Contains      

UniProtKB-Q8TCJ2

Protein Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3B
Gene STT3B
Status Reviewed
Source 22Rv1 cell line (prostate cancer); Breast Cancer cell lines; Breast cancer xenografts; Breast cancer xenograftsPBMC Macrophage cells; Breast tumor; Colorectal cancer cell line, HCT-116 (colorectal cancer); Colorectal tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); HCC cell lines (Liver)Jurkat T cell line; HEK293 cell line (embryonic kidney); HEK293T cell line (embryonic kidney), HEK293T cell line (embryonic kidney); Hela cell line (cervical cancer); IGROV-1/CP cell line (Ovarian cancer)IGROV-1/CP; K562A cell line (Leukemia); LNCap/PC3 cell lines (Prostate cancer); Liver; Liver tumor (HCC); Non-small Cell Lung Carcinoma; OVCAR-3 cell line (Ovarian cancer); Ovarian tumor; Ovarian tumorJurkat T cell line; Pancreatic islets; Platelet; Prostate tumor; SKOV-3 cell line (Ovarian cancer); Serum; Spermatozoa; ovarian tumor
Years 2007-2015

Glycosites

Site Identified Peptides Year(Publication ID)
616 GYQIAGMAnR 2014(33);
616 IAGMAnRTTL 2014(33);
616 QIAGMAnRTTL 2014(33);
616 AnRTTLVDNNTW 2014(33);
616 DYGYQIAGMAnR 2014(33);
616 GYQIAGMAnRTTL 2014(33);
616 MAnRTTLVDNNTW 2014(33);
616 DYGYQIAGMAnRTTL 2014(33);
616 IAGMAnRTTLVDNNTW 2014(33);
616 QIAGMAnRTTLVDNNTW 2014(33);
616 VMSWWDYGYQIAGMAnR 2012(48); 2013(43); 2014(15); 2014(16); 2014(19); 2014(33); 2015(unpublished);2015(1); 2015(2);
616 nRTTLVDNNTWNNSHIALVGK 2014(33);
616 QIAGMAnRTTLVDNNTWNNSHIAL 2014(33);
623 LVDnNTWNNSH 2009(64);
623 ANRTTLVDnNTW 2014(33);
623 MANRTTLVDnNTW 2014(33);
623 TTLVDnNTWNNSH 2014(33); 2015(unpublished);
623 VDnNTWNNSHIAL 2014(33);
623 TTLVDnNTWNNSHIA 2014(33);
623 IAGMANRTTLVDnNTW 2014(33);
623 TTLVDnNTWNNSHIAL 2014(33);
623 QIAGMANRTTLVDnNTW 2014(33);
623 TLVDnNTWNNSHIALVGK 2014(33); 2015(unpublished);
623 VDnNTWNNSHIALVGKAM 2014(33);
623 TTIVDnNTWNNSHIAIVGK 2013(43); 2014(16); 2014(32);
623 TTLVDnNTWNNSHIALVGK 2011(58); 2012(48); 2013(34); 2013(34); 2013(34); 2014(13); 2014(15); 2014(17); 2014(18); 2014(22); 2014(25); 2014(33); 2015(1); 2015(2); 2015(9); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2007;2007;2007;
623 NRTTLVDnNTWNNSHIALVGK 2014(33);
623 QIAGMANRTTLVDnNTWNNSHIAL 2014(33);
623 VDnNTWNNSHIALVGKAMSSNETAAY 2014(33);
627 nNSHIAL 2014(33);
627 nNSHIALVGK 2015(1);
627 LVDNNTWnNSH 2009(64);
627 TTLVDNNTWnNSH 2014(33); 2015(unpublished);
627 VDNNTWnNSHIAL 2014(33);
627 TTLVDNNTWnNSHIA 2014(33);
627 TTLVDNNTWnNSHIAL 2014(33);
627 TLVDNNTWnNSHIALVGK 2014(33); 2015(unpublished);
627 VDNNTWnNSHIALVGKAM 2014(33);
627 TTIVDNNTWnNSHIAIVGK 2013(43); 2014(16); 2014(32);
627 TTLVDNNTWnNSHIALVGK 2011(58); 2012(48); 2013(34); 2013(34); 2013(34); 2014(13); 2014(15); 2014(17); 2014(18); 2014(22); 2014(25); 2014(33); 2015(1); 2015(2); 2015(9); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2007;2007;2007;
627 NRTTLVDNNTWnNSHIALVGK 2014(33);
627 QIAGMANRTTLVDNNTWnNSHIAL 2014(33);
627 VDNNTWnNSHIALVGKAMSSNETAAY 2014(33);
641 SSnETAAYK 2014(33); 2015(unpublished);
641 MSSnETAAYK 2012(48);
641 AMSSnETAAYK 2007(75); 2011(54); 2012(48); 2012(50); 2013(34); 2013(34); 2013(34); 2013(40); 2014(13); 2014(15); 2014(16); 2014(17); 2014(18); 2014(19); 2014(22); 2014(32); 2014(32); 2014(33); 2015(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015;2015;2015;2007;2007;
641 GKAMSSnETAAYK 2013(37);
641 VGKAMSSnETAAY 2014(33);
641 AMSSnETAAYKIMR 2011(58);
641 VDNNTWNNSHIALVGKAMSSnETAAY 2014(33);

