Contains      

UniProtKB-Q6P179

Protein Endoplasmic reticulum aminopeptidase 2
Gene ERAP2
Status Reviewed
Source Breast Cancer cell lines; Colorectal tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); HCC cell lines (Liver); HCC cell lines (Liver)Jurkat T cell line; Jurkat T cell line; LNCap/PC3 cell lines (Prostate cancer); Liver; Liver tumor (HCC); OVCAR-3 cell line (Ovarian cancer); Ovarian tumor; Pancreatic islets; Plasma; Platelet; Prostate; Prostate tumor; SKOV-3 cell line (Ovarian cancer); SW1990 cell line (Pancreatic cancer); Serum; Urine; UrineJurkat T cell line
Years 2005-2015

Glycosites

Site Identified Peptides Year(Publication ID)
85 VHPnLTSL 2014(33);
85 VHPnLTSLDF 2014(33);
85 DLFVHPnLTSL 2014(33);
85 DLFVHPnLTSLD 2014(33);
85 DLFVHPnLTSLDF 2014(33);
85 VHPnLTSLDFVASEK 2014(33);
85 DLFVHPnLTSLDFVASE 2014(33);
85 LFVHPnLTSLDFVASEK 2014(33);
85 DLFVHPnLTSLDFVASEK 2014(33);
85 SVVIPLHYDLFVHPnLTSLDFVASEK 2015(unpublished);
85 IPSVVIPIHYDIFVHPnITSIDFVASEK 2014(16);
85 LPSVVIPLHYDLFVHPnLTSLDFVASEK 2014(33); 2015(unpublished);
103 VLVSnATQF 2014(33);
103 IEVLVSnATQF 2014(33);
103 IEVLVSnATQFIILH 2015(unpublished);
103 VLVSnATQFIILHSK 2014(33);
103 IEVIVSnATQFIIIHSK 2014(16); 2014(32); 2014(32);
103 IEVLVSnATQFIILHSK 2013(34); 2014(13); 2014(33); 2015(unpublished);2015(unpublished);2015(2); 2015(10);
119 ITnATLQSE 2014(33);
119 ITnATLQSEE 2014(33);
119 ITnATLQSEEDSR 2014(33);
119 KDIEITnATIQSE 2013(37);
119 DIEITnATIQSEEDSR 2014(16);
119 DLEITnATLQSEEDSR 2005( 81); 2012(47); 2012(48); 2013(34); 2013(34); 2013(40); 2014(13); 2014(14); 2014(22); 2014(33); 2015(1); 2015(2); 2015(unpublished);2015(unpublished);2015(unpublished);2015;2015;2007;2007;2007;
119 HSKDLEITnATLQSEEDSRY 2014(33);
119 IILHSKDLEITnATLQSEEDSRY 2014(33);
219 AnFSIK 2014(33); 2015(2);
219 AnFSIKIRR 2007(unpublished);2007(unpublished);2007;
294 ASPDKRnQTHY 2014(33);
294 nQTHYALQASLK 2013(40); 2014(13); 2014(33); 2015(unpublished);
294 RnQTHYAIQASIK 2014(16);
405 LIAVnATYPE 2014(33);
431 DSLnSSRPISK 2012(48); 2014(14); 2014(33); 2015(unpublished);2015(unpublished);
431 ITKDSInSSRPIS 2013(37);
431 EVITKDSLnSSRPI 2014(33);
431 DSLnSSRPISKPAETPTQIQE 2014(33);
431 VITKDSLnSSRPISKPAETPTQIQE 2014(33);
431 DSInSSRPISKPAETPTQIQEMFDEVSYNK 2014(16);
650 NQnHTLL 2014(33);
650 EGHGWDQLITQLNQnHTLL 2014(33);
650 GHGWDQLITQLNQnHTLLRPK 2014(33);
650 FNVDSNGYYIVHYEGHGWDQLITQLNQnHTLLRPK 2015(unpublished);
714 nISDISENLK 2013(40); 2014(33); 2015(unpublished);
714 nISDISENLKR 2014(33); 2015(unpublished);2015(unpublished);
714 RnISDISENLK 2012(48); 2014(33); 2015(unpublished);
714 RnISDISENIKR 2014(16);
714 RnISDISENLKR 2007(13); 2014(unpublished);2007(unpublished);2007;

Sequence

1112131415161718191
MFHSSAMVNSHRKPMFNIHRGFYCLTAILPQICICSQFSVPSSYHFTEDPGAFPVATNGERFPWQELRLPSVVIPLHYDLFVHPNLTSLDFVASEKIEVL
101111121131141151161171181191
VSNATQFIILHSKDLEITNATLQSEEDSRYMKPGKELKVLSYPAHEQIALLVPEKLTPHLKYYVAMDFQAKLGDGFEGFYKSTYRTLGGETRILAVTDFE
201211221231241251261271281291
PTQARMAFPCFDEPLFKANFSIKIRRESRHIALSNMPKVKTIELEGGLLEDHFETTVKMSTYLVAYIVCDFHSLSGFTSSGVKVSIYASPDKRNQTHYAL
301311321331341351361371381391
QASLKLLDFYEKYFDIYYPLSKLDLIAIPDFAPGAMENWGLITYRETSLLFDPKTSSASDKLWVTRVIAHELAHQWFGNLVTMEWWNDIWLKEGFAKYME
401411421431441451461471481491
LIAVNATYPELQFDDYFLNVCFEVITKDSLNSSRPISKPAETPTQIQEMFDEVSYNKGACILNMLKDFLGEEKFQKGIIQYLKKFSYRNAKNDDLWSSLS
501511521531541551561571581591
NSCLESDFTSGGVCHSDPKMTSNMLAFLGENAEVKEMMTTWTLQKGIPLLVVKQDGCSLRLQQERFLQGVFQEDPEWRALQERYLWHIPLTYSTSSSNVI
601611621631641651661671681691
HRHILKSKTDTLDLPEKTSWVKFNVDSNGYYIVHYEGHGWDQLITQLNQNHTLLRPKDRVGLIHDVFQLVGAGRLTLDKALDMTYYLQHETSSPALLEGL
701711721731741751761771781791
SYLESFYHMMDRRNISDISENLKRYLLQYFKPVIDRQSWSDKGSVWDRMLRSALLKLACDLNHAPCIQKAAELFSQWMESSGKLNIPTDVLKIVYSVGAQ
801811821831841851861871881891
TTAGWNYLLEQYELSMSSAEQNKILYALSTSKHQEKLLKLIELGMEGKVIKTQNLAALLHAIARRPKGQQLAWDFVRENWTHLLKKFDLGSYDIRMIISG
901911921931941951
TTAHFSSKDKLQEVKLFFESLEAQGSHLDIFQTVLETITKNIKWLEKNLPTLRTWLMVNT

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
10Tan ZJ, Yin HD, Nie S, Lin ZX, Zhu JH, Ruffin MT, Anderson MA, Simone DM, Lubman DM: Large-Scale Identification of Core-Fucosylated Glycopeptide Sites in Pancreatic Cancer Serum Using Mass Spectrometry. Journal of Proteome Research 2015, 14:1968-1978.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
47Almaraz RT, Tian Y, Bhattarcharya R, Tan E, Chen S-H, Dallas MR, Chen L, Zhang Z, Zhang H, Konstantopoulos K: Metabolic flux increases glycoprotein sialylation: implications for cell adhesion and cancer metastasis. Molecular & Cellular Proteomics 2012, 11:M112. 017558.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.