Detail

UniProtKB-Q7Z5N4


Protein	Protein sidekick-1
Gene	SDK1
Status	Reviewed
Source	Breast cancer xenografts; Colorectal tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); Liver; OVCAR-3 cell line (Ovarian cancer); Ovarian tumor; Pancreatic islets; Serum; Urine
Years	2012-2015

Glycosites

Site	Identified Peptides	Year(Publication ID)
271	NGEnKTSPFIHLSIAR	2013(45);
301	DVGTPETMAPTIVVPPGnR	2013(45); 2015(unpublished);2015(unpublished);
1024	ITHTInSTTHEYK	2014(16);
1024	LTHTLnSTTHEYK	2012(48); 2014(33); 2015(unpublished);2015(10);
1282	ESVPSAAPEnVSAEAVSS	2012(48);
1333	GnHTQSALLAGLR	2015(unpublished);2015(unpublished);
1748	IYYWEADSQnETEK	2012(48); 2014(22);
1748	IYYWEADSQnETEKMK	2014(16);
1767	LKnLTSHTK	2015(unpublished);
1893	nITAGPAEGSPGSPR	2015(1);
1893	TITYGPELQAnITAGPAEGSPGSPR	2015(unpublished);

Sequence

1	11	21	31	41	51	61	71	81	91
MARGARPSAA	GGGGGGAEPP	ERAGPGRPRG	SPPGRARPSL	APRPGPEPSR	PRAAPETSGG	DTAGAGRCGG	RRAAKLGPGR	RGWWALLALQ	LHLLRALAQD
101	111	121	131	141	151	161	171	181	191
DVAPYFKTEP	GLPQIHLEGN	RLVLTCLAEG	SWPLEFKWMR	DDSELTTYSS	EYKYIIPSLQ	KLDAGFYRCV	VRNRMGALLQ	RKSEVQVAYM	GSFMDTDQRK
201	211	221	231	241	251	261	271	281	291
TVSQGRAAIL	NLLPITSYPR	PQVTWFREGH	KIIPSNRIAI	TLENQLVILA	TTTSDAGAYY	VQAVNEKNGE	NKTSPFIHLS	IARDVGTPET	MAPTIVVPPG
301	311	321	331	341	351	361	371	381	391
NRSVVAGSSE	TTLECIASAR	PVEDLSVTWK	RNGVRITSGL	HSFGRRLTIS	NPTSADTGPY	VCEAALPGSA	FEPARATAFL	FIIEPPYFTA	EPESRISAEV
401	411	421	431	441	451	461	471	481	491
EETVDIGCQA	MGVPLPTLQW	YKDAISISRL	QNPRYKVLAS	GGLRIQKLRP	EDSGIFQCFA	SNEGGEIQTH	TYLDVTNIAP	VFTQRPVDTT	VTDGMTAILR
501	511	521	531	541	551	561	571	581	591
CEVSGAPKPA	ITWKRENHIL	ASGSVRIPRF	MLLESGGLQI	APVFIQDAGN	YTCYAANTEG	SLNASATLTV	WNRTSIVHPP	EDHVVIKGTT	ATLHCGATHD
601	611	621	631	641	651	661	671	681	691
PRVSLRYVWK	KDNVALTPSS	TSRIVVEKDG	SLLISQTWSG	DIGDYSCEIV	SEGGNDSRMA	RLEVIELPHS	PQNLLVSPNS	SHSHAVVLSW	VRPFDGNSPI
701	711	721	731	741	751	761	771	781	791
LYYIVELSEN	NSPWKVHLSN	VGPEMTGVTV	SGLTPARTYQ	FRVCAVNEVG	RGQYSAETSR	LMLPEEPPSA	PPKNIVASGR	TNQSIMVQWQ	PPPETEHNGV
801	811	821	831	841	851	861	871	881	891
LRGYILRYRL	AGLPGEYQQR	NITSPEVNYC	LVTDLIIWTQ	YEIQVAAYNG	AGLGVFSRAV	TEYTLQGVPT	APPQNVQTEA	VNSTTIQFLW	NPPPQQFING
901	911	921	931	941	951	961	971	981	991
INQGYKLLAW	PADAPEAVTV	VTIAPDFHGV	HHGHITNLKK	FTAYFTSVLC	FTTPGDGPPS	TPQLVWTQED	KPGAVGHLSF	TEILDTSLKV	SWQEPLEKNG
1001	1011	1021	1031	1041	1051	1061	1071	1081	1091
IITGYQISWE	VYGRNDSRLT	HTLNSTTHEY	KIQGLSSLTT	YTIDVAAVTA	VGTGLVTSST	ISSGVPPDLP	GAPSNLVISN	ISPRSATLQF	RPGYDGKTSI
