Contains      

UniProtKB-Q5ZPR3

Protein CD276 antigen
Gene CD276
Status Reviewed
Source 22Rv1 cell line (prostate cancer); 22Rv1 cell xenograft (prostate cancer); ARO Cell Line (Thyroid Cance); Bladder tumor; Breast Cancer cell lines; Breast cancer cell lines; Breast cancer xenografts; Breast cancer xenograftsPBMC Macrophage cells; Breast cell lines; Breast tumor; Colorectal cancer cell line, HCT-116 (colorectal cancer); Colorectal tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); DRO-1 Cell Line (Thyroid Cance); FTC-133 Cell Line (Thyroid Cance); HCC cell lines (Liver); HCCLM3 cell line (Liver); HEK293 cell line (embryonic kidney); HEK293T cell line (embryonic kidney); HEK293T cell line (embryonic kidney), HEK293T cell line (embryonic kidney); HeLa cell line (Cervical cancer); Hela cell line (cervical cancer); HuH-7 cell line (Liver); IGROV-1/CP cell line (Ovarian cancer)IGROV-1/CP; LNCap/PC3 cell lines (Prostate cancer); Liver; Liver tumor (HCC); LnCap cell line (Prostate cancer); Non-small Cell Lung Carcinoma; OVCAR-3 cell line (Ovarian cancer); Ovarian cancer cell lines; Ovarian tumor; Pancreatic islets; Plasma; Prostate; Prostate cancer cell lines; Prostate cancer metastasis to liver; Prostate tumor; SKOV-3 cell line (Ovarian cancer); Serum; TPC-1 Cell Line (Thyroid Cance); Urine; XTC-1 Cell Line (Thyroid Cance); prostate cancer cell lines
Years 2003-2015

