Contains      

UniProtKB-Q9HAT2

Protein Sialate O-acetylesterase
Gene SIAE
Status Reviewed
Source ARO Cell Line (Thyroid Cance); Bladder tumor; Breast Cancer cell lines; Breast cancer xenografts; Breast cell lines; Cerebrospinal fluid; Colorectal cancer cell line, HCT-116 (colorectal cancer); Colorectal tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); HCC cell lines (Liver); HCCLM3 cell line (Liver); IGROV-1/CP cell line (Ovarian cancer)IGROV-1/CP; Jurkat T cell line; LNCap/PC3 cell lines (Prostate cancer); Liver; Liver tumor (HCC); OVCAR-3 cell line (Ovarian cancer); Ovarian tumor; Pancreatic islets; Platelet; Prostate; Prostate tumor; Serum; TPC-1 Cell Line (Thyroid Cance); Urine; XTC-1 Cell Line (Thyroid Cance)
Years 2007-2015

Glycosites

Site Identified Peptides Year(Publication ID)
107 InFTLR 2007(22); 2014(33); 2014(2); 2015(unpublished);2015(unpublished);2015;2015;2007;2007;
107 LnFTIR 2007(unpublished);
107 LnFTLR 2015(1); 2015(unpublished);
107 TLEKInF 2014(33);
107 AQQTLEKInF 2014(33);
107 EVMAQQTLEKInF 2014(33);
107 QTIEKInFTIRVH 2013(37);
138 QIFnATR 2014(33);
138 IFnATREL 2014(33);
138 LQIFnATR 2007(unpublished);2007(unpublished);2007(unpublished);
138 QIFnATREL 2014(33);
138 VLQIFnATR 2014(14); 2015(unpublished);2015(unpublished);
138 TVLQIFnATR 2007(unpublished);2014(14); 2015(unpublished);
138 nATRELSNTAAY 2014(33);
138 QMTVLQIFnATR 2014(33);
138 MQMTVLQIFnATR 2014(14); 2014(33); 2015(unpublished);2015(unpublished);
138 QIFnATRELSNTAAY 2014(33);
138 CSGQSNMQMTVLQIFnATR 2014(33);
138 LCSGQSNMQMTVLQIFnATR 2014(33);
138 VHDVLFGDVWLCSGQSNMQMTVLQIFnATR 2012(52); 2015(unpublished);2015(2);
267 IHPLCnM 2014(33);
267 IHPLCnMTLK 2014(33); 2015(unpublished);
267 NAMIHPLCnM 2014(33);
267 AMIHPLCnMTLK 2012(48); 2015(unpublished);
267 NAMIHPLCnMTL 2014(33);
267 MIHPICnMTIKGV 2013(37);
267 NAMIHPLCnMTLK 2014(33);
267 HSVLWNAMIHPLCnM 2014(33);
267 NAMIHPLCnMTLKGVVW 2014(33);
267 HSVIWNAMIHPICnMTIK 2014(16);
267 HSVLWNAMIHPLCnMTLK 2007(58); 2011(48); 2012(45); 2013(33); 2014(2); 2015(unpublished);2015(unpublished);2007;2007;
290 nCTFPALIEDWR 2014(33);
290 NTDLYnCTFPAL 2014(33);
290 nCTFPALIEDWRE 2014(33);
290 nCTFPALIEDWRETF 2014(33);
290 NTDLYnCTFPALIEDWR 2014(33);
290 NTDLYnCTFPALIEDWRE 2014(33);
290 NYNTDLYnCTFPALIEDWR 2007(unpublished);2007 (Unpublished );
290 SNINYNTDLYnCTFPALIE 2014(33);
290 NTDLYnCTFPALIEDWRETF 2014(33);
290 SNINYNTDLYnCTFPALIEDWRE 2014(33);
290 GVVWYQGESNINYNTDLYnCTFPALIEDWR 2015(2);
401 AYGEKnLTF 2014(33);
401 nLTFEGPLPE 2014(33);
401 KnLTFEGPLPE 2014(33);
401 nLTFEGPLPEK 2007(63); 2009(64); 2009(52); 2012(40); 2013(45); 2013(14); 2014(22); 2014(33); 2014(1); 2015(2); 2015(unpublished);2015(unpublished);2015;2015;2015;2015;2007;2007;
401 GEKnLTFEGPLPE 2014(33);
401 IAYGEKnITFEGP 2013(37);
401 ALAYGEKnLTFEGPLPE 2014(33);
401 AIAYGEKnITFEGPIPEK 2014(32); 2014(32);
401 ALAYGEKnLTFEGPLPEK 2007(75); 2009(62); 2009(62); 2009(62); 2009(64); 2012(48); 2012(52); 2013(34); 2014(13); 2014(14); 2014(33); 2015(unpublished);2015(1); 2015(unpublished);2015(2); 2015(unpublished);2015(unpublished);2015(unpublished);
401 GEKnLTFEGPLPEKIELL 2014(33);
401 nLTFEGPLPEKIELLAHK 2009(64); 2013(45); 2014(33); 2015(2);
401 ALAYGEKnLTFEGPLPEKI 2014(14); 2015(unpublished);
401 AYGEKnLTFEGPLPEKIEL 2014(33);
401 AYGEKnLTFEGPLPEKIELL 2014(33);
401 AIAYGEKnITFEGPIPEKIEIIAHK 2014(16);
401 ALAYGEKnLTFEGPLPEKIELLAHK 2009(64); 2011(58); 2014(33); 2015(1); 2015(unpublished);2015(2);
422 AHKGLLnL 2014(33);
422 GLLnLTYY 2014(33);
422 AHKGLLnLTYY 2014(33);
422 nLTYYQQIQVQK 2015(1);
422 AHKGIInITYYQQ 2013(37);
422 GIInITYYQQIQVQK 2014(16);
422 GLLnLTYYQQIQVQK 2007(64); 2009(52); 2012(33); 2014(1); 2015(2); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015;2015;2007;2007;2007;2007;
422 GLLnLTYYQQIQVQKK 2014(33); 2015(unpublished);

