Contains      

UniProtKB-Q96D15

Protein Reticulocalbin-3
Gene RCN3
Status Reviewed
Source Breast Cancer cell lines; IGROV-1/CP cell line (Ovarian cancer)IGROV-1/CP; LNCap/PC3 cell lines (Prostate cancer); Liver; Ovarian tumor; umbilical vein Endothelial Cells
Years 2007-2015

Glycosites

Site Identified Peptides Year(Publication ID)
140 EELRnATY 2014(33);
140 nATYGHYAPGEE 2014(33);
140 GWEEIRnATYGHY 2013(37);
140 nATYGHYAPGEEF 2014(33);
140 nATYGHYAPGEEFH 2007(75);
140 nATYGHYAPGEEFHDVE 2014(33);
140 DTDRDGRVGWEELRnATY 2014(33);
140 nATYGHYAPGEEFHDVEDAE 2014(33);
140 nATYGHYAPGEEFHDVEDAETY 2015(unpublished);
140 nATYGHYAPGEEFHDVEDAETYK 2009(64); 2013(34); 2013(44); 2014(33); 2015(unpublished);2015(2);
140 nATYGHYAPGEEFHDVEDAETYKK 2007(75); 2013(34); 2014(33); 2015(unpublished);2015(2);
140 RnATYGHYAPGEEFHDVEDAETYK 2015(unpublished);
140 VGWEELRnATYGHYAPGEEFHDVEDAETYKK 2015(2);

Sequence

1112131415161718191
MMWRPSVLLLLLLLRHGAQGKPSPDAGPHGQGRVHQAAPLSDAPHDDAHGNFQYDHEAFLGREVAKEFDQLTPEESQARLGRIVDRMDRAGDGDGWVSLA
101111121131141151161171181191
ELRAWIAHTQQRHIRDSVSAAWDTYDTDRDGRVGWEELRNATYGHYAPGEEFHDVEDAETYKKMLARDERRFRVADQDGDSMATREELTAFLHPEEFPHM
201211221231241251261271281291
RDIVIAETLEDLDRNKDGYVQVEEYIADLYSAEPGEEEPAWVQTERQQFRDFRDLNKDGHLDGSEVGHWVLPPAQDQPLVEANHLLHESDTDKDGRLSKA
301311321
EILGNWNMFVGSQATNYGEDLTRHHDEL

Reference

ID Publication
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
44Yin X, Bern M, Xing Q, Ho J, Viner R, Mayr M: Glycoproteomic Analysis of the Secretome of Human Endothelial Cells. Molecular & Cellular Proteomics 2013, 12:956-978.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
75Sun B, Ranish JA, Utleg AG, White JT, Yan X, Lin B, Hood L: Shotgun glycopeptide capture approach coupled with mass Spectrometry for comprehensive glycoproteomics. Molecular & Cellular Proteomics 2007, 6:141-149.