Detail


Protein	Msx2-interacting protein
Gene	SPEN
Status	Reviewed
Source	Liver; OVCAR-3 cell line (Ovarian cancer); Prostate tumor; Serum
Years	2007-2015

Site	Identified Peptides	Year(Publication ID)
1299	nITVR	2014(33);
1299	nLTVR	2014(33);
1461	SSREEnWSFLDWDSR	2007(unpublished);2007(unpublished);
2894	nASPVISSVK	2015(1);
2894	GPQAPAGYANVATHSTLVLTAQTYnASPVISSVK	2015(10);

Sequence

1	11	21	31	41	51	61	71	81	91
MVRETRHLWV	GNLPENVREE	KIIEHFKRYG	RVESVKILPK	RGSEGGVAAF	VDFVDIKSAQ	KAHNSVNKMG	DRDLRTDYNE	PGTIPSAARG	LDDTVSIASR
101	111	121	131	141	151	161	171	181	191
SREVSGFRGG	GGGPAYGPPP	SLHAREGRYE	RRLDGASDNR	ERAYEHSAYG	HHERGTGGFD	RTRHYDQDYY	RDPRERTLQH	GLYYASRSRS	PNRFDAHDPR
201	211	221	231	241	251	261	271	281	291
YEPRAREQFT	LPSVVHRDIY	RDDITREVRG	RRPERNYQHS	RSRSPHSSQS	RNQSPQRLAS	QASRPTRSPS	GSGSRSRSSS	SDSISSSSST	SSDSSDSSSS
301	311	321	331	341	351	361	371	381	391
SSDDSPARSV	QSAAVPAPTS	QLLSSLEKDE	PRKSFGIKVQ	NLPVRSTDTS	LKDGLFHEFK	KFGKVTSVQI	HGTSEERYGL	VFFRQQEDQE	KALTASKGKL
401	411	421	431	441	451	461	471	481	491
FFGMQIEVTA	WIGPETESEN	EFRPLDERID	EFHPKATRTL	FIGNLEKTTT	YHDLRNIFQR	FGEIVDIDIK	KVNGVPQYAF	LQYCDIASVC	KAIKKMDGEY
501	511	521	531	541	551	561	571	581	591
LGNNRLKLGF	GKSMPTNCVW	LDGLSSNVSD	QYLTRHFCRY	GPVVKVVFDR	LKGMALVLYN	EIEYAQAAVK	ETKGRKIGGN	KIKVDFANRE	SQLAFYHCME
601	611	621	631	641	651	661	671	681	691
KSGQDIRDFY	EMLAERREER	RASYDYNQDR	TYYESVRTPG	TYPEDSRRDY	PARGREFYSE	WETYQGDYYE	SRYYDDPREY	RDYRNDPYEQ	DIREYSYRQR
701	711	721	731	741	751	761	771	781	791
ERERERERFE	SDRDRDHERR	PIERSQSPVH	LRRPQSPGAS	PSQAERLPSD	SERRLYSRSS	DRSGSCSSLS	PPRYEKLDKS	RLERYTKNEK	TDKERTFDPE
801	811	821	831	841	851	861	871	881	891
RVERERRLIR	KEKVEKDKTD	KQKRKGKVHS	PSSQSSETDQ	ENEREQSPEK	PRSCNKLSRE	KADKEGIAKN	RLELMPCVVL	TRVKEKEGKV	IDHTPVEKLK
901	911	921	931	941	951	961	971	981	991
AKLDNDTVKS	SALDQKLQVS	QTEPAKSDLS	KLESVRMKVP	KEKGLSSHVE	VVEKEGRLKA	RKHLKPEQPA	DGVSAVDLEK	LEARKRRFAD	SNLKAEKQKP
1001	1011	1021	1031	1041	1051	1061	1071	1081	1091
EVKKSSPEME	DARVLSKKQP	DVSSREVILL	REGEAERKPV	RKEILKRESK	KIKLDRLNTV	ASPKDCQELA	SISVGSGSRP	SSDLQARLGE	LAGESVENQE
1101	1111	1121	1131	1141	1151	1161	1171	1181	1191
VQSKKPIPSK	PQLKQLQVLD	DQGPEREDVR	KNYCSLRDET	PERKSGQEKS	HSVNTEEKIG	IDIDHTQSYR	KQMEQSRRKQ	QMEMEIAKSE	KFGSPKKDVD