Sequence

1112131415161718191
MVAPGLVLGLVLPLILWADRSAGIGFRFASYINNDMVLQKEPAGAVIWGFGTPGATVTVTLRQGQETIMKKVTSVKAHSDTWMVVLDPMKPGGPFEVMAQ
101111121131141151161171181191
QTLEKINFTLRVHDVLFGDVWLCSGQSNMQMTVLQIFNATRELSNTAAYQSVRILSVSPIQAEQELEDLVAVDLQWSKPTSENLGHGYFKYMSAVCWLFG
201211221231241251261271281291
RHLYDTLQYPIGLIASSWGGTPIEAWSSGRSLKACGVPKQGSIPYDSVTGPSKHSVLWNAMIHPLCNMTLKGVVWYQGESNINYNTDLYNCTFPALIEDW
301311321331341351361371381391
RETFHRGSQGQTERFFPFGLVQLSSDLSKKSSDDGFPQIRWHQTADFGYVPNPKMPNTFMAVAMDLCDRDSPFGSIHPRDKQTVAYRLHLGARALAYGEK
401411421431441451461471481491
NLTFEGPLPEKIELLAHKGLLNLTYYQQIQVQKKDNKIFEISCCSDHRCKWLPASMNTVSTQSLTLAIDSCHGTVVALRYAWTTWPCEYKQCPLYHPSSA
501511521
LPAPPFIAFITDQGPGHQSNVAK

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
45Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
52Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667.
58Nagano K, Shinkawa T, Kato K, Inomata N, Yabuki N, Haramura M: Distinct cell surface proteome profiling by biotin labeling and glycoprotein capturing. Journal of Proteomics 2011, 74:1985-1993.
62Arcinas A, Yen T-Y, Kebebew E, Macher BA: Cell Surface and Secreted Protein Profiles of Human Thyroid Cancer Cell Lines Reveal Distinct Glycoprotein Patterns. Journal of Proteome Research 2009, 8:3958-3968.
63Cao J, Shen C, Wang H, Shen H, Chen Y, Nie A, Yan G, Lu H, Liu Y, Yang P: Identification of N-Glycosylation Sites on Secreted Proteins of Human Hepatocellular Carcinoma Cells with a Complementary Proteomics Approach. Journal of Proteome Research 2009, 8:662-672.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
75Sun B, Ranish JA, Utleg AG, White JT, Yan X, Lin B, Hood L: Shotgun glycopeptide capture approach coupled with mass Spectrometry for comprehensive glycoproteomics. Molecular & Cellular Proteomics 2007, 6:141-149.