Contains      

UniProtKB-Q9UIQ6

Protein Leucyl-cystinyl aminopeptidase
Gene LNPEP
Status Reviewed
Source Breast Cancer cell lines; Breast cancer cell lines; Breast cancer xenografts; Breast cell lines; Breast tumor; Colorectal cancer cell line, HCT-116 (colorectal cancer); Colorectal tumor; DLBCL cell lines (Diffuse large B-cell lymphoma); HCC cell lines (Liver); HCC cell lines (Liver)Jurkat T cell line; HEK293T cell line (embryonic kidney); HEK293T cell line (embryonic kidney), HEK293T cell line (embryonic kidney); Hela cell line (cervical cancer); IGROV-1/CP cell line (Ovarian cancer)IGROV-1/CP; Jurkat T cell line; K562A cell line (Leukemia); K562S cell line (Leukemia); LNCap/PC3 cell lines (Prostate cancer); Liver; Liver tumor (HCC); Lymphocytes; OVCAR-3 cell line (Ovarian cancer); OVCAR-3 cell line (Ovarian cancer)Jurkat T cell line; Ovarian tumor; Pancreatic islets; Platelet; Platelet Plasma membranes; Prostate; Prostate tumor; SW1990 cell line (Pancreatic cancer); Serum; Urine; ovarian tumor; prostate cancer cell lines
Years 2007-2015

Glycosites

Site Identified Peptides Year(Publication ID)
145 KnQSIGLIQPF 2014(33);
145 nQSIGLIQPFATNGK 2007(72); 2011(57); 2014(13); 2015(unpublished);2015(1); 2015(2); 2015(unpublished);2015(12);
145 KnQSIGIIQPFATNGK 2014(16);
145 KnQSIGLIQPFATNGK 2014(13); 2014(33); 2015(unpublished);2015(1); 2015(2); 2015(12);
184 nLTSMTFR 2015(1);
184 ELSLHPnLTSM 2014(33);
184 LSLHPnLTSMTF 2014(33);
184 EISIHPnITSMTF 2013(37);
184 ELSLHPnLTSMTF 2014(33);
184 YEISIHPnITSMTFR 2014(16);
184 YELSLHPnLTSMTFR 2012(48); 2013(40); 2014(14); 2015(unpublished);2015(1); 2015(2); 2015(unpublished);2015(unpublished);
215 HSTGHnISR 2012(48);
215 NIILHSTGHnISRVTF 2014(33);
256 ALLAGHnY 2014(33);
256 LAGHnYTLK 2015(unpublished);
256 ALLAGHnYTLK 2014(33);
256 ALLAGHnYTLKIE 2014(33);
256 IAIVAPEALLAGHnYTLK 2015(unpublished);
256 AYHGQIAIVAPEALLAGHnY 2014(33);
256 QAEIIEYAYHGQIAIVAPEAIIAGHnYTIK 2014(16);
256 QAEILEYAYHGQIAIVAPEALLAGHnYTLK 2012(52); 2015(unpublished);2015(1); 2015(2);
266 SAnISSSYY 2014(33);
368 nISQDVNGTIVSIYAVPEK 2014(16);
368 nLSQDVNGTLVSIYAVPEK 2012(52); 2013(40); 2015(unpublished);2015(2); 2015(unpublished);2015(12);
374 nGTLVSIYAVPEK 2015(1);
374 NISQDVnGTIVSIYAVPEK 2014(16);
374 NLSQDVnGTLVSIYAVPEK 2012(52); 2013(40); 2015(unpublished);2015(2); 2015(unpublished);2015(12);
448 nTSSMADR 2015(1);
448 nTSSMADRK 2015(1);
448 TLLYDSnTSSMADR 2014(33);
448 EETIIYDSnTSSMADR 2014(32); 2014(32);
448 EETLLYDSnTSSMADR 2007(72); 2007(75); 2011(54); 2012(47); 2012(48); 2012(52); 2013(34); 2013(40); 2014(13); 2014(14); 2014(22); 2014(25); 2014(33); 2015(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2015;2015;2007;2007;2007;
448 EETIIYDSnTSSMADRK 2014(16);
448 EETLLYDSnTSSMADRK 2007(72); 2014(13); 2015(1); 2015(12);
525 DSLnSSHPI 2014(14); 2015(unpublished);
525 DSLnSSHPISS 2014(33);
525 DSLnSSHPISSS 2014(33);
525 DSLnSSHPISSSVQ 2014(14); 2015(unpublished);
525 DSLnSSHPISSSVQSSEQIEE 2014(33);
525 KDSLnSSHPISSSVQSSEQIEE 2014(33);
525 DSInSSHPISSSVQSSEQIEEMFDSISYFK 2014(32); 2014(32);
525 DSLnSSHPISSSVQSSEQIEEMFDSLSYFK 2011(57); 2012(52); 2013(34); 2014(25); 2014(25); 2014(33); 2015(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007;2007;
525 KDSInSSHPISSSVQSSEQIEEMFDSISYFK 2014(16);
525 KDSLnSSHPISSSVQSSEQIEEMFDSLSYFK 2015(unpublished);2015(1); 2015(2); 2015(12);
598 VTnQTLDVK 2014(33);
598 VTnQTLDVKR 2014(33);
598 NEVTnQTLDVK 2014(14); 2007(unpublished);2007(unpublished);
664 SYVTEGRnY 2014(33);
664 VTEGRnYSKY 2014(33);
664 SYVTEGRnYSKY 2014(33);
682 SGVInLTEE 2014(33);
682 SGVInITEEVIWVK 2014(16); 2014(32); 2014(32);
682 SGVInLTEEVLWVK 2007(72); 2007(74); 2011(57); 2012(52); 2014(25); 2014(25); 2014(33); 2015(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015;2007;2007;
682 DKKSGVInLTEEVLW 2014(33);
682 KSGVInLTEEVLWVK 2015(1);
760 AFDLINYLGNEnHTAPITEALFQTDLIYNLLEK 2011(58); 2015(2); 2015(9);
834 nCSTTAMK 2015(1);
834 SAIIEFACTHNIGnCSTTAMK 2014(16); 2014(32);
834 SALLEFACTHNLGnCSTTAMK 2011(57); 2011(58); 2012(52); 2013(34); 2014(13); 2014(25); 2014(25); 2014(33); 2015(1); 2015(12); 2015(unpublished);2015(unpublished);2007(unpublished);2007(unpublished);2007(unpublished);2007;2007;
850 nGTQSLPTDVMTTVFK 2015(1);
850 IFDDWMASnGTQSIPTDVMTTVFK 2014(16);
850 LFDDWMASnGTQSLPTDVMTTVFK 2011(57); 2012(48); 2012(52); 2014(25); 2015(1); 2015(2); 2015(12); 2015(unpublished);2015(unpublished);2015(unpublished);2007(unpublished);2007;2007;2007;
989 THLSEVQAFFEnQSEATFR 2012(52); 2015(unpublished);

