Contains      

UniProtKB-Q3T906

Protein N-acetylglucosamine-1-phosphotransferase subunits alpha/beta
Gene GNPTAB
Status Reviewed
Source Breast cancer xenografts; Breast cell lines; Breast cell linesJurkat T cell line; Colorectal cancer cell line, HCT-116 (colorectal cancer); Colorectal tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); HEK293 cell line (embryonic kidney); Hela cell line (cervical cancer); Jurkat T cell line; LNCap/PC3 cell lines (Prostate cancer); Liver; OVCAR-3 cell line (Ovarian cancer); Ovarian tumor; Pancreatic islets; Prostate; Prostate tumor; Serum; Urine
Years 2007-2015

Glycosites

Site Identified Peptides Year(Publication ID)
83 DVVYTWVnGTDLELLK 2012(48);
114 EILGKnTTEPTKK 2014(13);
148 VPMIVIDPAIPAnITIK 2014(16); 2014(32);
148 VPMLVLDPALPAnITLK 2011(58); 2012(48); 2012(52); 2014(13); 2014(14); 2014(22); 2014(33); 2015(unpublished);2015(1); 2015(2); 2015(unpublished);
179 NPSTnVSVVVFDSTK 2014(13); 2014(33); 2015(unpublished);2015(1); 2015(2); 2015(unpublished);
179 NPSTnVSVVVFDSTKDVEDAHSGIIK 2014(16);
179 NPSTnVSVVVFDSTKDVEDAHSGLLK 2015(1);
287 nMTIDGK 2015(unpublished);2015(unpublished);
287 KnMTIDGK 2015(unpublished);2015(2);
287 QTKKnMTIDGK 2014(16);
287 LNNPKDFQELNKQTKKnMTIDGK 2007(unpublished);2007(unpublished);
376 nLSHLPTFSSPAIE 2014(33);
376 nLSHLPTFSSPAIESHIHR 2015(unpublished);2015(2); 2015(12);
457 ACnNSACDWDGGDCSGNSGGSR 2014(16); 2015(unpublished);
551 nQTHYIIPK 2015(1);
551 KVILLPnQTHY 2014(33);
551 VIIIPnQTHYIIPK 2014(16);
551 VILLPnQTHYIIPK 2014(14); 2014(33); 2015(unpublished);2015(1); 2015(2); 2015(unpublished);
607 TIHLIMHSGMnATTIHFNLTFQNTNDEEFK 2015(unpublished);
614 TIHLIMHSGMNATTIHFnLTFQNTNDEEFK 2015(unpublished);
642 LnSTAQK 2015(unpublished);2015(unpublished);
642 EGPKInSTAQK 2014(16);
642 EGPKLnSTAQK 2015(unpublished);2015(2);
684 HDVnSTR 2015(unpublished);
699 nISLLPK 2015(1);
699 VnISLLPK 2012(48);
699 EVKIPLVnISL 2009(64);
699 IPLVnISLLPK 2011(58); 2012(52); 2014(13); 2014(14); 2014(33); 2015(unpublished);2015(1); 2015(2); 2015(unpublished);
699 AQEEVKIPLVnISLLPK 2014(14); 2014(15); 2015(1); 2015(2); 2015(unpublished);
699 RAQEEVKIPIVnISIIPK 2014(16);
729 GYnLSK 2015(2); 2015(unpublished);
759 nDSLVAPQEK 2015(1);
759 NQAIITDETnDSLVAPQEK 2012(48); 2012(52); 2014(13); 2014(14); 2014(22); 2014(33); 2015(1); 2015(2); 2015(unpublished);2015(unpublished);2007;2007;
759 IKNQAIITDETnDSIVAPQEK 2014(16);
759 IKNQAIITDETnDSLVAPQEK 2014(13); 2015(2); 2015(unpublished);2015(unpublished);
829 TIGGnVTK 2015(unpublished);
829 TIGGnVTKEKPPSLIVPLESQMT 2007(unpublished);2007(unpublished);2007;
1009 SAVQPLnISQVFDEVDTDQSGVLSDR 2015(unpublished);
1056 QDLTGLEHMLInCSK 2012(48);
1056 IHEIPISIQDITGIEHMIInCSK 2014(16);
1056 IHELPLSLQDLTGLEHMLInCSK 2014(33); 2015(unpublished);2015(12);
1129 TnVSHVVGQLDDIR 2014(13); 2014(33); 2015(unpublished);2015(2); 2015(unpublished);2015(12);
1129 TnVSHVVGQIDDIRK 2014(16);
1129 TnVSHVVGQLDDIRK 2014(13); 2015(1); 2015(2); 2015(unpublished);

