Contains      

UniProtKB-Q16363

Protein Laminin subunit alpha-4
Gene LAMA4
Status Reviewed
Source 22Rv1 cell xenograft (prostate cancer); Bladder tumor; Breast Cancer cell lines; Breast cancer xenografts; Breast tumor; Colorectal tumor; DRO-1 Cell Line (Thyroid Cance); HCC cell lines (Liver); HCCLM3 cell line (Liver); HEK293T cell line (embryonic kidney); Hela cell line (cervical cancer); Liver; Liver tumor (HCC); Ovarian tumor; Platelet; Prostate; Prostate cancer metastasis to liver; Prostate stromal cell line; Prostate tumor; Serum; Urine; ovarian tumor
Years 2007-20072007

Glycosites

Site Identified Peptides Year(Publication ID)
104 nTTGEHCEK 2015(unpublished);2015(unpublished);
215 nTTGFK 2015(unpublished);2015(unpublished);
315 InATIYLLK 2014(33);
315 HVNEInATIY 2014(33);
315 HVNEInATIYLLK 2007(unpublished);2014(33); 2015(unpublished);2015(unpublished);
315 RHVNEInATIYII 2013(37);
465 LHnETR 2013(40);
531 VVnMSLSTSADSLTTPR 2014(33);
531 EQMEVVnMSLSTSADSLTTPR 2009(62); 2011(54); 2013(34); 2013(40); 2014(14); 2014(22); 2014(33); 2015(unpublished);2015(unpublished);2015(unpublished);2015(Unpublished ); 2015;2015;2007;2007;2007;2007 ;
531 VREQMEVVnMSLSTSADSLTTPR 2015(unpublished);2015(unpublished);2015(unpublished);
557 IDDIIKnASGIYA 2013(37);
557 nASGIYAEIDGAK 2009(63); 2013(40); 2014(22); 2014(33); 2015(12); 2015(unpublished);2015(unpublished);2007;2007;
557 LDDIIKnASGIYAE 2014(33);
557 LTLSELDDIIKnASGIYAEIDGAK 2009(64); 2014(33); 2015(unpublished);2015(unpublished);
578 IQVKISnISNISH 2013(37);
578 LSnLSNLSHDLVQE 2014(33);
578 ISnISNISHDIVQEAIDHAQDIQQEANEISR 2014(32);
578 LSnLSNLSHDLVQEAIDHAQDLQQEANELSR 2009(64); 2013(40); 2014(33); 2015(12); 2015(unpublished);2015(UnpublishedUnpublished ); 2007;20072007 ;
581 IQVKISNISnISH 2013(37);
581 LSNLSnLSHDLVQE 2014(33);
581 ISNISnISHDIVQEAIDHAQDIQQEANEISR 2014(32);
581 LSNLSnLSHDLVQEAIDHAQDLQQEANELSR 2009(64); 2013(40); 2014(33); 2015(12); 2015(unpublished);2015(UnpublishedUnpublished ); 2007;20072007 ;
638 VSEAnETAEF 2014(33);
638 VSEAnETAEFALNTTDR 2015(unpublished);
638 ALDASNVYENIVNYVSEAnETAEFALNTTDR 2007(unpublished);2007(unpublished);20072007 (UnpublishedUnpublished );
646 FALnTTDR 2014(33);
646 ALnTTDRIY 2014(33);
646 VSEANETAEFALnTTDR 2015(unpublished);
646 ALDASNVYENIVNYVSEANETAEFALnTTDR 2007(unpublished);2007(unpublished);20072007 (UnpublishedUnpublished );
742 LITEEAnR 2013(40); 2014(14); 2014(22); 2014(33); 2015(unpublished);2015(unpublished);2015(unpublished);2015;2007;2007;2007;
742 ITEEAnRTTME 2009(64);
742 IITEEAnRTTMEV 2013(37);
787 ARDAVRnITEVVP 2013(37);
787 nLTEVVPQLLDQLR 2009(63); 2009(64); 2011(57); 2012(50); 2013(40); 2014(33); 2015(12); 2015(unpublished);2015(unpublished);2015(UnpublishedUnpublished ); 2007;2007;20072007 ;
787 DAVRnLTEVVPQLLDQLR 2015(unpublished);2015(12);
810 ASnVSASIQR 2014(14); 2015(unpublished);2015(unpublished);
810 PASnVSASIQR 2014(33);
810 RPASnVSASIQR 2009(64); 2009(65); 2011(57); 2012(3); 2013(34); 2013(40); 2014(14); 2014(19); 2014(22); 2014(32); 2014(32); 2014(33); 2015(12); 2015(unpublished);2015(unpublished);2015;2015;2015;2015;2007;2007;
810 QKRPASnVSASIQ 2013(37);
1093 LMVnGSMFF 2014(33);
1093 TPADNGLILLMVnGSMFFR 2014(33); 2015(unpublished);2007(UnpublishedUnpublished ); 20072007 ;
1288 SLDnGTVIMDVK 2007(unpublished);2007 (Unpublished );
1288 SISLDnGTVIMDVK 2014(33);
1366 AnFTGCISNAY 2014(33);
1366 FGGSPISAQYAnF 2014(33);
1366 FYFGGSPISAQYAnF 2014(33);
1366 KFYFGGSPISAQYAnF 2014(33);
1366 SAQYAnFTGCISNAYFTR 2007(unpublished);2007(unpublished);20072007 (UnpublishedUnpublished );
1366 FYFGGSPISAQYAnFTGCISNAYFTR 2015(unpublished);
1366 KFYFGGSPISAQYAnFTGCISNAYFTR 2014(33);