Sequence

1112131415161718191
MEPFTNDRLQLPRNMIENSMFEEEPDVVDLAKEPCLHPLEPDEVEYEPRGSRLLVRGLGEHEMEEDEEDYESSAKLLGMSFMNRSSGLRNSATGYRQSPD
101111121131141151161171181191
GACSVPSARTMVVCAFVIVVAVSVIMVIYLLPRCTFTKEGCHKKNQSIGLIQPFATNGKLFPWAQIRLPTAVVPLRYELSLHPNLTSMTFRGSVTISVQA
201211221231241251261271281291
LQVTWNIILHSTGHNISRVTFMSAVSSQEKQAEILEYAYHGQIAIVAPEALLAGHNYTLKIEYSANISSSYYGFYGFSYTDESNEKKYFAATQFEPLAAR
301311321331341351361371381391
SAFPCFDEPAFKATFIIKIIRDEQYTALSNMPKKSSVVLDDGLVQDEFSESVKMSTYLVAFIVGEMKNLSQDVNGTLVSIYAVPEKIGQVHYALETTVKL
401411421431441451461471481491
LEFFQNYFEIQYPLKKLDLVAIPDFEAGAMENWGLLTFREETLLYDSNTSSMADRKLVTKIIAHELAHQWFGNLVTMKWWNDLWLNEGFATFMEYFSLEK
501511521531541551561571581591
IFKELSSYEDFLDARFKTMKKDSLNSSHPISSSVQSSEQIEEMFDSLSYFKGSSLLLMLKTYLSEDVFQHAVVLYLHNHSYASIQSDDLWDSFNEVTNQT
601611621631641651661671681691
LDVKRMMKTWTLQKGFPLVTVQKKGKELFIQQERFFLNMKPEIQPSDTSYLWHIPLSYVTEGRNYSKYQSVSLLDKKSGVINLTEEVLWVKVNINMNGYY
701711721731741751761771781791
IVHYADDDWEALIHQLKINPYVLSDKDRANLINNIFELAGLGKVPLKRAFDLINYLGNENHTAPITEALFQTDLIYNLLEKLGYMDLASRLVTRVFKLLQ
801811821831841851861871881891
NQIQQQTWTDEGTPSMRELRSALLEFACTHNLGNCSTTAMKLFDDWMASNGTQSLPTDVMTTVFKVGAKTDKGWSFLLGKYISIGSEAEKNKILEALASS
901911921931941951961971981991
EDVRKLYWLMKSSLNGDNFRTQKLSFIIRTVGRHFPGHLLAWDFVKENWNKLVQKFPLGSYTIQNIVAGSTYLFSTKTHLSEVQAFFENQSEATFRLRCV
100110111021
QEALEVIQLNIQWMEKNLKSLTWWL

