Contains      

UniProtKB-Q8TEM1

Protein Nuclear pore membrane glycoprotein 210
Gene NUP210
Status Reviewed
Source 22Rv1 cell xenograft (prostate cancer); Breast Cancer cell lines; Breast cancer xenografts; Breast tumor; Colorectal cancer cell line, HCT-116 (colorectal cancer); Colorectal tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); HCC cell lines (Liver); HEK293 cell line (embryonic kidney); HEK293T cell line (embryonic kidney); HEK293T cell line (embryonic kidney), HEK293T cell line (embryonic kidney); Hela cell line (cervical cancer); IGROV-1/CP cell line (Ovarian cancer)IGROV-1/CP; Jurkat T cell line; K562A cell line (Leukemia); K562S cell line (Leukemia); LNCap/PC3 cell lines (Prostate cancer); Liver; Liver tumor (HCC); Lymphocytes; OVCAR-3 cell line (Ovarian cancer); Ovarian tumor; Ovarian tumorJurkat T cell line; PBMC Macrophage cells; Pancreatic islets; Prostate; Prostate cancer metastasis to liver; Prostate tumor; SKOV-3 cell line (Ovarian cancer); Spermatozoa; Urine
Years 2007-2015

Glycosites

Site Identified Peptides Year(Publication ID)
44 VnFTLE 2014(33);
44 VnFTIEASEGCYR 2013(43); 2014(16);
44 VnFTLEASEGCYR 2007(75); 2011(57); 2012(48); 2012(50); 2014(13); 2014(14); 2014(15); 2014(17); 2014(33); 2015(unpublished);2015(1); 2015(2); 2015(unpublished);2015(unpublished);2015(12);
337 LPnSTIYVVE 2014(33);
337 QGASRLPnSTIY 2014(33);
337 MQGASRLPnSTIY 2014(33);
337 nSTIYVVEPGYLGFTVHPGDR 2015(unpublished);
337 IPnSTIYVVEPGYIGFTVHPGDR 2014(32);
337 LPnSTIYVVEPGYLGFTVHPGDR 2007(75); 2007(58); 2011(48); 2012(50); 2012(34); 2013(34); 2013(13); 2014(15); 2014(33); 2014(1); 2015(2); 2015(9); 2015(12); 2015(unpublished);2015(unpublished);2015(Unpublished ); 2007;2007;2007 ;
337 MQGASRIPnSTIYVVEPGYIGFTVHPGDR 2014(16);
337 IPnSTIYVVEPGYIGFTVHPGDRWVIETGR 2013(43);
337 LPnSTIYVVEPGYLGFTVHPGDRWVLETGR 2015(2);
405 SSSQnGSYHR 2014(33);
405 VLSSSQnGSYHR 2014(33);
405 EVLSSSQnGSYHR 2014(33);
405 IETVIPAEFFEVISSSQnGSYHR 2013(43); 2014(16); 2014(32); 2014(32);
405 IETVLPAEFFEVLSSSQnGSYHR 2007(75); 2007(64); 2009(57); 2011(58); 2011(50); 2012(34); 2013(34); 2013(34); 2013(13); 2014(15); 2014(25); 2014(25); 2014(33); 2014(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015(Unpublished ); 2007;2007;2007 ;
405 VYVSDNIRIETVLPAEFFEVLSSSQnGSYHR 2015(2);
484 TIRAHGGSGnF 2014(33);
484 AHGGSGnFSWSSSSHIVATVTVK 2014(16);
484 AHGGSGnFSWSSSSHLVATVTVK 2015(2);
681 FQnVTAEDTDSIGL 2014(33);
