLPVYTQFYFFNVTNPEEILRGETPRVEEVGPYTYRELRNKANI
101111121131141151161171181191
QFGDNGTTISAVSNKAYVFERDQSVGDPKIDLIRTLNIPVLTVIEWSQVHFLREIIEAMLKAYQQKLFVTHTVDELLWGYKDEILSLIHVFRPDISPYFG
201211221231241251261271281291
LFYEKNGTNDGDYVFLTGEDSYLNFTKIVEWNGKTSLDWWITDKCNMINGTDGDSFHPLITKDEVLYVFPSDFCRSVYITFSDYESVQGLPAFRYKVPAE
301311321331341351361371381391
ILANTSDNAGFCIPEGNCLGSGVLNVSICKNGAPIIMSFPHFYQADERFVSAIEGMHPNQEDHETFVDINPLTGIILKAAKRFQINIYVKKLDDFVETGD
401411421431441451461471
IRTMVFPVMYLNESVHIDKETASRLKSMINTTLIITNIPYIIMALGVFFGLVFTWLACKGQGSMDEGTADERAPLIRT

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
12Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
15Chen R, Seebun D, Ye M, Zou H, Figeys D: Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS. Journal of Proteomics 2014, 103:194-203.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
17Fang C, Xiong Z, Qin H, Huang G, Liu J, Ye M, Feng S, Zou H: One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides. Analytica Chimica Acta 2014, 841:99-105.
19Huang G, Sun Z, Qin H, Zhao L, Xiong Z, Peng X, Ou J, Zou H: Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides. Analyst 2014, 139:2199-2206.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
25Sun Z, Dong J, Zhang S, Hu Z, Cheng K, Li K, Xu B, Ye M, Nie Y, Fan D, Zou H: Identification of Chemoresistance-Related Cell-Surface Glycoproteins in Leukemia Cells and Functional Validation of Candidate Glycoproteins. Journal of Proteome Research 2014, 13:1593-1601.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
43Wang GG, Wu YB, Zhou T, Guo YS, Zheng B, Wang J, Bi Y, Liu FJ, Zhou ZM, Guo XJ, Sha JH: Mapping of the N-Linked Glycoproteome of Human Spermatozoa. Journal of Proteome Research 2013, 12:5750-5759.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
50Whitmore TE, Peterson A, Holzman T, Eastham A, Amon L, McIntosh M, Ozinsky A, Nelson PS, Martin DB: Integrative Analysis of N-Linked Human Glycoproteomic Data Sets Reveals PTPRF Ectodomain as a Novel Plasma Biomarker Candidate for Prostate Cancer. Journal of Proteome Research 2012, 11:2653-2665.
52Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667.
54Tian Y, Yao Z, Roden R, Zhang H: Identification of glycoproteins associated with different histological subtypes of ovarian tumors using quantitative glycoproteomics. Proteomics 2011, 11:4677-4687.
57Chen Y, Cao J, Yan G, Lu H, Yang P: Two-step protease digestion and glycopeptide capture approach for accurate glycosite identification and glycoprotein sequence coverage improvement. Talanta 2011, 85:70-75.
58Nagano K, Shinkawa T, Kato K, Inomata N, Yabuki N, Haramura M: Distinct cell surface proteome profiling by biotin labeling and glycoprotein capturing. Journal of Proteomics 2011, 74:1985-1993.
62Arcinas A, Yen T-Y, Kebebew E, Macher BA: Cell Surface and Secreted Protein Profiles of Human Thyroid Cancer Cell Lines Reveal Distinct Glycoprotein Patterns. Journal of Proteome Research 2009, 8:3958-3968.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
67McDonald CA, Yang JY, Marathe V, Yen T-Y, Macher BA: Combining Results from Lectin Affinity Chromatography and Glycocapture Approaches Substantially Improves the Coverage of the Glycoproteome. Molecular & Cellular Proteomics 2009, 8:287-301.
72Hanson SR, Hsu T-L, Weerapana E, Kishikawa K, Simon GM, Cravatt BF, Wong C-H: Tailored glycoproteomics and glycan site mapping using saccharide-selective bioorthogonal probes. Journal of the American Chemical Society 2007, 129:7266-+.
74Lewandrowski U, Zahedi RP, Moebius J, Walter U, Sickmann A: Enhanced N-glycosylation site analysis of Sialoglycopeptides by strong cation exchange prefractionation applied to platelet plasma membranes. Molecular & Cellular Proteomics 2007, 6:1933-1941.
75Sun B, Ranish JA, Utleg AG, White JT, Yan X, Lin B, Hood L: Shotgun glycopeptide capture approach coupled with mass Spectrometry for comprehensive glycoproteomics. Molecular & Cellular Proteomics 2007, 6:141-149.