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
2Shah P, Wang X, Yang W, Eshghi ST, Sun S, Hoti N, Chen L, Yang S, Pasay J, Rubin A: Integrated Proteomic and Glycoproteomic Analyses of Prostate Cancer Cells Reveal Glycoprotein Alteration in Protein Abundance and Glycosylation. Molecular & Cellular Proteomics 2015, 14:2753-2763.
9Smeekens JM, Chen WX, Wu RH: Mass Spectrometric Analysis of the Cell Surface N-Glycoproteome by Combining Metabolic Labeling and Click Chemistry. Journal of the American Society for Mass Spectrometry 2015, 26:604-614.
12Weng Y, Sui Z, Jiang H, Shan Y, Chen L, Zhang S, Zhang L, Zhang Y: Releasing N-glycan from Peptide N-terminus by N-terminal Succinylation Assisted Enzymatic Deglycosylation. Scientific Reports 2015, 5.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
16Deeb SJ, Cox J, Schmidt-Supprian M, Mann M: N-linked Glycosylation Enrichment for In-depth Cell Surface Proteomics of Diffuse Large B-cell Lymphoma Subtypes. Molecular & Cellular Proteomics 2014, 13:240-251.
22Nicastri A, Gaspari M, Sacco R, Elia L, Gabriele C, Romano R, Rizzuto A, Cuda G: N-Glycoprotein Analysis Discovers New Up-Regulated Glycoproteins in Colorectal Cancer Tissue. Journal of Proteome Research 2014, 13:4932-4941.
25Sun Z, Dong J, Zhang S, Hu Z, Cheng K, Li K, Xu B, Ye M, Nie Y, Fan D, Zou H: Identification of Chemoresistance-Related Cell-Surface Glycoproteins in Leukemia Cells and Functional Validation of Candidate Glycoproteins. Journal of Proteome Research 2014, 13:1593-1601.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
34Sun S, Zhang B, Aiyetan P, Zhou J-Y, Shah P, Yang W, Levine DA, Zhang Z, Chan DW, Zhang H: Analysis of N-glycoproteins using Genomic N-glycosite Prediction. Journal of Proteome Research 2013, 12:5609-5615.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
47Almaraz RT, Tian Y, Bhattarcharya R, Tan E, Chen S-H, Dallas MR, Chen L, Zhang Z, Zhang H, Konstantopoulos K: Metabolic flux increases glycoprotein sialylation: implications for cell adhesion and cancer metastasis. Molecular & Cellular Proteomics 2012, 11:M112. 017558.
48Danzer C, Eckhardt K, Schmidt A, Fankhauser N, Ribrioux S, Wollscheid B, Mueller L, Schiess R, Zuellig R, Lehmann R, et al: Comprehensive Description of the N-Glycoproteome of Mouse Pancreatic beta-Cells and Human Islets. Journal of Proteome Research 2012, 11:1598-1608.
52Yen T-Y, Macher BA, McDonald CA, Alleyne-Chin C, Timpe LC: Glycoprotein Profiles of Human Breast Cells Demonstrate a Clear Clustering of Normal/Benign versus Malignant Cell Lines and Basal versus Luminal Cell Lines. Journal of Proteome Research 2012, 11:656-667.
54Tian Y, Yao Z, Roden R, Zhang H: Identification of glycoproteins associated with different histological subtypes of ovarian tumors using quantitative glycoproteomics. Proteomics 2011, 11:4677-4687.
57Chen Y, Cao J, Yan G, Lu H, Yang P: Two-step protease digestion and glycopeptide capture approach for accurate glycosite identification and glycoprotein sequence coverage improvement. Talanta 2011, 85:70-75.
58Nagano K, Shinkawa T, Kato K, Inomata N, Yabuki N, Haramura M: Distinct cell surface proteome profiling by biotin labeling and glycoprotein capturing. Journal of Proteomics 2011, 74:1985-1993.
72Hanson SR, Hsu T-L, Weerapana E, Kishikawa K, Simon GM, Cravatt BF, Wong C-H: Tailored glycoproteomics and glycan site mapping using saccharide-selective bioorthogonal probes. Journal of the American Chemical Society 2007, 129:7266-+.
74Lewandrowski U, Zahedi RP, Moebius J, Walter U, Sickmann A: Enhanced N-glycosylation site analysis of Sialoglycopeptides by strong cation exchange prefractionation applied to platelet plasma membranes. Molecular & Cellular Proteomics 2007, 6:1933-1941.
75Sun B, Ranish JA, Utleg AG, White JT, Yan X, Lin B, Hood L: Shotgun glycopeptide capture approach coupled with mass Spectrometry for comprehensive glycoproteomics. Molecular & Cellular Proteomics 2007, 6:141-149.