1101	1111	1121	1131	1141	1151	1161	1171	1181	1191
SRWIVEGQVG	AIGDEEEWVT	LYEEENEPDA	QMLEIPNLTP	YTHYRFRMKQ	VNIVGPSPYS	PSSRVIQTLQ	APPDVAPTSV	TVRTASETSL	RLRWVPLPDS
1201	1211	1221	1231	1241	1251	1261	1271	1281	1291
QYNGNPESVG	YRIKYWRSDL	QSSAVAQVVS	DRLEREFTIE	ELEEWMEYEL	QMQAFNAVGA	GPWSEVVRGR	TRESVPSAAP	ENVSAEAVSS	TQILLTWTSV
1301	1311	1321	1331	1341	1351	1361	1371	1381	1391
PEQDQNGLIL	GYKILFRAKD	LDPEPRSHIV	RGNHTQSALL	AGLRKFVLYE	LQVLAFTRIG	NGVPSTPLIL	ERTKDDAPGP	PVRLVFPEVR	LTSVRIVWQP
1401	1411	1421	1431	1441	1451	1461	1471	1481	1491
PEEPNGIILG	YQIAYRLASS	SPHTFTTVEV	GATVRQFTAT	DLAPESAYIF	RLSAKTRQGW	GEPLEATVIT	TEKRERPAPP	RELLVPQAEV	TARSLRLQWV
1501	1511	1521	1531	1541	1551	1561	1571	1581	1591
PGSDGASPIR	YFTMQVRELP	RGEWQTYSSS	ISHEATACVV	DRLRPFTSYK	LRLKATNDIG	DSDFSSETEA	VTTLQDVPGE	PPGSVSATPH	TTSSVLIQWQ
1601	1611	1621	1631	1641	1651	1661	1671	1681	1691
PPRDESLNGL	LQGYRIYYRE	LEYEAGSGTE	AKTLKNPIAL	HAELTAQSSF	KTVNSSSTST	MCELTHLKKY	RRYEVIMTAY	NIIGESPASA	PVEVFVGEAA
1701	1711	1721	1731	1741	1751	1761	1771	1781	1791
PAMAPQNVQV	TPLTASQLEV	TWDPPPPESQ	NGNIQGYKIY	YWEADSQNET	EKMKVLFLPE	PVVRLKNLTS	HTKYLVSISA	FNAAGDGPKS	DPQQGRTHQA
1801	1811	1821	1831	1841	1851	1861	1871	1881	1891
APGAPSFLAF	SEITSTTLNV	SWGEPAAANG	ILQGYRVVYE	PLAPVQGVSK	VVTVEVRGNW	QRWLKVRDLT	KGVTYFFRVQ	ARTITYGPEL	QANITAGPAE
1901	1911	1921	1931	1941	1951	1961	1971	1981	1991
GSPGSPRDVL	VTKSASELTL	QWTEGHSGDT	PTTGYVIEAR	PSDEGLWDMF	VKDIPRSATS	YTLSLDKLRQ	GVTYEFRVVA	VNEAGYGEPS	NPSTAVSAQV
2001	2011	2021	2031	2041	2051	2061	2071	2081	2091
EAPFYEEWWF	LLVMALSSLI	VILLVVFALV	LHGQNKKYKN	CSTGKGISTM	EESVTLDNGG	FAALELSSRH	LNVKSTFSKK	NGTRSPPRPS	PGGLHYSDED
2101	2111	2121	2131	2141	2151	2161	2171	2181	2191
ICNKYNGAVL	TESVSLKEKS	ADASESEATD	SDYEDALPKH	SFVNHYMSDP	TYYNSWKRRA	QGRAPAPHRY	EAVAGSEAGA	QLHPVITTQS	AGGVYTPAGP
2201	2211
GARTPLTGFS	SFV

Reference

ID	Publication
1	Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
10	Tan ZJ, Yin HD, Nie S, Lin ZX, Zhu JH, Ruffin MT, Anderson MA, Simone DM, Lubman DM: Large-Scale Identification of Core-Fucosylated Glycopeptide Sites in Pancreatic Cancer Serum Using Mass Spectrometry. Journal of Proteome Research 2015, 14:1968-1978.
16	Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
22	Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
33	Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
45	Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991.
48	Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.