Contains      

UniProtKB-Q9UHL4

Protein Dipeptidyl peptidase 2
Gene DPP7
Status Reviewed
Source ARO Cell Line (Thyroid Cance); Breast Cancer cell lines; Breast cancer cell lines; Breast cancer xenografts; Breast cell lines; Breast tumor; Cerebrospinal fluid; Colorectal cancer cell line, HCT-116 (colorectal cancer); DLBCL cell lines (Diffuse large B-cell lymphoma); DRO-1 Cell Line (Thyroid Cance); FTC-133 Cell Line (Thyroid Cance); HeLa cell line (Cervical cancer); Hela cell line (cervical cancer); HuH-7 cell line (Liver); IGROV-1/CP cell line (Ovarian cancer)IGROV-1/CP; LNCap/PC3 cell lines (Prostate cancer); Liver; Liver tumor (HCC); Non-small Cell Lung Carcinoma; OVCAR-3 cell line (Ovarian cancer); Ovarian cancer cell lines; Ovarian tumor; Pancreatic islets; Platelet; Prostate; Prostate cancer metastasis to liver; Prostate tumor; Spermatozoa; TPC-1 Cell Line (Thyroid Cance); Urine; UrineJurkat T cell line; XTC-1 Cell Line (Thyroid Cance)
Years 2007-2015

Glycosites

Site Identified Peptides Year(Publication ID)
50 FGnKTFPQR 2009(62); 2009(62); 2012(52); 2014(14); 2014(16); 2014(33); 2015(2); 2015(12); 2015(unpublished);2007(unpublished);2007;
50 GnKTFPQRF 2014(33);
50 FGnKTFPQRF 2009(64); 2014(33);
50 ERFGnKTFPQRF 2009(64);
50 LDHFNFERFGnK 2013(38);
50 NFERFGnKTFPQ 2014(33);
50 NFERFGnKTFPQRF 2014(33);
86 AnNSAFVAELAAERGALL 2014(33);
86 GEGPIFFYTGNEGDVWAFAnNSAFVAEIAAER 2013(43);
86 GEGPIFFYTGNEGDVWAFAnNSAFVAELAAER 2015(2);
315 nASGSEHCY 2014(33);
315 VYnASGSEHCY 2014(33);
315 nASGSEHCYDIY 2014(33);
315 ALAGLVYnASGSE 2014(33);
315 IAGIVYnASGSEH 2013(37);
315 nASGSEHCYDIYR 2012(48); 2014(33); 2015(1);
315 ALAGLVYnASGSEH 2014(33);
315 VYnASGSEHCYDIY 2014(33);
315 YnASGSEHCYDIYR 2012(48);
315 VYnASGSEHCYDIYR 2012(3); 2012(48); 2014(33);
315 ALAGLVYnASGSEHCY 2012(48); 2014(33);
315 AGLVYnASGSEHCYDIY 2014(33);
315 ALAGLVYnASGSEHCYD 2014(33);
315 GLVYnASGSEHCYDIYR 2012(48);
315 AGLVYnASGSEHCYDIYR 2012(48); 2014(14);
315 ALAGLVYnASGSEHCYDIY 2012(48); 2014(33);
315 AIAGIVYnASGSEHCYDIYR 2013(43); 2014(16); 2014(32);
315 ALAGLVYnASGSEHCYDIYR 2007(75); 2007(62); 2009(62); 2009(62); 2009(62); 2009(62); 2009(64); 2009(67); 2009(58); 2011(48); 2012(52); 2012(34); 2013(34); 2013(45); 2013(13); 2014(14); 2014(18); 2014(33); 2014(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2015(unpublished);2007(Unpublished ); 2007;2007;2007;2007;2007;2007;2007 ;
356 InLTFASNNVTD 2014(33);
356 InLTFASNNVTDMFPDLPFTDE 2014(33);
356 nLTFASNNVTDMFPDLPFTDELR 2007(unpublished);2007(unpublished);2007(unpublished);
356 InLTFASNNVTDMFPDLPFTDELR 2014(33);
363 INLTFASNnVTD 2014(33);
363 ASNnVTDMFPDLPF 2014(33);
363 nVTDMFPDLPFTDELR 2015(unpublished);
363 ASNnVTDMFPDLPFTDE 2014(33);
363 SNnVTDMFPDLPFTDELR 2007(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);
363 ASNnVTDMFPDLPFTDELR 2014(33); 2015(unpublished);
363 ASNnVTDMFPDLPFTDELRQRY 2014(33);
363 INLTFASNnVTDMFPDLPFTDE 2014(33);
363 NLTFASNnVTDMFPDLPFTDELR 2007(unpublished);2007(unpublished);2007(unpublished);
363 INLTFASNnVTDMFPDLPFTDELR 2014(33);
428 GGGIRRnISASVI 2013(37);
428 nLSASVIAVTIQGGAHHL 2014(33);
428 RnLSASVIAVTIQGGAHHL 2014(33);
428 nLSASVIAVTIQGGAHHLDLR 2012(52); 2014(33); 2015(unpublished);2015(unpublished);2015(2); 2015(unpublished);2015(12);
428 RnISASVIAVTIQGGAHHIDIR 2013(43); 2014(16);
428 RnLSASVIAVTIQGGAHHLDLR 2009(62); 2012(52); 2014(33); 2015(unpublished);2015(1); 2015(unpublished);2015(2); 2015(unpublished);