Glycosites

Site Identified Peptides Year(Publication ID)
91 AnRTALF 2014(33);
91 DQGSAYAnR 2005( 81); 2012(50); 2013(34); 2013(34); 2013(34); 2013(40); 2013(45); 2014(14); 2014(15); 2014(22); 2014(33); 2015(2); 2015(10); 2015(unpublished);2015(unpublished);2015;2015;2015;2015;2007;2007;
91 AnRTALFPDLL 2014(33);
91 GQDQGSAYAnR 2014(33);
91 QGSAYAnRTAIFP 2013(37);
91 AnRTALFPDLLAQGNASL 2014(33);
91 QIVHSFAEGQDQGSAYAnR 2014(16); 2014(32); 2014(32);
91 QLVHSFAEGQDQGSAYAnR 2007(62); 2009(62); 2009(62); 2009(62); 2009(62); 2009(57); 2011(50); 2012(50); 2012(52); 2012(34); 2013(34); 2013(34); 2013(40); 2013(45); 2013(14); 2014(15); 2014(18); 2014(33); 2014(1); 2015(2); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015;2015;2007;2007;2007;2007;2007;
91 DQGSAYAnRTALFPDLLAQGNASLR 2011(57); 2011(58); 2012(52); 2015(12);
91 QLVHSFAEGQDQGSAYAnRTALFPDLLAQGNASLR 2011(57);
91 QLVHSFAEGQDQGSAYAnRTALFPDLLAQGNASLRLQR 2013(38);
104 LAQGnASLRL 2009(64);
104 LLAQGnASLR 2014(33);
104 PDLLAQGnASLR 2007(unpublished);
104 DIIAQGnASIRIQ 2013(37);
104 TAIFPDIIAQGnASIR 2014(16); 2014(32); 2014(32);
104 TALFPDLLAQGnASLR 2003( 83); 2007(72); 2009(62); 2009(62); 2009(62); 2009(62); 2009(62); 2009(63); 2009(67); 2011(57); 2011(58); 2012(50); 2012(50); 2012(51); 2012(52); 2013(34); 2013(34); 2013(34); 2013(45); 2014(14); 2014(15); 2014(17); 2014(18); 2014(22); 2014(33); 2015(1); 2015(2); 2015(9); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2007(Unpublished ); 2007;2007;2007;2007;2007;2007;2007 ;
104 ANRTALFPDLLAQGnASL 2014(33);
104 DQGSAYANRTALFPDLLAQGnASLR 2011(57); 2011(58); 2012(52); 2015(12);
104 QLVHSFAEGQDQGSAYANRTALFPDLLAQGnASLR 2011(57);
104 QLVHSFAEGQDQGSAYANRTALFPDLLAQGnASLRLQR 2013(38);
189 TGnVTTSQMANEQGLF 2014(33);
189 VFWQDGQGVPLTGnVT 2014(33);
189 nVTTSQMANEQGLFDVHSILR 2015(1);
215 nGTYSCLVR 2015(1);
215 VVLGAnGTY 2014(33);
215 RVVLGAnGTY 2014(33);
215 LGAnGTYSCLVR 2012(48);
215 RVVIGAnGTYSCI 2013(37);
215 VVIGAnGTYSCIVR 2014(16);
215 VVLGAnGTYSCLVR 2007(72); 2007(75); 2007(62); 2009(62); 2009(62); 2009(62); 2009(67); 2009(48); 2012(50); 2012(50); 2012(52); 2012(34); 2013(34); 2013(40); 2013(42); 2013(45); 2013(14); 2014(15); 2014(18); 2014(33); 2014(1); 2015(2); 2015(9); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(Unpublished ); 2015;2007;2007;2007;2007;2007;2007 ;
309 AnRTALF 2014(33);
309 DQGSAYAnR 2005( 81); 2012(50); 2013(34); 2013(34); 2013(34); 2013(40); 2013(45); 2014(14); 2014(15); 2014(22); 2014(33); 2015(2); 2015(10); 2015(unpublished);2015(unpublished);2015;2015;2015;2015;2007;2007;
309 AnRTALFPDLL 2014(33);
309 QGSAYAnRTAIFP 2013(37);
309 AnRTALFPDLLAQGNASL 2014(33);
309 DQGSAYAnRTALFPDLLAQGNASLR 2011(57); 2011(58); 2012(52); 2015(12);
322 LAQGnASLRL 2009(64);
322 LLAQGnASLR 2014(33);
322 PDLLAQGnASLR 2007(unpublished);
322 DIIAQGnASIRIQ 2013(37);
322 TAIFPDIIAQGnASIR 2014(16); 2014(32); 2014(32);
322 TALFPDLLAQGnASLR 2003( 83); 2007(72); 2009(62); 2009(62); 2009(62); 2009(62); 2009(62); 2009(63); 2009(67); 2011(57); 2011(58); 2012(50); 2012(50); 2012(51); 2012(52); 2013(34); 2013(34); 2013(34); 2013(45); 2014(14); 2014(15); 2014(17); 2014(18); 2014(22); 2014(33); 2015(1); 2015(2); 2015(9); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2007(Unpublished ); 2007;2007;2007;2007;2007;2007;2007 ;
322 ANRTALFPDLLAQGnASL 2014(33);
322 DQGSAYANRTALFPDLLAQGnASLR 2011(57); 2011(58); 2012(52); 2015(12);
407 TGnVTTSQMANEQGLF 2014(33);
407 VFWQDGQGVPLTGnVT 2014(33);
407 nVTTSQMANEQGLFDVHSVLR 2015(1);
407 GYPEAEVFWQDGQGVPLTGnVTTSQMANEQGLFDVHSVLR 2009(62); 2009(62); 2009(64); 2012(52); 2015(unpublished);2015(1); 2015(2);
433 nGTYSCLVR 2015(1);
433 VVLGAnGTY 2014(33);
433 RVVLGAnGTY 2014(33);
433 LGAnGTYSCLVR 2012(48);
433 RVVIGAnGTYSCI 2013(37);
433 VVIGAnGTYSCIVR 2014(16);
433 VVLGAnGTYSCLVR 2007(72); 2007(75); 2007(62); 2009(62); 2009(62); 2009(62); 2009(67); 2009(48); 2012(50); 2012(50); 2012(52); 2012(34); 2013(34); 2013(40); 2013(42); 2013(45); 2013(14); 2014(15); 2014(18); 2014(33); 2014(1); 2015(2); 2015(9); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(Unpublished ); 2015;2007;2007;2007;2007;2007;2007 ;

Sequence

1112131415161718191
MLRRRGSPGMGVHVGAALGALWFCLTGALEVQVPEDPVVALVGTDATLCCSFSPEPGFSLAQLNLIWQLTDTKQLVHSFAEGQDQGSAYANRTALFPDLL
101111121131141151161171181191
AQGNASLRLQRVRVADEGSFTCFVSIRDFGSAAVSLQVAAPYSKPSMTLEPNKDLRPGDTVTITCSSYQGYPEAEVFWQDGQGVPLTGNVTTSQMANEQG
201211221231241251261271281291
LFDVHSILRVVLGANGTYSCLVRNPVLQQDAHSSVTITPQRSPTGAVEVQVPEDPVVALVGTDATLRCSFSPEPGFSLAQLNLIWQLTDTKQLVHSFTEG
301311321331341351361371381391
RDQGSAYANRTALFPDLLAQGNASLRLQRVRVADEGSFTCFVSIRDFGSAAVSLQVAAPYSKPSMTLEPNKDLRPGDTVTITCSSYRGYPEAEVFWQDGQ
401411421431441451461471481491
GVPLTGNVTTSQMANEQGLFDVHSVLRVVLGANGTYSCLVRNPVLQQDAHGSVTITGQPMTFPPEALWVTVGLSVCLIALLVALAFVCWRKIKQSCEEEN
501511521531
AGAEDQDGEGEGSKTALQPLKHSDSKEDDGQEIA