Sequence

1112131415161718191
MALSSAWRSVLPLWLLWSAACSRAASGDDNAFPFDIEGSSAVGRQDPPETSEPRVALGRLPPAAEKCNAGFFHTLSGECVPCDCNGNSNECLDGSGYCVH
101111121131141151161171181191
CQRNTTGEHCEKCLDGYIGDSIRGAPQFCQPCPCPLPHLANFAESCYRKNGAVRCICNENYAGPNCERCAPGYYGNPLLIGSTCKKCDCSGNSDPNLIFE
201211221231241251261271281291
DCDEVTGQCRNCLRNTTGFKCERCAPGYYGDARIAKNCAVCNCGGGPCDSVTGECLEEGFEPPTGMDCPTISCDKCVWDLTDALRLAALSIEEGKSGVLS
301311321331341351361371381391
VSSGAAAHRHVNEINATIYLLKTKLSERENQYALRKIQINNAENTMKSLLSDVEELVEKENQASRKGQLVQKESMDTINHASQLVEQAHDMRDKIQEINN
401411421431441451461471481491
KMLYYGEEHELSPKEISEKLVLAQKMLEEIRSRQPFFTQRELVDEEADEAYELLSQAESWQRLHNETRTLFPVVLEQLDDYNAKLSDLQEALDQALNYVR
501511521531541551561571581591
DAEDMNRATAARQRDHEKQQERVREQMEVVNMSLSTSADSLTTPRLTLSELDDIIKNASGIYAEIDGAKSELQVKLSNLSNLSHDLVQEAIDHAQDLQQE
601611621631641651661671681691
ANELSRKLHSSDMNGLVQKALDASNVYENIVNYVSEANETAEFALNTTDRIYDAVSGIDTQIIYHKDESENLLNQARELQAKAESSSDEAVADTSRRVGG
701711721731741751761771781791
ALARKSALKTRLSDAVKQLQAAERGDAQQRLGQSRLITEEANRTTMEVQQATAPMANNLTNWSQNLQHFDSSAYNTAVNSARDAVRNLTEVVPQLLDQLR
801811821831841851861871881891
TVEQKRPASNVSASIQRIRELIAQTRSVASKIQVSMMFDGQSAVEVHSRTSMDDLKAFTSLSLYMKPPVKRPELTETADQFILYLGSKNAKKEYMGLAIK
901911921931941951961971981991
NDNLVYVYNLGTKDVEIPLDSKPVSSWPAYFSIVKIERVGKHGKVFLTVPSLSSTAEEKFIKKGEFSGDDSLLDLDPEDTVFYVGGVPSNFKLPTSLNLP
1001101110211031104110511061107110811091
GFVGCLELATLNNDVISLYNFKHIYNMDPSTSVPCARDKLAFTQSRAASYFFDGSGYAVVRDITRRGKFGQVTRFDIEVRTPADNGLILLMVNGSMFFRL
1101111111211131114111511161117111811191
EMRNGYLHVFYDFGFSGGPVHLEDTLKKAQINDAKYHEISIIYHNDKKMILVVDRRHVKSMDNEKMKIPFTDIYIGGAPPEILQSRALRAHLPLDINFRG
1201121112211231124112511261127112811291
CMKGFQFQKKDFNLLEQTETLGVGYGCPEDSLISRRAYFNGQSFIASIQKISFFDGFEGGFNFRTLQPNGLLFYYASGSDVFSISLDNGTVIMDVKGIKV
1301131113211331134113511361137113811391
QSVDKQYNDGLSHFVISSVSPTRYELIVDKSRVGSKNPTKGKIEQTQASEKKFYFGGSPISAQYANFTGCISNAYFTRVDRDVEVEDFQRYTEKVHTSLY
1401141114211431144114511461147114811491
ECPIESSPLFLLHKKGKNLSKPKASQNKKGGKSKDAPSWDPVALKLPERNTPRNSHCHLSNSPRAIEHAYQYGGTANSRQEFEHLKGDFGAKSQFSIRLR
1501151115211531154115511561157115811591
TRSSHGMIFYVSDQEENDFMTLFLAHGRLVYMFNVGHKKLKIRSQEKYNDGLWHDVIFIRERSSGRLVIDGLRVLEESLPPTEATWKIKGPIYLGGVAPG
1601161116211631164116511661167116811691
KAVKNVQINSIYSFSGCLSNLQLNGASITSASQTFSVTPCFEGPMETGTYFSTEGGYVVLDESFNIGLKFEIAFEVRPRSSSGTLVHGHSVNGEYLNVHM
1701171117211731174117511761177117811791
KNGQVIVKVNNGIRDFSTSVTPKQSLCDGRWHRITVIRDSNVVQLDVDSEVNHVVGPLNPKPIDHREPVFVGGVPESLLTPRLAPSKPFTGCIRHFVIDG
180118111821
HPVSFSKAALVSGAVSINSCPAA