1201	1211	1221	1231	1241	1251	1261	1271	1281	1291
EYERRSLVHE	VGKPPQDVTD	DSPPSKKKRM	DHVDFDICTK	RERNYRSSRQ	ISEDSERTGG	SPSVRHGSFH	EDEDPIGSPR	LLSVKGSPKV	DEKVLPYSNI
1301	1311	1321	1331	1341	1351	1361	1371	1381	1391
TVREESLKFN	PYDSSRREQM	ADMAKIKLSV	LNSEDELNRW	DSQMKQDAGR	FDVSFPNSII	KRDSLRKRSV	RDLEPGEVPS	DSDEDGEHKS	HSPRASALYE
1401	1411	1421	1431	1441	1451	1461	1471	1481	1491
SSRLSFLLRD	REDKLRERDE	RLSSSLERNK	FYSFALDKTI	TPDTKALLER	AKSLSSSREE	NWSFLDWDSR	FANFRNNKDK	EKVDSAPRPI	PSWYMKKKKI
1501	1511	1521	1531	1541	1551	1561	1571	1581	1591
RTDSEGKMDD	KKEDHKEEEQ	ERQELFASRF	LHSSIFEQDS	KRLQHLERKE	EDSDFISGRI	YGKQTSEGAN	STTDSIQEPV	VLFHSRFMEL	TRMQQKEKEK
1601	1611	1621	1631	1641	1651	1661	1671	1681	1691
DQKPKEVEKQ	EDTENHPKTP	ESAPENKDSE	LKTPPSVGPP	SVTVVTLESA	PSALEKTTGD	KTVEAPLVTE	EKTVEPATVS	EEAKPASEPA	PAPVEQLEQV
1701	1711	1721	1731	1741	1751	1761	1771	1781	1791
DLPPGADPDK	EAAMMPAGVE	EGSSGDQPPY	LDAKPPTPGA	SFSQAESNVD	PEPDSTQPLS	KPAQKSEEAN	EPKAEKPDAT	ADAEPDANQK	AEAAPESQPP
1801	1811	1821	1831	1841	1851	1861	1871	1881	1891
ASEDLEVDPP	VAAKDKKPNK	SKRSKTPVQA	AAVSIVEKPV	TRKSERIDRE	KLKRSNSPRG	EAQKLLELKM	EAEKITRTAS	KNSAADLEHP	EPSLPLSRTR
1901	1911	1921	1931	1941	1951	1961	1971	1981	1991
RRNVRSVYAT	MGDHENRSPV	KEPVEQPRVT	RKRLERELQE	AAAVPTTPRR	GRPPKTRRRA	DEEEENEAKE	PAETLKPPEG	WRSPRSQKTA	AGGGPQGKKG
2001	2011	2021	2031	2041	2051	2061	2071	2081	2091
KNEPKVDATR	PEATTEVGPQ	IGVKESSMEP	KAAEEEAGSE	QKRDRKDAGT	DKNPPETAPV	EVVEKKPAPE	KNSKSKRGRS	RNSRLAVDKS	ASLKNVDAAV
2101	2111	2121	2131	2141	2151	2161	2171	2181	2191
SPRGAAAQAG	ERESGVVAVS	PEKSESPQKE	DGLSSQLKSD	PVDPDKEPEK	EDVSASGPSP	EATQLAKQME	LEQAVEHIAK	LAEASASAAY	KADAPEGLAP
2201	2211	2221	2231	2241	2251	2261	2271	2281	2291
EDRDKPAHQA	SETELAAAIG	SIINDISGEP	ENFPAPPPYP	GESQTDLQPP	AGAQALQPSE	EGMETDEAVS	GILETEAATE	SSRPPVNAPD	PSAGPTDTKE
2301	2311	2321	2331	2341	2351	2361	2371	2381	2391
ARGNSSETSH	SVPEAKGSKE	VEVTLVRKDK	GRQKTTRSRR	KRNTNKKVVA	PVESHVPESN	QAQGESPAAN	EGTTVQHPEA	PQEEKQSEKP	HSTPPQSCTS
2401	2411	2421	2431	2441	2451	2461	2471	2481	2491
DLSKIPSTEN	SSQEISVEER	TPTKASVPPD	LPPPPQPAPV	DEEPQARFRV	HSIIESDPVT	PPSDPSIPIP	TLPSVTAAKL	SPPVASGGIP	HQSPPTKVTE
2501	2511	2521	2531	2541	2551	2561	2571	2581	2591
WITRQEEPRA	QSTPSPALPP	DTKASDVDTS	SSTLRKILMD	PKYVSATSVT	STSVTTAIAE	PVSAAPCLHE	APPPPVDSKK	PLEEKTAPPV	TNNSEIQASE