Sequence

1112131415161718191
MAEPSAPESKHKSSLNSSPWSGLMALGNSRHGHHGPGAQCAHKAAGGAAPPKPAPAGLSGGLSQPAGWQSLLSFTILFLAWLAGFSSRLFAVIRFESIIH
101111121131141151161171181191
EFDPWFNYRSTHHLASHGFYEFLNWFDERAWYPLGRIVGGTVYPGLMITAGLIHWILNTLNITVHIRDVCVFLAPTFSGLTSISTFLLTRELWNQGAGLL
201211221231241251261271281291
AACFIAIVPGYISRSVAGSFDNEGIAIFALQFTYYLWVKSVKTGSVFWTMCCCLSYFYMVSAWGGYVFIINLIPLHVFVLLLMQRYSKRVYIAYSTFYIV
301311321331341351361371381391
GLILSMQIPFVGFQPIRTSEHMAAAGVFALLQAYAFLQYLRDRLTKQEFQTLFFLGVSLAAGAVFLSVIYLTYTGYIAPWSGRFYSLWDTGYAKIHIPII
401411421431441451461471481491
ASVSEHQPTTWVSFFFDLHILVCTFPAGLWFCIKNINDERVFVALYAISAVYFAGVMVRLMLTLTPVVCMLSAIAFSNVFEHYLGDDMKRENPPVEDSSD
501511521531541551561571581591
EDDKRNQGNLYDKAGKVRKHATEQEKTEEGLGPNIKSIVTMLMLMLLMMFAVHCTWVTSNAYSSPSVVLASYNHDGTRNILDDFREAYFWLRQNTDEHAR
601611621631641651661671681691
VMSWWDYGYQIAGMANRTTLVDNNTWNNSHIALVGKAMSSNETAAYKIMRTLDVDYVLVIFGGVIGYSGDDINKFLWMVRIAEGEHPKDIRESDYFTPQG
701711721731741751761771781791
EFRVDKAGSPTLLNCLMYKMSYYRFGEMQLDFRTPPGFDRTRNAEIGNKDIKFKHLEEAFTSEHWLVRIYKVKAPDNRETLDHKPRVTNIFPKQKYLSKK
801811821
TTKRKRGYIKNKLVFKKGKKISKKTV

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
9Smeekens JM, Chen WX, Wu RH: Mass Spectrometric Analysis of the Cell Surface N-Glycoproteome by Combining Metabolic Labeling and Click Chemistry. Journal of the American Society for Mass Spectrometry 2015, 26:604-614.
12Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
15Chen R, Seebun D, Ye M, Zou H, Figeys D: Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS. Journal of Proteomics 2014, 103:194-203.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
17Fang C, Xiong Z, Qin H, Huang G, Liu J, Ye M, Feng S, Zou H: One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides. Analytica Chimica Acta 2014, 841:99-105.
18Hirao Y, Matsuzaki H, Iwaki J, Kuno A, Kaji H, Ohkura T, Togayachi A, Abe M, Nomura M, Noguchi M, et al: Glycoproteomics Approach for Identifying Glycobiomarker Candidate Molecules for Tissue Type Classification of Non-small Cell Lung Carcinoma. Journal of Proteome Research 2014, 13:4705-4716.
19Huang G, Sun Z, Qin H, Zhao L, Xiong Z, Peng X, Ou J, Zou H: Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides. Analyst 2014, 139:2199-2206.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
25Sun Z, Dong J, Zhang S, Hu Z, Cheng K, Li K, Xu B, Ye M, Nie Y, Fan D, Zou H: Identification of Chemoresistance-Related Cell-Surface Glycoproteins in Leukemia Cells and Functional Validation of Candidate Glycoproteins. Journal of Proteome Research 2014, 13:1593-1601.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
43Wang GG, Wu YB, Zhou T, Guo YS, Zheng B, Wang J, Bi Y, Liu FJ, Zhou ZM, Guo XJ, Sha JH: Mapping of the N-Linked Glycoproteome of Human Spermatozoa. Journal of Proteome Research 2013, 12:5750-5759.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
50Whitmore TE, Peterson A, Holzman T, Eastham A, Amon L, McIntosh M, Ozinsky A, Nelson PS, Martin DB: Integrative Analysis of N-Linked Human Glycoproteomic Data Sets Reveals PTPRF Ectodomain as a Novel Plasma Biomarker Candidate for Prostate Cancer. Journal of Proteome Research 2012, 11:2653-2665.
54Tian Y, Yao Z, Roden R, Zhang H: Identification of glycoproteins associated with different histological subtypes of ovarian tumors using quantitative glycoproteomics. Proteomics 2011, 11:4677-4687.
58Nagano K, Shinkawa T, Kato K, Inomata N, Yabuki N, Haramura M: Distinct cell surface proteome profiling by biotin labeling and glycoprotein capturing. Journal of Proteomics 2011, 74:1985-1993.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
75Sun B, Ranish JA, Utleg AG, White JT, Yan X, Lin B, Hood L: Shotgun glycopeptide capture approach coupled with mass Spectrometry for comprehensive glycoproteomics. Molecular & Cellular Proteomics 2007, 6:141-149.