Detail

UniProtKB-Q6ZMP0


Protein	Thrombospondin type-1 domain-containing protein 4
Gene	THSD4
Status	Reviewed
Source	Breast Cancer cell lines; Breast cancer xenografts; Colorectal tumor; Liver; Non-small Cell Lung Carcinoma; OVCAR-3 cell line (Ovarian cancer); Ovarian tumor; Prostate; Urine
Years	2013-2015

Glycosites

Site	Identified Peptides	Year(Publication ID)
373	nGTAICVSGQCK	2015(1);
373	GTPCDQnGTAICV	2013(37);
373	VIDGTPCDQnGTAICVSGQCK	2014(33); 2015(unpublished);2015(unpublished);
373	QAEKVIDGTPCDQnGTAICVSGQCK	2014(18);
441	InITEMYK	2014(14); 2014(22); 2014(33); 2015(unpublished);2015(1); 2015(unpublished);2015(unpublished);2015(unpublished);
441	GATKInITE	2014(33);
441	HRVVEIPEGATKInITEMY	2014(33);
709	QVYAnR	2015(unpublished);

Sequence

1	11	21	31	41	51	61	71	81	91
MVSHFMGSLS	VLCFLLLLGF	QFVCPQPSTQ	HRKVPQRMAA	EGAPEDDGGG	GAPGVWGAWG	PWSACSRSCS	GGVMEQTRPC	LPRSYRLRGG	QRPGAPARAF
101	111	121	131	141	151	161	171	181	191
ADHVVSAVRT	SVPLHRSRDE	TPALAGTDAS	RQGPTVLRGS	RHPQPQGLEV	TGDRRSRTRG	TIGPGKYGYG	KAPYILPLQT	DTAHTPQRLR	RQKLSSRHSR
201	211	221	231	241	251	261	271	281	291
SQGASSARHG	YSSPAHQVPQ	HGPLYQSDSG	PRSGLQAAEA	PIYQLPLTHD	QGYPAASSLF	HSPETSNNHG	VGTHGATQSF	SQPARSTAIS	CIGAYRQYKL
301	311	321	331	341	351	361	371	381	391
CNTNVCPESS	RSIREVQCAS	YNNKPFMGRF	YEWEPFAEVK	GNRKCELNCQ	AMGYRFYVRQ	AEKVIDGTPC	DQNGTAICVS	GQCKSIGCDD	YLGSDKVVDK
401	411	421	431	441	451	461	471	481	491
CGVCGGDNTG	CQVVSGVFKH	ALTSLGYHRV	VEIPEGATKI	NITEMYKSNN	YLALRSRSGR	SIINGNWAID	RPGKYEGGGT	MFTYKRPNEI	SSTAGESFLA
501	511	521	531	541	551	561	571	581	591
EGPTNEILDV	YMIHQQPNPG	VHYEYVIMGT	NAISPQVPPH	RRPGEPFNGQ	MVTEGRSQEE	GEQKGRNEEK	EDLRGEAPEM	FTSESAQTFP	VRHPDRFSPH
601	611	621	631	641	651	661	671	681	691
RPDNLVPPAP	QPPRRSRDHN	WKQLGTTECS	TTCGKGSQYP	IFRCVHRSTH	EEAPESYCDS	SMKPTPEEEP	CNIFPCPAFW	DIGEWSECSK	TCGLGMQHRQ
701	711	721	731	741	751	761	771	781	791
VLCRQVYANR	SLTVQPYRCQ	HLEKPETTST	CQLKICSEWQ	IRTDWTSCSV	PCGVGQRTRD	VKCVSNIGDV	VDDEECNMKL	RPNDIENCDM	GPCAKSWFLT
801	811	821	831	841	851	861	871	881	891
EWSERCSAEC	GAGVRTRSVV	CMTNHVSSLP	LEGCGNNRPA	EATPCDNGPC	TGKVEWFAGS	WSQCSIECGS	GTQQREVICV	RKNADTFEVL	DPSECSFLEK
901	911	921	931	941	951	961	971	981	991
PPSQQSCHLK	PCGAKWFSTE	WSMCSKSCQG	GFRVREVRCL	SDDMTLSNLC	DPQLKPEERE	SCNPQDCVPE	VDENCKDKYY	NCNVVVQARL	CVYNYYKTAC
1001	1011
CASCTRVANR	QTGFLGSR

Reference

ID	Publication
1	Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
14	Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
18	Hirao Y, Matsuzaki H, Iwaki J, Kuno A, Kaji H, Ohkura T, Togayachi A, Abe M, Nomura M, Noguchi M, et al: Glycoproteomics Approach for Identifying Glycobiomarker Candidate Molecules for Tissue Type Classification of Non-small Cell Lung Carcinoma. Journal of Proteome Research 2014, 13:4705-4716.
22	Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
33	Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
37	Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.