2601	2611	2621	2631	2641	2651	2661	2671	2681	2691
VLVAADKEKV	APVIAPKITS	VISRMPVSID	LENSQKITLA	KPAPQTLTGL	VSALTGLVNV	SLVPVNALKG	PVKGSVTTLK	SLVSTPAGPV	NVLKGPVNVL
2701	2711	2721	2731	2741	2751	2761	2771	2781	2791
TGPVNVLTTP	VNATVGTVNA	APGTVNAAAS	AVNATASAVT	VTAGAVTAAS	GGVTATTGTV	TMAGAVIAPS	TKCKQRASAN	ENSRFHPGSM	PVIDDRPADA
2801	2811	2821	2831	2841	2851	2861	2871	2881	2891
GSGAGLRVNT	SEGVVLLSYS	GQKTEGPQRI	SAKISQIPPA	SAMDIEFQQS	VSKSQVKPDS	VTASQPPSKG	PQAPAGYANV	ATHSTLVLTA	QTYNASPVIS
2901	2911	2921	2931	2941	2951	2961	2971	2981	2991
SVKADRPSLE	KPEPIHLSVS	TPVTQGGTVK	VLTQGINTPP	VLVHNQLVLT	PSIVTTNKKL	ADPVTLKIET	KVLQPANLGS	TLTPHHPPAL	PSKLPTEVNH
3001	3011	3021	3031	3041	3051	3061	3071	3081	3091
VPSGPSIPAD	RTVSHLAAAK	LDAHSPRPSG	PGPSSFPRAS	HPSSTASTAL	STNATVMLAA	GIPVPQFISS	IHPEQSVIMP	PHSITQTVSL	SHLSQGEVRM
3101	3111	3121	3131	3141	3151	3161	3171	3181	3191
NTPTLPSITY	SIRPEALHSP	RAPLQPQQIE	VRAPQRASTP	QPAPAGVPAL	ASQHPPEEEV	HYHLPVARAT	APVQSEVLVM	QSEYRLHPYT	VPRDVRIMVH
3201	3211	3221	3231	3241	3251	3261	3271	3281	3291
PHVTAVSEQP	RAADGVVKVP	PASKAPQQPG	KEAAKTPDAK	AAPTPTPAPV	PVPVPLPAPA	PAPHGEARIL	TVTPSNQLQG	LPLTPPVVVT	HGVQIVHSSG
3301	3311	3321	3331	3341	3351	3361	3371	3381	3391
ELFQEYRYGD	IRTYHPPAQL	THTQFPAASS	VGLPSRTKTA	AQGPPPEGEP	LQPPQPVQST	QPAQPAPPCP	PSQLGQPGQP	PSSKMPQVSQ	EAKGTQTGVE
3401	3411	3421	3431	3441	3451	3461	3471	3481	3491
QPRLPAGPAN	RPPEPHTQVQ	RAQAETGPTS	FPSPVSVSMK	PDLPVSLPTQ	TAPKQPLFVP	TTSGPSTPPG	LVLPHTEFQP	APKQDSSPHL	TSQRPVDMVQ
3501	3511	3521	3531	3541	3551	3561	3571	3581	3591
LLKKYPIVWQ	GLLALKNDTA	AVQLHFVSGN	NVLAHRSLPL	SEGGPPLRIA	QRMRLEATQL	EGVARRMTVE	TDYCLLLALP	CGRDQEDVVS	QTESLKAAFI
3601	3611	3621	3631	3641	3651	3661
TYLQAKQAAG	IINVPNPGSN	QPAYVLQIFP	PCEFSESHLS	RLAPDLLASI	SNISPHLMIV	IASV

UniProtKB-Q96T58

Glycosites

Sequence

Reference

ID	Publication
1	Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
10	Tan ZJ, Yin HD, Nie S, Lin ZX, Zhu JH, Ruffin MT, Anderson MA, Simone DM, Lubman DM: Large-Scale Identification of Core-Fucosylated Glycopeptide Sites in Pancreatic Cancer Serum Using Mass Spectrometry. Journal of Proteome Research 2015, 14:1968-1978.
33	Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.