Detail

UniProtKB-Q8IWK6


Protein	Adhesion G protein-coupled receptor A3
Gene	ADGRA3
Status	Reviewed
Source	Breast Cancer cell lines; Breast cancer xenografts; LNCap/PC3 cell lines (Prostate cancer); Liver; Lymphocytes; OVCAR-3 cell line (Ovarian cancer); Ovarian tumor; Prostate cancer metastasis to liver; Prostate tumor; Serum
Years	2007-2015

Glycosites

Site	Identified Peptides	Year(Publication ID)
98	nGSFSGLSLLER	2015(1); 2015(unpublished);
98	ISELKnGSFSGLSLLER	2015(1);
206	nITVR	2014(33);
206	nLTVR	2014(33);
206	EKnITVR	2015(2);
433	nLTNAVATAR	2015(1);
433	VLYMFNQMPLnLTNAVATAR	2015(1); 2007(unpublished);2007(unpublished);2007 (Unpublished );
592	QISFKCnVSNTFS	2013(37);
652	nSTNLADDGK	2015(1);
652	nSTNLADDGKR	2015(1);
652	LFPATGnSTNLADDGK	2015(1); 2015(unpublished);2007(unpublished);2007;
652	LFPATGnSTNLADDGKR	2015(2);
687	IDGVNVDTHHIPVnVTLR	2014(33); 2015(unpublished);2015(1); 2015(2);

Sequence

1	11	21	31	41	51	61	71	81	91
MEPPGRRRGR	AQPPLLLPLS	LLALLALLGG	GGGGGAAALP	AGCKHDGRPR	GAGRAAGAAE	GKVVCSSLEL	AQVLPPDTLP	NRTVTLILSN	NKISELKNGS
101	111	121	131	141	151	161	171	181	191
FSGLSLLERL	DLRNNLISSI	DPGAFWGLSS	LKRLDLTNNR	IGCLNADIFR	GLTNLVRLNL	SGNLFSSLSQ	GTFDYLASLR	SLEFQTEYLL	CDCNILWMHR
201	211	221	231	241	251	261	271	281	291
WVKEKNITVR	DTRCVYPKSL	QAQPVTGVKQ	ELLTCDPPLE	LPSFYMTPSH	RQVVFEGDSL	PFQCMASYID	QDMQVLWYQD	GRIVETDESQ	GIFVEKNMIH
301	311	321	331	341	351	361	371	381	391
NCSLIASALT	ISNIQAGSTG	NWGCHVQTKR	GNNTRTVDIV	VLESSAQYCP	PERVVNNKGD	FRWPRTLAGI	TAYLQCTRNT	HGSGIYPGNP	QDERKAWRRC
401	411	421	431	441	451	461	471	481	491
DRGGFWADDD	YSRCQYANDV	TRVLYMFNQM	PLNLTNAVAT	ARQLLAYTVE	AANFSDKMDV	IFVAEMIEKF	GRFTKEEKSK	ELGDVMVDIA	SNIMLADERV
501	511	521	531	541	551	561	571	581	591
LWLAQREAKA	CSRIVQCLQR	IATYRLAGGA	HVYSTYSPNI	ALEAYVIKST	GFTGMTCTVF	QKVAASDRTG	LSDYGRRDPE	GNLDKQLSFK	CNVSNTFSSL
601	611	621	631	641	651	661	671	681	691
ALKNTIVEAS	IQLPPSLFSP	KQKRELRPTD	DSLYKLQLIA	FRNGKLFPAT	GNSTNLADDG	KRRTVVTPVI	LTKIDGVNVD	THHIPVNVTL	RRIAHGADAV
701	711	721	731	741	751	761	771	781	791
AARWDFDLLN	GQGGWKSDGC	HILYSDENIT	TIQCYSLSNY	AVLMDLTGSE	LYTQAASLLH	PVVYTTAIIL	LLCLLAVIVS	YIYHHSLIRI	SLKSWHMLVN
801	811	821	831	841	851	861	871	881	891
LCFHIFLTCV	VFVGGITQTR	NASICQAVGI	ILHYSTLATV	LWVGVTARNI	YKQVTKKAKR	CQDPDEPPPP	PRPMLRFYLI	GGGIPIIVCG	ITAAANIKNY
901	911	921	931	941	951	961	971	981	991
GSRPNAPYCW	MAWEPSLGAF	YGPASFITFV	NCMYFLSIFI	QLKRHPERKY	ELKEPTEEQQ	RLAANENGEI	NHQDSMSLSL	ISTSALENEH	TFHSQLLGAS
1001	1011	1021	1031	1041	1051	1061	1071	1081	1091
LTLLLYVALW	MFGALAVSLY	YPLDLVFSFV	FGATSLSFSA	FFVVHHCVNR	EDVRLAWIMT	CCPGRSSYSV	QVNVQPPNSN	GTNGEAPKCP	NSSAESSCTN
1101	1111	1121	1131	1141	1151	1161	1171	1181	1191
KSASSFKNSS	QGCKLTNLQA	AAAQCHANSL	PLNSTPQLDN	SLTEHSMDND	IKMHVAPLEV	QFRTNVHSSR	HHKNRSKGHR	ASRLTVLREY	AYDVPTSVEG
1201	1211	1221	1231	1241	1251	1261	1271	1281	1291
SVQNGLPKSR	LGNNEGHSRS	RRAYLAYRER	QYNPPQQDSS	DACSTLPKSS	RNFEKPVSTT	SKKDALRKPA	VVELENQQKS	YGLNLAIQNG	PIKSNGQEGP
1301	1311	1321
LLGTDSTGNV	RTGLWKHETT	V

Reference

ID	Publication
1	Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2	Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
33	Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
37	Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.