Sequence

1112131415161718191
MGSAPWAPVLLLALGLRGLQAGARRAPDPGFQERFFQQRLDHFNFERFGNKTFPQRFLVSDRFWVRGEGPIFFYTGNEGDVWAFANNSAFVAELAAERGA
101111121131141151161171181191
LLVFAEHRYYGKSLPFGAQSTQRGHTELLTVEQALADFAELLRALRRDLGAQDAPAIAFGGSYGGMLSAYLRMKYPHLVAGALAASAPVLAVAGLGDSNQ
201211221231241251261271281291
FFRDVTADFEGQSPKCTQGVREAFRQIKDLFLQGAYDTVRWEFGTCQPLSDEKDLTQLFMFARNAFTVLAMMDYPYPTDFLGPLPANPVKVGCDRLLSEA
301311321331341351361371381391
QRITGLRALAGLVYNASGSEHCYDIYRLYHSCADPTGCGTGPDARAWDYQACTEINLTFASNNVTDMFPDLPFTDELRQRYCLDTWGVWPRPDWLLTSFW
401411421431441451461471481491
GGDLRAASNIIFSNGNLDPWAGGGIRRNLSASVIAVTIQGGAHHLDLRASHPEDPASVVEARKLEATIIGEWVKAARREQQPALRGGPRLSL

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
3Tian Y, Almaraz RT, Choi CH, Li QK, Saeui C, Li D, Shah P, Bhattacharya R, Yarema KJ, Zhang H: Identification of sialylated glycoproteins from metabolically oligosaccharide engineered pancreatic cells. Clinical proteomics 2015, 12:11.
12Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
18Hirao Y, Matsuzaki H, Iwaki J, Kuno A, Kaji H, Ohkura T, Togayachi A, Abe M, Nomura M, Noguchi M, et al: Glycoproteomics Approach for Identifying Glycobiomarker Candidate Molecules for Tissue Type Classification of Non-small Cell Lung Carcinoma. Journal of Proteome Research 2014, 13:4705-4716.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
38Kaji H, Ocho M, Togayachi A, Kuno A, Sogabe M, Ohkura T, Nozaki H, Angata T, Chiba Y, Ozaki H, et al: Glycoproteomic Discovery of Serological Biomarker Candidates for HCV/HBV Infection-Associated Liver Fibrosis and Hepatocellular Carcinoma. Journal of Proteome Research 2013, 12:2630-2640.
43Wang GG, Wu YB, Zhou T, Guo YS, Zheng B, Wang J, Bi Y, Liu FJ, Zhou ZM, Guo XJ, Sha JH: Mapping of the N-Linked Glycoproteome of Human Spermatozoa. Journal of Proteome Research 2013, 12:5750-5759.
45Zhou H, Froehlich JW, Briscoe AC, Lee RS: The GlycoFilter: A Simple and Comprehensive Sample Preparation Platform for Proteomics, N-Glycomics and Glycosylation Site Assignment. Molecular & Cellular Proteomics 2013, 12:2981-2991.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
52Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667.
58Nagano K, Shinkawa T, Kato K, Inomata N, Yabuki N, Haramura M: Distinct cell surface proteome profiling by biotin labeling and glycoprotein capturing. Journal of Proteomics 2011, 74:1985-1993.
62Arcinas A, Yen T-Y, Kebebew E, Macher BA: Cell Surface and Secreted Protein Profiles of Human Thyroid Cancer Cell Lines Reveal Distinct Glycoprotein Patterns. Journal of Proteome Research 2009, 8:3958-3968.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
67McDonald CA, Yang JY, Marathe V, Yen T-Y, Macher BA: Combining Results from Lectin Affinity Chromatography and Glycocapture Approaches Substantially Improves the Coverage of the Glycoproteome. Molecular & Cellular Proteomics 2009, 8:287-301.
75Sun B, Ranish JA, Utleg AG, White JT, Yan X, Lin B, Hood L: Shotgun glycopeptide capture approach coupled with mass Spectrometry for comprehensive glycoproteomics. Molecular & Cellular Proteomics 2007, 6:141-149.