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
9Smeekens JM, Chen WX, Wu RH: Mass Spectrometric Analysis of the Cell Surface N-Glycoproteome by Combining Metabolic Labeling and Click Chemistry. Journal of the American Society for Mass Spectrometry 2015, 26:604-614.
10Tan ZJ, Yin HD, Nie S, Lin ZX, Zhu JH, Ruffin MT, Anderson MA, Simone DM, Lubman DM: Large-Scale Identification of Core-Fucosylated Glycopeptide Sites in Pancreatic Cancer Serum Using Mass Spectrometry. Journal of Proteome Research 2015, 14:1968-1978.
12Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
15Chen R, Seebun D, Ye M, Zou H, Figeys D: Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS. Journal of Proteomics 2014, 103:194-203.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
17Fang C, Xiong Z, Qin H, Huang G, Liu J, Ye M, Feng S, Zou H: One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides. Analytica Chimica Acta 2014, 841:99-105.
18Hirao Y, Matsuzaki H, Iwaki J, Kuno A, Kaji H, Ohkura T, Togayachi A, Abe M, Nomura M, Noguchi M, et al: Glycoproteomics Approach for Identifying Glycobiomarker Candidate Molecules for Tissue Type Classification of Non-small Cell Lung Carcinoma. Journal of Proteome Research 2014, 13:4705-4716.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
38Kaji H, Ocho M, Togayachi A, Kuno A, Sogabe M, Ohkura T, Nozaki H, Angata T, Chiba Y, Ozaki H, et al: Glycoproteomic Discovery of Serological Biomarker Candidates for HCV/HBV Infection-Associated Liver Fibrosis and Hepatocellular Carcinoma. Journal of Proteome Research 2013, 12:2630-2640.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
42Ma C, Zhao X, Han H, Tong W, Zhang Q, Qin P, Chang C, Peng B, Ying W, Qian X: N-linked glycoproteome profiling of human serum using tandem enrichment and multiple fraction concatenation. Electrophoresis 2013, 34:2440-2450.
45Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
50Whitmore TE, Peterson A, Holzman T, Eastham A, Amon L, McIntosh M, Ozinsky A, Nelson PS, Martin DB: Integrative Analysis of N-Linked Human Glycoproteomic Data Sets Reveals PTPRF Ectodomain as a Novel Plasma Biomarker Candidate for Prostate Cancer. Journal of Proteome Research 2012, 11:2653-2665.
51Yeh C-H, Chen S-H, Li D-T, Lin H-P, Huang H-J, Chang C-I, Shih W-L, Chern C-L, Shi F-K, Hsu J-L: Magnetic bead-based hydrophilic interaction liquid chromatography for glycopeptide enrichments. Journal of Chromatography A 2012, 1224:70-78.
52Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667.
57Chen Y, Cao J, Yan G, Lu H, Yang P: Two-step protease digestion and glycopeptide capture approach for accurate glycosite identification and glycoprotein sequence coverage improvement. Talanta 2011, 85:70-75.
58Nagano K, Shinkawa T, Kato K, Inomata N, Yabuki N, Haramura M: Distinct cell surface proteome profiling by biotin labeling and glycoprotein capturing. Journal of Proteomics 2011, 74:1985-1993.
62Arcinas A, Yen T-Y, Kebebew E, Macher BA: Cell Surface and Secreted Protein Profiles of Human Thyroid Cancer Cell Lines Reveal Distinct Glycoprotein Patterns. Journal of Proteome Research 2009, 8:3958-3968.
63Cao J, Shen C, Wang H, Shen H, Chen Y, Nie A, Yan G, Lu H, Liu Y, Yang P: Identification of N-Glycosylation Sites on Secreted Proteins of Human Hepatocellular Carcinoma Cells with a Complementary Proteomics Approach. Journal of Proteome Research 2009, 8:662-672.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
67McDonald CA, Yang JY, Marathe V, Yen T-Y, Macher BA: Combining Results from Lectin Affinity Chromatography and Glycocapture Approaches Substantially Improves the Coverage of the Glycoproteome. Molecular & Cellular Proteomics 2009, 8:287-301.
72Hanson SR, Hsu T-L, Weerapana E, Kishikawa K, Simon GM, Cravatt BF, Wong C-H: Tailored glycoproteomics and glycan site mapping using saccharide-selective bioorthogonal probes. Journal of the American Chemical Society 2007, 129:7266-+.
75Sun B, Ranish JA, Utleg AG, White JT, Yan X, Lin B, Hood L: Shotgun glycopeptide capture approach coupled with mass Spectrometry for comprehensive glycoproteomics. Molecular & Cellular Proteomics 2007, 6:141-149.