Detail

UniProtKB-Q5VU97


Protein	VWFA and cache domain-containing protein 1
Gene	CACHD1
Status	Reviewed
Source	Breast Cancer cell lines; DLBCL cell lines (Diffuse large B-cell lymphoma); Liver; OVCAR-3 cell line (Ovarian cancer)
Years	2013-2015

Glycosites

Site	Identified Peptides	Year(Publication ID)
145	FDGNFNTnVSR	2014(33);
587	nVSYAWK	2015(1);
587	TGKEAYnVSYAWK	2013(37);
905	QRFYKFnTSIAGD	2013(37);
905	FnTSIAGDITNIVHGSHCSK	2014(16);
985	nLTNAENR	2015(1);

Sequence

1	11	21	31	41	51	61	71	81	91
MARQPEEEET	AVARARRPPL	WLLCLVACWL	LGAGAEADFS	ILDEAQVLAS	QMRRLAAEEL	GVVTMQRIFN	SFVYTEKISN	GESEVQQLAK	KIREKFNRYL
101	111	121	131	141	151	161	171	181	191
DVVNRNKQVV	EASYTAHLTS	PLTAIQDCCT	IPPSMMEFDG	NFNTNVSRTI	SCDRLSTTVN	SRAFNPGRDL	NSVLADNLKS	NPGIKWQYFS	SEEGIFTVFP
201	211	221	231	241	251	261	271	281	291
AHKFRCKGSY	EHRSRPIYVS	TVRPQSKHIV	VILDHGASVT	DTQLQIAKDA	AQVILSAIDE	HDKISVLTVA	DTVRTCSLDQ	CYKTFLSPAT	SETKRKMSTF
301	311	321	331	341	351	361	371	381	391
VSSVKSSDSP	TQHAVGFQKA	FQLIRSTNNN	TKFQANTDMV	IIYLSAGITS	KDSSEEDKKA	TLQVINEENS	FLNNSVMILT	YALMNDGVTG	LKELAFLRDL
401	411	421	431	441	451	461	471	481	491
AEQNSGKYGV	PDRMALPVIK	GSMMVLNQLS	NLETTVGRFY	TNLPNRMIDE	AVFSLPFSDE	MGDGLIMTVS	KPCYFGNLLL	GIVGVDVNLA	YILEDVTYYQ
501	511	521	531	541	551	561	571	581	591
DSLASYTFLI	DDKGYTLMHP	SLTRPYLLSE	PPLHTDIIHY	ENIPKFELVR	QNILSLPLGS	QIIAVPVNSS	LSWHINKLRE	TGKEAYNVSY	AWKMVQDTSF
601	611	621	631	641	651	661	671	681	691
ILCIVVIQPE	IPVKQLKNLN	TVPSSKLLYH	RLDLLGQPSA	CLHFKQLATL	ESPTIMLSAG	SFSSPYEHLS	QPETKRMVEH	YTAYLSDNTR	LIANPGLKFS
701	711	721	731	741	751	761	771	781	791
VRNEVMATSH	VTDEWMTQME	MSSLNTYIVR	RYIATPNGVL	RIYPGSLMDK	AFDPTRRQWY	LHAVANPGLI	SLTGPYLDVG	GAGYVVTISH	TIHSSSTQLS
801	811	821	831	841	851	861	871	881	891
SGHTVAVMGI	DFTLRYFYKV	LMDLLPVCNQ	DGGNKIRCFI	MEDRGYLVAH	PTLIDPKGHA	PVEQQHITHK	EPLVANDILN	HPNFVKKNLC	NSFSDRTVQR
901	911	921	931	941	951	961	971	981	991
FYKFNTSLAG	DLTNLVHGSH	CSKYRLARIP	GTNAFVGIVN	ETCDSLAFCA	CSMVDRLCLN	CHRMEQNECE	CPCECPLEVN	ECTGNLTNAE	NRNPSCEVHQ
1001	1011	1021	1031	1041	1051	1061	1071	1081	1091
EPVTYTAIDP	GLQDALHQCV	NSRCSQRLES	GDCFGVLDCE	WCMVDSDGKT	HLDKPYCAPQ	KECFGGIVGA	KSPYVDDMGA	IGDEVITLNM	IKSAPVGPVA
1101	1111	1121	1131	1141	1151	1161	1171	1181	1191
GGIMGCIMVL	VLAVYAYRHQ	IHRRSHQHMS	PLAAQEMSVR	MSNLENDRDE	RDDDSHEDRG	IISNTRFIAA	VIERHAHSPE	RRRRYWGRSG	TESDHGYSTM
1201	1211	1221	1231	1241	1251	1261	1271
SPQEDSENPP	CNNDPLSAGV	DVGNHDEDLD	LDTPPQTAAL	LSHKFHHYRS	HHPTLHHSHH	LQAAVTVHTV	DAEC

Reference

ID	Publication
1	Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
16	Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
33	Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
37	Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.