Contains      

UniProtKB-Q92542

Protein Nicastrin
Gene NCSTN
Status Reviewed
Source 22Rv1 cell xenograft (prostate cancer); Breast Cancer cell lines; Breast cancer cell lines; Breast cancer xenografts; Breast cell lines; Breast cell linesJurkat T cell line; Breast tumor; Colorectal cancer cell line, HCT-116 (colorectal cancer); DLBCL cell lines (Diffuse large B-cell lymphoma); HCC cell lines (Liver); HCC cell lines (Liver)Jurkat T cell line; HEK293 cell line (embryonic kidney); HEK293T cell line (embryonic kidney); HEK293T cell line (embryonic kidney), HEK293T cell line (embryonic kidney); Hela cell line (cervical cancer); IGROV-1/CP cell line (Ovarian cancer)IGROV-1/CP; Jurkat T cell line; K562A cell line (Leukemia); K562S cell line (Leukemia); LNCap/PC3 cell lines (Prostate cancer); Liver; Liver tumor (HCC); LnCap cell line (Prostate cancer); Lymphocytes; OVCAR-3 cell line (Ovarian cancer); OVCAR-3 cell line (Ovarian cancer)Jurkat T cell line; Ovarian tumor; Ovarian tumorJurkat T cell line; Pancreatic islets; Platelet; Prostate; Prostate cancer cell lines; Prostate tumor; SKOV-3 cell line (Ovarian cancer); Spermatozoa; Urine; ovarian tumor; prostate cancer cell lines
Years 2003-2015