Reference

ID Publication
3Tian Y, Almaraz RT, Choi CH, Li QK, Saeui C, Li D, Shah P, Bhattacharya R, Yarema KJ, Zhang H: Identification of sialylated glycoproteins from metabolically oligosaccharide engineered pancreatic cells. Clinical proteomics 2015, 12:11.
12Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
19Huang G, Sun Z, Qin H, Zhao L, Xiong Z, Peng X, Ou J, Zou H: Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides. Analyst 2014, 139:2199-2206.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
50Whitmore TE, Peterson A, Holzman T, Eastham A, Amon L, McIntosh M, Ozinsky A, Nelson PS, Martin DB: Integrative Analysis of N-Linked Human Glycoproteomic Data Sets Reveals PTPRF Ectodomain as a Novel Plasma Biomarker Candidate for Prostate Cancer. Journal of Proteome Research 2012, 11:2653-2665.
54Tian Y, Yao Z, Roden R, Zhang H: Identification of glycoproteins associated with different histological subtypes of ovarian tumors using quantitative glycoproteomics. Proteomics 2011, 11:4677-4687.
57Chen Y, Cao J, Yan G, Lu H, Yang P: Two-step protease digestion and glycopeptide capture approach for accurate glycosite identification and glycoprotein sequence coverage improvement. Talanta 2011, 85:70-75.
62Arcinas A, Yen T-Y, Kebebew E, Macher BA: Cell Surface and Secreted Protein Profiles of Human Thyroid Cancer Cell Lines Reveal Distinct Glycoprotein Patterns. Journal of Proteome Research 2009, 8:3958-3968.
63Cao J, Shen C, Wang H, Shen H, Chen Y, Nie A, Yan G, Lu H, Liu Y, Yang P: Identification of N-Glycosylation Sites on Secreted Proteins of Human Hepatocellular Carcinoma Cells with a Complementary Proteomics Approach. Journal of Proteome Research 2009, 8:662-672.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
65Goo YA, Lilu AY, Ryu S, Shaffer SA, Malmstrom L, Page L, Nguyen LT, Doneanu CE, Goodlett DR: Identification of Secreted Glycoproteins of Human Prostate and Bladder Stromal Cells by Comparative Quantitative Proteomics. Prostate 2009, 69:49-61.