681 FFQnVTAEDTDSIGL 2014(33);
681 FQnVTAEDTDSIGLALF 2014(33);
681 FQnVTAEDTDSIGLALFAPH 2014(33);
681 FFQnVTAEDTDSIGLALFAPH 2015(unpublished);
681 FQnVTAEDTDSIGLALFAPHSSR 2014(33);
681 FFQnVTAEDTDSIGIAIFAPHSSR 2013(43); 2014(16); 2014(32); 2014(32);
681 FFQnVTAEDTDSIGLALFAPHSSR 2011(58); 2014(15); 2014(33); 2015(unpublished);2015(1); 2015(2); 2015(unpublished);
681 EMLFEGGPRPWILEPSKFFQnVTAEDTDSIGLALFAPHSSR 2015(2);
801 RFDnFSSL 2011(57); 2014(33);
801 RFDnFSSLSIQW 2011(57);
801 RFDnFSSLSIQWE 2014(33);
801 RFDnFSSLSIQWESTR 2015(unpublished);
801 FDnFSSLSIQWESTRPVLASIEPELPMQLVSQDDESGQK 2015(2);
801 RFDnFSSISIQWESTRPVIASIEPEIPMQIVSQDDESGQK 2013(43);
801 RFDnFSSLSIQWESTRPVLASIEPELPMQLVSQDDESGQK 2015(unpublished);2015(2);
926 nTSTADVVK 2015(unpublished);2015(1);
926 FLnTSTADVVK 2011(57); 2012(48); 2014(33); 2015(unpublished);
926 FFLnTSTADVVK 2014(33);
926 FLnTSTADVVKVAY 2014(33);
926 FFLnTSTADVVKVAY 2014(33);
926 GSGYFFLnTSTADVVK 2014(33);
926 EGSGYFFInTSTADVVK 2014(32); 2014(32);
926 EGSGYFFLnTSTADVVK 2007(75); 2009(64); 2011(57); 2011(58); 2012(48); 2013(34); 2013(34); 2014(13); 2014(15); 2014(22); 2014(33); 2015(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2007(unpublished);2007;2007;
926 IREGSGYFFInTSTADVVK 2013(43); 2014(16);
926 IREGSGYFFLnTSTADVVK 2011(58); 2013(34); 2014(15); 2014(33); 2015(1); 2015(2);
1039 ALDnYTITF 2014(33);
1039 VALDEALDnY 2014(33);
1039 ALDnYTITFLIR 2014(33);
1039 VALDEALDnYTITF 2014(33);
1039 ALDEALDnYTITFLIR 2007(unpublished);2007 (Unpublished );
1039 AASPIITIVAIDEAIDnYTITFIIR 2013(43);
1039 AASPIITLVALDEALDnYTITFLIR 2014(13); 2014(15); 2014(33); 2015(unpublished);2015(2);
1116 GGPQPQSNILFSISnE 2014(33);
1135 AIGnGTVSGL 2014(33);
1135 AIGnGTVSGLVQAVDAETGK 2015(unpublished);
1362 nQTIIVAVK 2015(1);
1362 GAnQTIIVAVK 2014(33);
1362 VIAQEPFGAnQT 2014(33);
1362 GAnQTIIVAVKVSPVSY 2014(33);
1362 GAnQTIIVAVKVSPVSYL 2014(33);
1362 VIAQEPFGAnQTIIVAVK 2014(33);
1362 GFLASGSMIGTSTIEVIAQEPFGAnQTIIVAVK 2015(2);
1441 nNTCVVR 2015(1);
1441 GPTnNTCVVR 2007(75); 2011(58); 2012(48); 2013(34); 2013(40); 2014(13); 2014(15); 2014(16); 2014(17); 2014(22); 2014(32); 2014(33); 2015(unpublished);2015(1); 2015(2); 2015(unpublished);2015(unpublished);2015(12);
1441 IGKGPTnNTC 2009(64);
1441 IGKGPTnNTCVVR 2013(37);
1441 VQIGKGPTnNTCVVR 2014(33);
1441 VQIGKGPTnNTCVVRT 2014(33);
1441 DDFVQIGKGPTnNTCVVR 2013(43); 2014(13); 2015(12);
1441 VQIGKGPTnNTCVVRTVSVGL 2014(33);
1441 ATNRDDFVQIGKGPTnNTCVVR 2014(33);