Glycosites

Site Identified Peptides Year(Publication ID)
45 IYIPInK 2013(43);
45 IYIPLnK 2012(48); 2012(50); 2013(40); 2014(14); 2014(15); 2014(19); 2014(33); 2015(unpublished);2015(2); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);
45 KIYIPInK 2014(16);
45 KIYIPLnK 2007(48); 2012(34); 2013(34); 2013(34); 2013(13); 2014(15); 2014(33); 2014(2); 2015(unpublished);2015(unpublished);2015;2015;2015;2007;2007;
45 IPLnKTAPC 2014(33);
45 IYIPLnKTAPCVR 2011(58); 2015(12);
45 KIYIPLnKTAPCVR 2011(58); 2014(33);
45 RKIYIPLnKTAPCVRL 2009(64);
55 LLnATHQIG 2011(57);
55 LLnATHQIGCQ 2012(48); 2014(33); 2007(unpublished);2007(unpublished);2007(unpublished);
55 LLnATHQIGCQS 2012(48);
55 nATHQIGCQSSISGDTGVIH 2014(33);
55 LLnATHQIGCQSSISGDTGVI 2012(48);
55 LLnATHQIGCQSSISGDTGVIH 2012(48);
55 LLnATHQIGCQSSISGDTGVIHVVE 2014(33);
55 IInATHQIGCQSSISGDTGVIHVVEK 2013(43); 2014(16); 2014(32);
55 LLnATHQIGCQSSISGDTGVIHVVEK 2011(57); 2012(48); 2012(52); 2014(33); 2015(unpublished);2015(1); 2015(unpublished);2015(9);
55 LLnATHQIGCQSSISGDTGVIHVVEKEE 2014(33);
187 LLEDEnETK 2012(48);
187 LLEDEnETKV 2009(64);
187 LLEDEnETKVIKQ 2014(33);
187 SFPIFLLEDEnETK 2014(33);
187 DFSFPIFLLEDEnETK 2014(33); 2007(unpublished);2007(unpublished);
187 GLAYEDFSFPIFLLEDEnETK 2011(57);
187 SLGNGLAYEDFSFPIFLLEDEnETK 2015(unpublished);
187 EIQWNSLGNGLAYEDFSFPIFLLEDEnETK 2011(57); 2014(25); 2014(25); 2015(2);
187 EIQWNSLGNGLAYEDFSFPIFLLEDEnETKVIK 2014(15); 2015(2);
200 QDHnLSQNGSAPTFPL 2014(33);
200 QCYQDHnLSQNGSAPTFPL 2014(33);
200 QCYQDHnLSQNGSAPTFPLCAM 2014(33);
204 QDHNLSQnGSAPTFPL 2014(33);
204 QCYQDHNLSQnGSAPTFPL 2014(33);
204 QCYQDHNLSQnGSAPTFPLCAM 2014(33);
264 nTTGTLKPDDR 2012(48); 2015(1);
264 MIKPInTTGTIK 2013(37);
264 SMLKPInTTGTL 2014(33);
264 PInTTGTIKPDDR 2014(16);
264 PInTTGTLKPDDR 2011(57); 2012(48); 2014(33); 2015(unpublished);
264 SMIKPInTTGTIK 2013(37);
264 IKPInTTGTIKPDDR 2013(43);
264 LKPInTTGTLKPDDR 2012(48); 2014(33); 2015(unpublished);
264 SMLKPInTTGTLKPDDR 2014(33); 2015(unpublished);2007(unpublished);2007(unpublished);2007;
264 SMLKPInTTGTLKPDDRVVV 2014(33);
264 PLSDYNVWSMLKPInTTGTLKPDDR 2007(75);
264 IVCDPLSDYNVWSMLKPInTTGTLKPDDR 2014(33);
264 SINPEIVCDPLSDYNVWSMLKPInTTGTLKPDDR 2015(unpublished);
264 SSIQSTFSINPEIVCDPISDYNVWSMIKPInTTGTIKPDDR 2013(43);
387 MHTDPVSQKnES 2009(64);
387 TDPVSQKnESVR 2015(unpublished);
387 LWMHTDPVSQKnE 2014(33);
387 MHTDPVSQKnESVR 2014(33);
387 LWMHTDPVSQKnESVR 2014(33);
387 nESVRNQVEDLLATLEK 2011(57); 2015(12);
387 TSIEIWMHTDPVSQKnESVR 2013(43); 2014(16);
387 TSLELWMHTDPVSQKnESVR 2003( 83); 2011(58); 2012(52); 2013(34); 2014(13); 2014(33); 2015(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015;2007;2007;2007;
417 RRPnQSQPLPPSSL 2014(33);
417 RPnQSQPIPPSSIQR 2014(16);
417 RPnQSQPLPPSSLQR 2007(72); 2011(57); 2012(48); 2012(52); 2014(13); 2014(33); 2015(unpublished);2015(1); 2015(unpublished);2015(12);
417 SGAGVPAVILRRPnQSQPLPPSSLQR 2011(58);
435 nISGVVLADHSGAF 2014(33);
435 nISGVVIADHSGAFHNK 2013(43);
435 nISGVVLADHSGAFHNK 2007(57); 2011(58); 2011(52); 2012(34); 2013(40); 2013(13); 2014(14); 2014(33); 2014(1); 2015(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015;2015;2007;2007;
435 ARnISGVVLADHSGAFHN 2011(57);
435 ARnISGVVIADHSGAFHNK 2014(16);
435 ARnISGVVLADHSGAFHNK 2011(57); 2012(52); 2013(34); 2014(13); 2014(33); 2015(1); 2015(unpublished);2015(2); 2015(12);
464 NInVSYPE 2014(33);
464 YYQSIYDTAENInVSYPEWISPEEDINFVTDTAK 2013(43); 2014(32);
464 YYQSIYDTAENInVSYPEWLSPEEDLNFVTDTAK 2011(57); 2014(25); 2015(unpublished);2015(2);
506 nFSDTVQADPQTVTR 2015(1);
506 LAGGTnFSDTVQADPQ 2014(33);
506 LAGGTnFSDTVQADPQTVTR 2014(33);
506 ELAGGTnFSDTVQADPQTVTR 2014(33);
506 AIYEIAGGTnFSDTVQADPQTVTR 2013(43);
506 ALYELAGGTnFSDTVQADPQTVTR 2011(54); 2012(48); 2012(50); 2012(52); 2013(34); 2014(14); 2014(25); 2014(33); 2015(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015;2015;2015;2007;2007;2007;
530 IKAnNSWF 2014(33);
530 AnNSWFQSIIR 2013(43); 2014(16);
530 AnNSWFQSILR 2007(57); 2011(58); 2011(48); 2012(50); 2012(52); 2012(13); 2014(15); 2014(19); 2014(25); 2014(25); 2014(33); 2014(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015;2015;2015;2015;2007;2007;
530 GFLIKAnNSWF 2014(33);
530 GFIIKAnNSWFQS 2013(37);
562 IAVSSPTnTTY 2014(33);
562 IAVSSPTnTTYVVQY 2014(33);
562 SYIGDGPIQHYIAVSSPTnTTYVVQYAIANITGTVVNITR 2013(43);
562 SYLGDGPLQHYIAVSSPTnTTYVVQYALANLTGTVVNLTR 2011(58); 2015(1); 2015(2); 2015(9);
573 nLTGTVVNLTR 2015(1);
573 ALAnLTGTVVNL 2014(33);
573 LAnLTGTVVNLTR 2007(unpublished);2007(unpublished);2007(unpublished);
573 ALAnLTGTVVNLTR 2011(57); 2014(33); 2015(unpublished);
573 VVQYALAnLTGTVVNLTR 2011(57); 2014(33);
573 SYIGDGPIQHYIAVSSPTNTTYVVQYAIAnITGTVVNITR 2013(43);
573 SYLGDGPLQHYIAVSSPTNTTYVVQYALAnLTGTVVNLTR 2011(58); 2015(1); 2015(2); 2015(9);
580 GTVVnLTR 2014(33); 2015(unpublished);
580 NLTGTVVnLTR 2015(1);
580 ALANLTGTVVnL 2014(33);
580 LANLTGTVVnLTR 2007(unpublished);2007(unpublished);2007(unpublished);
580 ALANLTGTVVnLTR 2011(57); 2014(33); 2015(unpublished);
580 VVQYALANLTGTVVnLTR 2011(57); 2014(33);
580 SYIGDGPIQHYIAVSSPTNTTYVVQYAIANITGTVVnITR 2013(43);
580 SYLGDGPLQHYIAVSSPTNTTYVVQYALANLTGTVVnLTR 2011(58); 2015(1); 2015(2); 2015(9);
612 nETDRLPR 2015(1);
612 YSWVQGPLHSnE 2014(33);

