Contains      

UniProtKB-Q9Y5C1

Protein Angiopoietin-related protein 3
Gene ANGPTL3
Status Reviewed
Source Bladder stromal cell line; Breast Cancer cell lines; Cerebrospinal fluid; HCC cell lines (Liver); HuH-7 cell line (Liver); Liver; Liver tumor (HCC); OVCAR-3 cell line (Ovarian cancer); Plasma; Prostate; Prostate stromal cell line; Prostate tumor; Serum; Urine
Years 2005-2015

Glycosites

Site Identified Peptides Year(Publication ID)
23 IDQDnSSFDSLSPEPK 2005( 81); 2013(40); 2014(33); 2015(unpublished);2015(unpublished);2015(unpublished);2007;2007;2007;
23 SRIDQDnSSFDSLSPEPK 2014(33);
23 SRIDQDnSSFDSLSPEPKSRF 2014(33);
115 VKnMSLE 2014(33);
115 nMSLELNSK 2013(38);
115 NEEVKnMSLE 2014(33);
115 NEEVKnMSLELNSK 2005( 81); 2007(13); 2014(14); 2014(33); 2014(unpublished);2015(unpublished);2015(unpublished);2015;2015;2015;2007;2007;2007;
115 NEEVKnMSLELNSKLESLLEEK 2013(38);
296 DGSQNFnETWENY 2013(37);
296 IDGSQNFnETWENYK 2005( 81); 2007(64); 2009(65); 2009(65); 2009(38); 2013(40); 2013(32); 2014(33); 2014(1); 2015(unpublished);2015(unpublished);2015(unpublished);2015;2007;2007;2007;
296 RIDGSQNFnETWENY 2014(33);
296 IDGSQNFnETWENYKYG 2009(64);
296 TLIQHRIDGSQNFnETWENY 2014(33);
357 LGNHETnY 2014(33);
357 LGNHETnYTLH 2014(33);
357 SFYLGNHETnY 2014(33);
357 LGNHETnYTLHLVAITGNVPNAIPENK 2014(33);

Sequence

1112131415161718191
MFTIKLLLFIVPLVISSRIDQDNSSFDSLSPEPKSRFAMLDDVKILANGLLQLGHGLKDFVHKTKGQINDIFQKLNIFDQSFYDLSLQTSEIKEEEKELR
101111121131141151161171181191
RTTYKLQVKNEEVKNMSLELNSKLESLLEEKILLQQKVKYLEEQLTNLIQNQPETPEHPEVTSLKTFVEKQDNSIKDLLQTVEDQYKQLNQQHSQIKEIE
201211221231241251261271281291
NQLRRTSIQEPTEISLSSKPRAPRTTPFLQLNEIRNVKHDGIPAECTTIYNRGEHTSGMYAIRPSNSQVFHVYCDVISGSPWTLIQHRIDGSQNFNETWE
301311321331341351361371381391
NYKYGFGRLDGEFWLGLEKIYSIVKQSNYVLRIELEDWKDNKHYIEYSFYLGNHETNYTLHLVAITGNVPNAIPENKDLVFSTWDHKAKGHFNCPEGYSG
401411421431441451
GWWWHDECGENNLNGKYNKPRAKSKPERRRGLSWKSQNGRLYSIKSTKMLIHPTDSESFE

Reference

ID Publication
1Sun S, Shah P, Eshghi ST, Yang W, Trikannad N, Yang S, Chen L, Aiyetan P, Hoti N, Zhang Z, et al: Comprehensive analysis of protein glycosylation by solid-phase extraction of N-linked glycans and glycosite-containing peptides. Nat Biotech 2015, advance online publication.
13Yang W, Laeyendecker O, Wendel SK, Zhang B, Sun S, Zhou J-Y, Ao M, Moore RD, Jackson JB, Zhang H: Glycoproteomic Study Reveals Altered Plasma Proteins Associated with HIV Elite Suppressors. Theranostics 2014, 4:1153.
14Liu Y, Chen J, Sethi A, Li QK, Chen L, Collins B, Gillet LC, Wollscheid B, Zhang H, Aebersold R: Glycoproteomic analysis of prostate cancer tissues by SWATH mass spectrometry discovers N-acylethanolamine acid amidase and protein tyrosine kinase 7 as signatures for tumor aggressiveness. Molecular & Cellular Proteomics 2014, 13:1753-1768.
32Zhang Z, Sun Z, Zhu J, Liu J, Huang G, Ye M, Zou H: High-Throughput Determination of the Site-Specific N-Sialoglycan Occupancy Rates by Differential Oxidation of Glycoproteins Followed with Quantitative Glycoproteomics Analysis. Analytical Chemistry 2014, 86:9830-9837.
33Zhu J, Sun Z, Cheng K, Chen R, Ye M, Xu B, Sun D, Wang L, Liu J, Wang F, Zou H: Comprehensive Mapping of Protein N-Glycosylation in Human Liver by Combining Hydrophilic Interaction Chromatography and Hydrazide Chemistry. Journal of Proteome Research 2014, 13:1713-1721.
37Boersema PJ, Geiger T, Wisniewski JR, Mann M: Quantification of the N-glycosylated Secretome by Super-SILAC During Breast Cancer Progression and in Human Blood Samples. Molecular & Cellular Proteomics 2013, 12:158-171.
38Kaji H, Ocho M, Togayachi A, Kuno A, Sogabe M, Ohkura T, Nozaki H, Angata T, Chiba Y, Ozaki H, et al: Glycoproteomic Discovery of Serological Biomarker Candidates for HCV/HBV Infection-Associated Liver Fibrosis and Hepatocellular Carcinoma. Journal of Proteome Research 2013, 12:2630-2640.
40Li X, Jiang J, Zhao X, Wang J, Han H, Zhao Y, Peng B, Zhong R, Ying W, Qian X: N-glycoproteome Analysis of the Secretome of Human Metastatic Hepatocellular Carcinoma Cell Lines Combining Hydrazide Chemistry, HILIC Enrichment and Mass Spectrometry. Plos One 2013, 8.
64Chen R, Jiang X, Sun D, Han G, Wang F, Ye M, Wang L, Zou H: Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry. J Proteome Res 2009, 8:651-661.
65Goo YA, Lilu AY, Ryu S, Shaffer SA, Malmstrom L, Page L, Nguyen LT, Doneanu CE, Goodlett DR: Identification of Secreted Glycoproteins of Human Prostate and Bladder Stromal Cells by Comparative Quantitative Proteomics. Prostate 2009, 69:49-61.