Sequence

1112131415161718191
MAARGRGLLLLTLSVLLAAGPSAAAAKLNIPKVLLPFTRATRVNFTLEASEGCYRWLSTRPEVASIEPLGLDEQQCSQKAVVQARLTQPARLTSIIFAED
101111121131141151161171181191
ITTGQVLRCDAIVDLIHDIQIVSTTRELYLEDSPLELKIQALDSEGNTFSTLAGLVFEWTIVKDSEADRFSDSHNALRILTFLESTYIPPSYISEMEKAA
201211221231241251261271281291
KQGDTILVSGMKTGSSKLKARIQEAVYKNVRPAEVRLLILENILLNPAYDVYLMVGTSIHYKVQKIRQGKITELSMPSDQYELQLQNSIPGPEGDPARPV
301311321331341351361371381391
AVLAQDTSMVTALQLGQSSLVLGHRSIRMQGASRLPNSTIYVVEPGYLGFTVHPGDRWVLETGRLYEITIEVFDKFSNKVYVSDNIRIETVLPAEFFEVL
401411421431441451461471481491
SSSQNGSYHRIRALKRGQTAIDAALTSVVDQDGGVHILQVPVWNQQEVEIHIPITLYPSILTFPWQPKTGAYQYTIRAHGGSGNFSWSSSSHLVATVTVK
501511521531541551561571581591
GVMTTGSDIGFSVIQAHDVQNPLHFGEMKVYVIEPHSMEFAPCQVEARVGQALELPLRISGLMPGGASEVVTLSDCSHFDLAVEVENQGVFQPLPGRLPP
601611621631641651661671681691
GSEHCSGIRVKAEAQGSTTLLVSYRHGHVHLSAKITIAAYLPLKAVDPSSVALVTLGSSKEMLFEGGPRPWILEPSKFFQNVTAEDTDSIGLALFAPHSS
701711721731741751761771781791
RNYQQHWILVTCQALGEQVIALSVGNKPSLTNPFPAVEPAVVKFVCAPPSRLTLAPVYTSPQLDMSCPLLQQNKQVVPVSSHRNPRLDLAAYDQEGRRFD
801811821831841851861871881891
NFSSLSIQWESTRPVLASIEPELPMQLVSQDDESGQKKLHGLQAILVHEASGTTAITATATGYQESHLSSARTKQPHDPLVPLSASIELILVEDVRVSPE
901911921931941951961971981991
EVTIYNHPGIQAELRIREGSGYFFLNTSTADVVKVAYQEARGVAMVHPLLPGSSTIMIHDLCLVFPAPAKAVVYVSDIQELYIRVVDKVEIGKTVKAYVR
1001101110211031104110511061107110811091
VLDLHKKPFLAKYFPFMDLKLRAASPIITLVALDEALDNYTITFLIRGVAIGQTSLTASVTNKAGQRINSAPQQIEVFPPFRLMPRKVTLLIGATMQVTS
1101111111211131114111511161117111811191
EGGPQPQSNILFSISNESVALVSAAGLVQGLAIGNGTVSGLVQAVDAETGKVVIISQDLVQVEVLLLRAVRIRAPIMRMRTGTQMPIYVTGITNHQNPFS
1201121112211231124112511261127112811291
FGNAVPGLTFHWSVTKRDVLDLRGRHHEASIRLPSQYNFAMNVLGRVKGRTGLRVVVKAVDPTSGQLYGLARELSDEIQVQVFEKLQLLNPEIEAEQILM
1301131113211331134113511361137113811391
SPNSYIKLQTNRDGAASLSYRVLDGPEKVPVVHVDEKGFLASGSMIGTSTIEVIAQEPFGANQTIIVAVKVSPVSYLRVSMSPVLHTQNKEALVAVPLGM
1401141114211431144114511461147114811491
TVTFTVHFHDNSGDVFHAHSSVLNFATNRDDFVQIGKGPTNNTCVVRTVSVGLTLLRVWDAEHPGLSDFMPLPVLQAISPELSGAMVVGDVLCLATVLTS
1501151115211531154115511561157115811591
LEGLSGTWSSSANSILHIDPKTGVAVARAVGSVTVYYEVAGHLRTYKEVVVSVPQRIMARHLHPIQTSFQEATASKVIVAVGDRSSNLRGECTPTQREVI
1601161116211631164116511661167116811691
QALHPETLISCQSQFKPAVFDFPSQDVFTVEPQFDTALGQYFCSITMHRLTDKQRKHLSMKKTALVVSASLSSSHFSTEQVGAEVPFSPGLFADQAEILL
1701171117211731174117511761177117811791
SNHYTSSEIRVFGAPEVLENLEVKSGSPAVLAFAKEKSFGWPSFITYTVGVLDPAAGSQGPLSTTLTFSSPVTNQAIAIPVTVAFVVDRRGPGPYGASLF
180118111821183118411851186118711881
QHFLDSYQVMFFTLFALLAGTAVMIIAYHTVCTPRDLAVPAALTPRASPGHSPHYFAASSPTSPNALPPARKASPPSGLWSPAYASH

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
9Smeekens JM, Chen WX, Wu RH: Mass Spectrometric Analysis of the Cell Surface N-Glycoproteome by Combining Metabolic Labeling and Click Chemistry. Journal of the American Society for Mass Spectrometry 2015, 26:604-614.
12Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
15Chen R, Seebun D, Ye M, Zou H, Figeys D: Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS. Journal of Proteomics 2014, 103:194-203.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
17Fang C, Xiong Z, Qin H, Huang G, Liu J, Ye M, Feng S, Zou H: One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides. Analytica Chimica Acta 2014, 841:99-105.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
25Sun Z, Dong J, Zhang S, Hu Z, Cheng K, Li K, Xu B, Ye M, Nie Y, Fan D, Zou H: Identification of Chemoresistance-Related Cell-Surface Glycoproteins in Leukemia Cells and Functional Validation of Candidate Glycoproteins. Journal of Proteome Research 2014, 13:1593-1601.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
43Wang GG, Wu YB, Zhou T, Guo YS, Zheng B, Wang J, Bi Y, Liu FJ, Zhou ZM, Guo XJ, Sha JH: Mapping of the N-Linked Glycoproteome of Human Spermatozoa. Journal of Proteome Research 2013, 12:5750-5759.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
50Whitmore TE, Peterson A, Holzman T, Eastham A, Amon L, McIntosh M, Ozinsky A, Nelson PS, Martin DB: Integrative Analysis of N-Linked Human Glycoproteomic Data Sets Reveals PTPRF Ectodomain as a Novel Plasma Biomarker Candidate for Prostate Cancer. Journal of Proteome Research 2012, 11:2653-2665.
57Chen Y, Cao J, Yan G, Lu H, Yang P: Two-step protease digestion and glycopeptide capture approach for accurate glycosite identification and glycoprotein sequence coverage improvement. Talanta 2011, 85:70-75.
58Nagano K, Shinkawa T, Kato K, Inomata N, Yabuki N, Haramura M: Distinct cell surface proteome profiling by biotin labeling and glycoprotein capturing. Journal of Proteomics 2011, 74:1985-1993.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
75Sun B, Ranish JA, Utleg AG, White JT, Yan X, Lin B, Hood L: Shotgun glycopeptide capture approach coupled with mass Spectrometry for comprehensive glycoproteomics. Molecular & Cellular Proteomics 2007, 6:141-149.