Sequence

1112131415161718191
MLFKLLQRQTYTCLSHRYGLYVCFLGVVVTIVSAFQFGEVVLEWSRDQYHVLFDSYRDNIAGKSFQNRLCLPMPIDVVYTWVNGTDLELLKELQQVREQM
101111121131141151161171181191
EEEQKAMREILGKNTTEPTKKSEKQLECLLTHCIKVPMLVLDPALPANITLKDLPSLYPSFHSASDIFNVAKPKNPSTNVSVVVFDSTKDVEDAHSGLLK
201211221231241251261271281291
GNSRQTVWRGYLTTDKEVPGLVLMQDLAFLSGFPPTFKETNQLKTKLPENLSSKVKLLQLYSEASVALLKLNNPKDFQELNKQTKKNMTIDGKELTISPA
301311321331341351361371381391
YLLWDLSAISQSKQDEDISASRFEDNEELRYSLRSIERHAPWVRNIFIVTNGQIPSWLNLDNPRVTIVTHQDVFRNLSHLPTFSSPAIESHIHRIEGLSQ
401411421431441451461471481491
KFIYLNDDVMFGKDVWPDDFYSHSKGQKVYLTWPVPNCAEGCPGSWIKDGYCDKACNNSACDWDGGDCSGNSGGSRYIAGGGGTGSIGVGQPWQFGGGIN
501511521531541551561571581591
SVSYCNQGCANSWLADKFCDQACNVLSCGFDAGDCGQDHFHELYKVILLPNQTHYIIPKGECLPYFSFAEVAKRGVEGAYSDNPIIRHASIANKWKTIHL
601611621631641651661671681691
IMHSGMNATTIHFNLTFQNTNDEEFKMQITVEVDTREGPKLNSTAQKGYENLVSPITLLPEAEILFEDIPKEKRFPKFKRHDVNSTRRAQEEVKIPLVNI
701711721731741751761771781791
SLLPKDAQLSLNTLDLQLEHGDITLKGYNLSKSALLRSFLMNSQHAKIKNQAIITDETNDSLVAPQEKQVHKSILPNSLGVSERLQRLTFPAVSVKVNGH
801811821831841851861871881891
DQGQNPPLDLETTARFRVETHTQKTIGGNVTKEKPPSLIVPLESQMTKEKKITGKEKENSRMEENAENHIGVTEVLLGRKLQHYTDSYLGFLPWEKKKYF
901911921931941951961971981991
QDLLDEEESLKTQLAYFTDSKNTGRQLKDTFADSLRYVNKILNSKFGFTSRKVPAHMPHMIDRIVMQELQDMFPEEFDKTSFHKVRHSEDMQFAFSYFYY
1001101110211031104110511061107110811091
LMSAVQPLNISQVFDEVDTDQSGVLSDREIRTLATRIHELPLSLQDLTGLEHMLINCSKMLPADITQLNNIPPTQESYYDPNLPPVTKSLVTNCKPVTDK
1101111111211131114111511161117111811191
IHKAYKDKNKYRFEIMGEEEIAFKMIRTNVSHVVGQLDDIRKNPRKFVCLNDNIDHNHKDAQTVKAVLRDFYESMFPIPSQFELPREYRNRFLHMHELQE
120112111221123112411251
WRAYRDKLKFWTHCVLATLIMFTIFSFFAEQLIALKRKIFPRRRIHKEASPNRIRV

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
12Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
15Chen R, Seebun D, Ye M, Zou H, Figeys D: Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS. Journal of Proteomics 2014, 103:194-203.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
52Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667.
58Nagano K, Shinkawa T, Kato K, Inomata N, Yabuki N, Haramura M: Distinct cell surface proteome profiling by biotin labeling and glycoprotein capturing. Journal of Proteomics 2011, 74:1985-1993.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.