Sequence

1112131415161718191
MATAGGGSGADPGSRGLLRLLSFCVLLAGLCRGNSVERKIYIPLNKTAPCVRLLNATHQIGCQSSISGDTGVIHVVEKEEDLQWVLTDGPNPPYMVLLES
101111121131141151161171181191
KHFTRDLMEKLKGRTSRIAGLAVSLTKPSPASGFSPSVQCPNDGFGVYSNSYGPEFAHCREIQWNSLGNGLAYEDFSFPIFLLEDENETKVIKQCYQDHN
201211221231241251261271281291
LSQNGSAPTFPLCAMQLFSHMHAVISTATCMRRSSIQSTFSINPEIVCDPLSDYNVWSMLKPINTTGTLKPDDRVVVAATRLDSRSFFWNVAPGAESAVA
301311321331341351361371381391
SFVTQLAAAEALQKAPDVTTLPRNVMFVFFQGETFDYIGSSRMVYDMEKGKFPVQLENVDSFVELGQVALRTSLELWMHTDPVSQKNESVRNQVEDLLAT
401411421431441451461471481491
LEKSGAGVPAVILRRPNQSQPLPPSSLQRFLRARNISGVVLADHSGAFHNKYYQSIYDTAENINVSYPEWLSPEEDLNFVTDTAKALADVATVLGRALYE
501511521531541551561571581591
LAGGTNFSDTVQADPQTVTRLLYGFLIKANNSWFQSILRQDLRSYLGDGPLQHYIAVSSPTNTTYVVQYALANLTGTVVNLTREQCQDPSKVPSENKDLY
601611621631641651661671681691
EYSWVQGPLHSNETDRLPRCVRSTARLARALSPAFELSQWSSTEYSTWTESRWKDIRARIFLIASKELELITLTVGFGILIFSLIVTYCINAKADVLFIA
701
PREPGAVSY

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
9Smeekens JM, Chen WX, Wu RH: Mass Spectrometric Analysis of the Cell Surface N-Glycoproteome by Combining Metabolic Labeling and Click Chemistry. Journal of the American Society for Mass Spectrometry 2015, 26:604-614.
12Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
15Chen R, Seebun D, Ye M, Zou H, Figeys D: Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS. Journal of Proteomics 2014, 103:194-203.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
19Huang G, Sun Z, Qin H, Zhao L, Xiong Z, Peng X, Ou J, Zou H: Preparation of hydrazine functionalized polymer brushes hybrid magnetic nanoparticles for highly specific enrichment of glycopeptides. Analyst 2014, 139:2199-2206.
25Sun Z, Dong J, Zhang S, Hu Z, Cheng K, Li K, Xu B, Ye M, Nie Y, Fan D, Zou H: Identification of Chemoresistance-Related Cell-Surface Glycoproteins in Leukemia Cells and Functional Validation of Candidate Glycoproteins. Journal of Proteome Research 2014, 13:1593-1601.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
43Wang GG, Wu YB, Zhou T, Guo YS, Zheng B, Wang J, Bi Y, Liu FJ, Zhou ZM, Guo XJ, Sha JH: Mapping of the N-Linked Glycoproteome of Human Spermatozoa. Journal of Proteome Research 2013, 12:5750-5759.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
50Whitmore TE, Peterson A, Holzman T, Eastham A, Amon L, McIntosh M, Ozinsky A, Nelson PS, Martin DB: Integrative Analysis of N-Linked Human Glycoproteomic Data Sets Reveals PTPRF Ectodomain as a Novel Plasma Biomarker Candidate for Prostate Cancer. Journal of Proteome Research 2012, 11:2653-2665.
52Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667.
54Tian Y, Yao Z, Roden R, Zhang H: Identification of glycoproteins associated with different histological subtypes of ovarian tumors using quantitative glycoproteomics. Proteomics 2011, 11:4677-4687.
57Chen Y, Cao J, Yan G, Lu H, Yang P: Two-step protease digestion and glycopeptide capture approach for accurate glycosite identification and glycoprotein sequence coverage improvement. Talanta 2011, 85:70-75.
58Nagano K, Shinkawa T, Kato K, Inomata N, Yabuki N, Haramura M: Distinct cell surface proteome profiling by biotin labeling and glycoprotein capturing. Journal of Proteomics 2011, 74:1985-1993.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
72Hanson SR, Hsu T-L, Weerapana E, Kishikawa K, Simon GM, Cravatt BF, Wong C-H: Tailored glycoproteomics and glycan site mapping using saccharide-selective bioorthogonal probes. Journal of the American Chemical Society 2007, 129:7266-+.
75Sun B, Ranish JA, Utleg AG, White JT, Yan X, Lin B, Hood L: Shotgun glycopeptide capture approach coupled with mass Spectrometry for comprehensive glycoproteomics. Molecular & Cellular Proteomics 2007, 6:141-149.