UniProtKB-Q9NTN9

Protein Semaphorin-4G
Gene SEMA4G
Status Reviewed
Source 22Rv1 cell xenograft (prostate cancer); Breast cancer xenografts; DLBCL cell lines (Diffuse large B-cell lymphoma); HCCLM3 cell line (Liver); HEK293 cell line (embryonic kidney); HuH-7 cell line (Liver); Liver; Liver tumor (HCC); OVCAR-3 cell line (Ovarian cancer)
Years 2009-2015

Glycosites

Site Identified Peptides Year(Publication ID)
55 GQAQnYSTLLLEE 2014(33);
55 nYSTLLLEEASAR 2015(1);
55 GQAQnYSTIIIEEASAR 2014(16); 2014(32); 2014(32);
55 GQAQnYSTLLLEEASAR 2013(34); 2014(33); 2015(1);
111 nQTECFNHVR 2015(1);
111 NnQTECFNHVR 2013(38); 2014(33);
111 GKNnQTECFNHVR 2014(33);
126 LnSTHLYACGTHAFQPLCAAIDAE 2014(33);
388 SQGYnSSQDIPSIVIDFVK 2014(32);
388 SQGYnSSQDLPSLVLDFVK 2009(63); 2012(50); 2013(34); 2014(15); 2014(33); 2015(1); 2015(unpublished);
542 DPYCGWDPGTHACAAATTIAnR 2014(33);
542 SCYDCILARDPYCGWDPGTHACAAATTIAnR 2013(38);
598 nGSMGLSDGQGGYR 2015(1);
598 LLnGSMGLSDGQGGYR 2014(33);
598 AIWIInGSMGISDGQGGYR 2014(32);
598 ALWLLnGSMGLSDGQGGYR 2014(33); 2015(1); 2015(unpublished);

Sequence

1112131415161718191
MWGRLWPLLLSILTATAVPGPSLRRPSRELDATPRMTIPYEELSGTRHFKGQAQNYSTLLLEEASARLLVGARGALFSLSANDIGDGAHKEIHWEASPEM
101111121131141151161171181191
QSKCHQKGKNNQTECFNHVRFLQRLNSTHLYACGTHAFQPLCAAIDAEAFTLPTSFEEGKEKCPYDPARGFTGLIIDGGLYTATRYEFRSIPDIRRSRHP
201211221231241251261271281291
HSLRTEETPMHWLNDAEFVFSVLVRESKASAVGDDDKVYYFFTERATEEGSGSFTQSRSSHRVARVARVCKGDLGGKKILQKKWTSFLKARLICHIPLYE
301311321331341351361371381391
TLRGVCSLDAETSSRTHFYAAFTLSTQWKTLEASAICRYDLAEIQAVFAGPYMEYQDGSRRWGRYEGGVPEPRPGSCITDSLRSQGYNSSQDLPSLVLDF
401411421431441451461471481491
VKLHPLMARPVVPTRGRPLLLKRNIRYTHLTGTPVTTPAGPTYDLLFLGTADGWIHKAVVLGSGMHIIEETQVFRESQSVENLVISLLQHSLYVGAPSGV
501511521531541551561571581591
IQLPLSSCSRYRSCYDCILARDPYCGWDPGTHACAAATTIANRTALIQDIERGNRGCESSRDTGPPPPLKTRSVLRGDDVLLPCDQPSNLARALWLLNGS
601611621631641651661671681691
MGLSDGQGGYRVGVDGLLVTDAQPEHSGNYGCYAEENGLRTLLASYSLTVRPATPAPAPKAPATPGAQLAPDVRLLYVLAIAALGGLCLILASSLLYVAC
701711721731741751761771781791
LREGRRGRRRKYSLGRASRAGGSAVQLQTVSGQCPGEEDEGDDEGAGGLEGSCLQIIPGEGAPAPPPPPPPPPPAELTNGLVALPSRLRRMNGNSYVLLR
801811821831
QSNNGVPAGPCSFAEELSRILEKRKHTQLVEQLDESSV

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
15Chen R, Seebun D, Ye M, Zou H, Figeys D: Site-specific characterization of cell membrane N-glycosylation with integrated hydrophilic interaction chromatography solid phase extraction and LC-MS/MS. Journal of Proteomics 2014, 103:194-203.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
38Kaji H, Ocho M, Togayachi A, Kuno A, Sogabe M, Ohkura T, Nozaki H, Angata T, Chiba Y, Ozaki H, et al: Glycoproteomic Discovery of Serological Biomarker Candidates for HCV/HBV Infection-Associated Liver Fibrosis and Hepatocellular Carcinoma. Journal of Proteome Research 2013, 12:2630-2640.
50Whitmore TE, Peterson A, Holzman T, Eastham A, Amon L, McIntosh M, Ozinsky A, Nelson PS, Martin DB: Integrative Analysis of N-Linked Human Glycoproteomic Data Sets Reveals PTPRF Ectodomain as a Novel Plasma Biomarker Candidate for Prostate Cancer. Journal of Proteome Research 2012, 11:2653-2665.
63Cao J, Shen C, Wang H, Shen H, Chen Y, Nie A, Yan G, Lu H, Liu Y, Yang P: Identification of N-Glycosylation Sites on Secreted Proteins of Human Hepatocellular Carcinoma Cells with a Complementary Proteomics Approach. Journal of Proteome Research 2009, 8:662-672.